Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Microcalorimetry of Biological MoleculesEnzyme Kinetics Determined by Single-Injection Isothermal Titration Calorimetry

Microcalorimetry of Biological Molecules: Enzyme Kinetics Determined by Single-Injection... [This chapter describes how to collect Michaelis-Menten kinetic data on an enzyme-catalyzed reaction with the isothermal titration calorimetry (ITC) and the single-injection method. ITC measures the heat rate which is directly proportional to the reaction rate. The enthalpy change (ΔrH), Km, kcat, and vmax are determined in a single assay that does not require labeling or immobilization.] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

Microcalorimetry of Biological MoleculesEnzyme Kinetics Determined by Single-Injection Isothermal Titration Calorimetry

Part of the Methods in Molecular Biology Book Series (volume 1964)
Editors: Ennifar, Eric

Loading next page...
 
/lp/springer-journals/microcalorimetry-of-biological-molecules-enzyme-kinetics-determined-by-1OjU6YeuCv

References (7)

Publisher
Springer New York
Copyright
© Springer Science+Business Media, LLC, part of Springer Nature 2019
ISBN
978-1-4939-9178-5
Pages
241 –249
DOI
10.1007/978-1-4939-9179-2_17
Publisher site
See Chapter on Publisher Site

Abstract

[This chapter describes how to collect Michaelis-Menten kinetic data on an enzyme-catalyzed reaction with the isothermal titration calorimetry (ITC) and the single-injection method. ITC measures the heat rate which is directly proportional to the reaction rate. The enthalpy change (ΔrH), Km, kcat, and vmax are determined in a single assay that does not require labeling or immobilization.]

Published: Mar 31, 2019

Keywords: Kinetics; ITC; Michaelis-Menten; Enzyme; Single-injection method; Calorimetry; Enthalpy

There are no references for this article.