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[This chapter describes how to collect Michaelis-Menten kinetic data on an enzyme-catalyzed reaction with the isothermal titration calorimetry (ITC) and the single-injection method. ITC measures the heat rate which is directly proportional to the reaction rate. The enthalpy change (ΔrH), Km, kcat, and vmax are determined in a single assay that does not require labeling or immobilization.]
Published: Mar 31, 2019
Keywords: Kinetics; ITC; Michaelis-Menten; Enzyme; Single-injection method; Calorimetry; Enthalpy
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