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ART do not increase the risk of Y-chromosome microdeletion in 19 candidate genes at AZF regions

ART do not increase the risk of Y-chromosome microdeletion in 19 candidate genes at AZF regions Y-chromosome microdeletions (YCMs) have been found at a much higher rate in infertile men than fertile controls. A specific deletion in the azoospermia factor locus (AZF) at Yq11 is significantly associated with male infertility. Whether assisted reproductive technology (ART) increases the risk of YCM in ART-derived offspring remains unclear. In this study the occurrence of YCM in 199 fathers and their 228 sons (Chinese, Han ethnicity), including 85 offspring conceived by IVF, 73 by intra-cytoplasmic sperm injection (ICSI) and 70 by natural conception, was investigated. Nineteen candidate genes related to YCM were analysed by multiplex ligation-dependent probe amplification. We identified one de novo YCM from 70 naturally-conceived offspring and none from 158 ART-conceived offspring and found no statistical significance between these two groups. There was no statistically-significant difference in the detection rate of the fathers Y-chromosome microdeletion group: IVF 10.7 (8/75), ICSI 3.2 (2/63), natural conception 8.2 (5/61). These results suggest that ART does not increase the risk of YCM in male offspring. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Reproduction Fertility and Development CSIRO Publishing

ART do not increase the risk of Y-chromosome microdeletion in 19 candidate genes at AZF regions

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Publisher
CSIRO Publishing
Copyright
Copyright © The Author(s). Published by CSIRO Publishing
ISSN
1031-3613
eISSN
1448-5990
DOI
10.1071/RD13092
pmid
23751883
Publisher site
See Article on Publisher Site

Abstract

Y-chromosome microdeletions (YCMs) have been found at a much higher rate in infertile men than fertile controls. A specific deletion in the azoospermia factor locus (AZF) at Yq11 is significantly associated with male infertility. Whether assisted reproductive technology (ART) increases the risk of YCM in ART-derived offspring remains unclear. In this study the occurrence of YCM in 199 fathers and their 228 sons (Chinese, Han ethnicity), including 85 offspring conceived by IVF, 73 by intra-cytoplasmic sperm injection (ICSI) and 70 by natural conception, was investigated. Nineteen candidate genes related to YCM were analysed by multiplex ligation-dependent probe amplification. We identified one de novo YCM from 70 naturally-conceived offspring and none from 158 ART-conceived offspring and found no statistical significance between these two groups. There was no statistically-significant difference in the detection rate of the fathers Y-chromosome microdeletion group: IVF 10.7 (8/75), ICSI 3.2 (2/63), natural conception 8.2 (5/61). These results suggest that ART does not increase the risk of YCM in male offspring.

Journal

Reproduction Fertility and Development – CSIRO Publishing

Published: Jun 11, 2013

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