Access the full text.
Sign up today, get DeepDyve free for 14 days.
R. Meier, Guanyang Zhang, F. Ali (2008)
The use of mean instead of smallest interspecific distances exaggerates the size of the "barcoding gap" and leads to misidentification.Systematic biology, 57 5
K. Meiklejohn, J. Wallman, M. Dowton (2009)
DNA-based identification of forensically important Australian Sarcophagidae (Diptera)International Journal of Legal Medicine, 125
T. Whitworth, R. Dawson, H. Magalon, E. Baudry (2007)
DNA barcoding cannot reliably identify species of the blowfly genus Protocalliphora (Diptera: Calliphoridae)Proceedings of the Royal Society B: Biological Sciences, 274
P. Hebert, E. Penton, J. Burns, D. Janzen, W. Hallwachs (2004)
Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator.Proceedings of the National Academy of Sciences of the United States of America, 101 41
(1954)
Contribution to the knowledge of the Australian sarcophagid flies belonging to the genus “Tricholioproctia” Baranov, 1938 (Diptera)
R. Meier, Kwong Shiyang, Gaurav Vaidya, P. Ng (2006)
DNA barcoding and taxonomy in Diptera: a tale of high intraspecific variability and low identification success.Systematic biology, 55 5
K. Tamura, J. Dudley, M. Nei, Sudhir Kumar (2007)
MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0.Molecular biology and evolution, 24 8
Meiklejohn K. A. Wallman J. F. Dowton M.
DNA-based identification of forensically important Australian
R. Zehner, J. Amendt, Svenja Schütt, Jan Sauer, R. Krettek, D. Povolný (2004)
Genetic identification of forensically important flesh flies (Diptera: Sarcophagidae)International Journal of Legal Medicine, 118
E. Catts, M. Goff (1992)
Forensic entomology in criminal investigations.Annual review of entomology, 37
M. Cummings (2004)
PAUP* [Phylogenetic Analysis Using Parsimony (and Other Methods)]Dictionary of Bioinformatics and Computational Biology
E. Catts (1992)
Problem in estimating the postmortem interval in death investigations
R. Nielsen, M. Matz (2006)
Statistical approaches for DNA barcoding.Systematic biology, 55 1
M. Moulton, Hojun Song, M. Whiting (2010)
Assessing the effects of primer specificity on eliminating numt coamplification in DNA barcoding: a case study from Orthoptera (Arthropoda: Insecta)Molecular Ecology Resources, 10
S. Aljanabi, Iciar Martinez (1997)
Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques.Nucleic acids research, 25 22
F. Patel (1990)
Forensic entomology.Forensic science international, 45 1-2
T. Pons, B. González, F. Ceciliani, A. Galizzi (2006)
FlgM anti-sigma factors: identification of novel members of the family, evolutionary analysis, homology modeling, and analysis of sequence-structure-function relationshipsJournal of Molecular Modeling, 12
A. Draber-Mońko, T. Malewski, J. Pomorski, M. Łoś, P. Ślipiński (2009)
On the Morphology and Mitochondrial DNA Barcoding of the Flesh Fly Sarcophaga (Liopygia) Argyrostoma (Robineau-Desvoidy, 1830) (Diptera: Sarcophagidae) — An Important Species in Forensic Entomology, 59
E. Tavares, A. Baker (2008)
Single mitochondrial gene barcodes reliably identify sister-species in diverse clades of birdsBMC Evolutionary Biology, 8
M. Matz, R. Nielsen (2005)
A likelihood ratio test for species membership based on DNA sequence dataPhilosophical Transactions of the Royal Society B: Biological Sciences, 360
R. Ward, T. Zemlak, B. Innes, P. Last, P. Hebert (2005)
DNA barcoding Australia's fish speciesPhilosophical Transactions of the Royal Society B: Biological Sciences, 360
J. Rivera, D. Currie (2009)
Identification of Nearctic black flies using DNA barcodes (Diptera: Simuliidae)Molecular Ecology Resources, 9
Ekrem T. Stur E. Hebert P. D. N.
Females do count: documenting
I. Hogg, P. Hebert (2004)
Biological identification of springtails (Hexapoda: Collembola) from the Canadian Arctic, using mitochondrial DNA barcodesCanadian Journal of Zoology, 82
R. Blackith, R. Blackith (1992)
Collecting sarcophagine flies on summits
Ball S. L. Hebert P. D. N. Burian S. K. Webb J. M.
Biological identifications of
D. Yeates, A. Seago, L. Nelson, S. Cameron, L. Joseph, J. Trueman (2011)
Integrative taxonomy, or iterative taxonomy?Systematic Entomology, 36
Hojun Song, J. Buhay, M. Whiting, K. Crandall (2008)
Many species in one: DNA barcoding overestimates the number of species when nuclear mitochondrial pseudogenes are coamplifiedProceedings of the National Academy of Sciences, 105
P. Hebert, A. Cywinska, S. Ball, Jeremy deWaard (2003)
Biological identifications through DNA barcodesProceedings of the Royal Society of London. Series B: Biological Sciences, 270
L. Nelson, J. Wallman, M. Dowton (2007)
Using COI barcodes to identify forensically and medically important blowfliesMedical and Veterinary Entomology, 21
S. Ball, P. Hebert, S. Burian, J. Webb (2005)
Biological identifications of mayflies (Ephemeroptera) using DNA barcodes, 24
T. Ekrem, E. Stúr, P. Hebert (2010)
Females do count: Documenting Chironomidae (Diptera) species diversity using DNA barcodingOrganisms Diversity & Evolution, 10
Lopes H. S.
A revision of Australian
J. Wells, T. Pape, F. Sperling (2001)
DNA-based identification and molecular systematics of forensically important Sarcophagidae (Diptera).Journal of forensic sciences, 46 5
P. Hebert, S. Ratnasingham, Jeremy Waard (2003)
Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related speciesProceedings of the Royal Society of London. Series B: Biological Sciences, 270
Wells J. D. Pape T. Sperling F. A. H.
DNA-based identification and molecular systematics of forensically important
T. Pape, S. McKillup, R. Mckillup (2000)
Two new species of Sarcophaga (Sarcorohdendorfia) Baranov (Diptera: Sarcophagidae), parasitoids of Littoraria filosa (Sowerby) (Gastropoda: Littorinidae)Australian Journal of Entomology, 39
T. Pape (1996)
Catalogue of the Sarcophagidae of the world (Insecta:Diptera)
Tamura K. Dudley J. Nei M. Kumar S.
MEGA4: Molecular Evolutionary Genetics
Petra Boehme, J. Amendt, R. Disney, R. Zehner (2010)
Molecular identification of carrion-breeding scuttle flies (Diptera: Phoridae) using COI barcodesInternational Journal of Legal Medicine, 124
M. Kimura (1980)
A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequencesJournal of Molecular Evolution, 16
Carrion-breeding Sarcophagidae (Diptera) can be used to estimate the post-mortem interval in forensic cases. Difficulties with accurate morphological identifications at any life stage and a lack of documented thermobiological profiles have limited their current usefulness. The molecular-based approach of DNA barcoding, which utilises a 648-bp fragment of the mitochondrial cytochrome oxidase subunit I gene, was evaluated in a pilot study for discrimination between 16 Australian sarcophagids. The current study comprehensively evaluated barcoding for a larger taxon set of 588 Australian sarcophagids. In total, 39 of the 84 known Australian species were represented by 580 specimens, which includes 92% of potentially forensically important species. A further eight specimens could not be identified, but were included nonetheless as six unidentifiable taxa. A neighbour-joining tree was generated and nucleotide sequence divergences were calculated. All species except Sarcophaga ( Fergusonimyia ) bancroftorum , known for high morphological variability, were resolved as monophyletic (99.2% of cases), with bootstrap support of 100. Excluding S. bancroftorum , the mean intraspecific and interspecific variation ranged from 1.12% and 2.81–11.23%, respectively, allowing for species discrimination. DNA barcoding was therefore validated as a suitable method for molecular identification of Australian Sarcophagidae, which will aid in the implementation of this fauna in forensic entomology.
Invertebrate Systematics – CSIRO Publishing
Published: Dec 19, 2012
Keywords: COI, forensic entomology.
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.