Access the full text.
Sign up today, get DeepDyve free for 14 days.
I. Hidalgo, Thomas Raub, R. Borchardt (1989)
Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability.Gastroenterology, 96 3
C. Legrand, J. Bour, C. Jacob, J. Capiaumont, A. Martial, A. Marc, M. Wudtke, G. Kretzmer, C. Demangel, D. Duval, J. Hache (1992)
Lactate dehydrogenase (LDH) activity of the number of dead cells in the medium of cultured eukaryotic cells as markerJournal of Biotechnology, 25
S. Murphy, A. Atala (2014)
3D bioprinting of tissues and organsNature Biotechnology, 32
K. Foster, C. Oster, Mary Mayer, M. Avery, K. Audus (1998)
Characterization of the A549 cell line as a type II pulmonary epithelial cell model for drug metabolism.Experimental cell research, 243 2
Lenke Horváth, Y. Umehara, Corinne Jud, F. Blank, A. Petri‐Fink, B. Rothen‐Rutishauser (2015)
Engineering an in vitro air-blood barrier by 3D bioprintingScientific Reports, 5
P. Artursson, J. Karlsson (1991)
Correlation between oral drug absorption in humans and apparent drug permeability coefficients in human intestinal epithelial (Caco-2) cells.Biochemical and biophysical research communications, 175 3
A. Astashkina, B. Mann, D. Grainger (2012)
A critical evaluation of in vitro cell culture models for high-throughput drug screening and toxicity.Pharmacology & therapeutics, 134 1
T. Hartung, C. Rovida (2009)
Chemical regulators have overreachedNature, 460
Y. Sambuy, I. Angelis, G. Ranaldi, M. Scarino, A. Stammati, F. Zucco (2005)
The Caco-2 cell line as a model of the intestinal barrier: influence of cell and culture-related factors on Caco-2 cell functional characteristicsCell Biology and Toxicology, 21
H. Wan, H. Winton, C. Soeller, Geoffrey Stewart, P. Thompson, Dieter Gruenert, M. Cannell, D. Garrod, Clive Robinson (2000)
Tight junction properties of the immortalized human bronchial epithelial cell lines Calu-3 and 16HBE14o-.The European respiratory journal, 15 6
M. Lieber, G. Todaro, Barry Smith, A. Szakal, W. Nelson-Rees (1976)
A continuous tumor‐cell line from a human lung carcinoma with properties of type II alveolar epithelial cellsInternational Journal of Cancer, 17
M. Xia, Ruili Huang, K. Witt, Noel Southall, J. Fostel, Ming-Hsuang Cho, A. Jadhav, Cynthia Smith, James Inglese, C. Portier, R. Tice, C. Austin (2007)
Compound Cytotoxicity Profiling Using Quantitative High-Throughput ScreeningEnvironmental Health Perspectives, 116
P. Fratzl (2008)
Collagen: Structure and Mechanics, an Introduction
Nan Li, Yanfeng Liu, J. Qiu, Lingyan Zhong, N. Alépée, J. Cotovio, Zhenzi Cai (2017)
In vitro skin irritation assessment becomes a reality in China using a reconstructed human epidermis test method.Toxicology in vitro : an international journal published in association with BIBRA, 41
Donald Shapiro, L. Nardone, S. Rooney, Etsuro Motoyama, Jose Munoz (1978)
Phospholipid biosynthesis and secretion by a cell line (A549) which resembles type II aleveolar epithelial cells.Biochimica et biophysica acta, 530 2
A. Cozens, M. Yezzi, K. Kunzelmann, T. Ohrui, Leslie Chin, K. Eng, W. Finkbeiner, J. Widdicombe, D. Gruenert (1994)
CFTR expression and chloride secretion in polarized immortal human bronchial epithelial cells.American journal of respiratory cell and molecular biology, 10 1
B. Forbes, C. Ehrhardt (2005)
Human respiratory epithelial cell culture for drug delivery applications.European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 60 2
M. Balda, J. Whitney, Catalina Flores, Sirenia Gonz~ilez, M. Cereijido, K. Matter (1996)
Functional dissociation of paracellular permeability and transepithelial electrical resistance and disruption of the apical- basolateral intramembrane diffusion barrier by expression of a mutant tight junction membrane proteinThe Journal of Cell Biology, 134
Sunita Shukla, Ruili Huang, C. Austin, M. Xia (2010)
The future of toxicity testing: a focus on in vitro methods using a quantitative high-throughput screening platform.Drug discovery today, 15 23-24
Rasmus Foldbjerg, D. Dang, H. Autrup (2011)
Cytotoxicity and genotoxicity of silver nanoparticles in the human lung cancer cell line, A549Archives of Toxicology, 85
Falguni Pati, Jinah Jang, Dong-Heon Ha, Sung Kim, J. Rhie, J. Shim, Deok‐Ho Kim, D. Cho (2014)
Printing three-dimensional tissue analogues with decellularized extracellular matrix bioinkNature Communications, 5
S. Stern, S. McNeil (2008)
Nanotechnology safety concerns revisited.Toxicological sciences : an official journal of the Society of Toxicology, 101 1
B. Williams, R. Gelman, D. Poppke, K. Piez (1978)
Collagen fibril formation. Optimal in vitro conditions and preliminary kinetic results.The Journal of biological chemistry, 253 18
P. Fratzl (2008)
Collagen : structure and mechanics
Silke Wüst, M. Godla, R. Müller, S. Hofmann (2014)
Tunable hydrogel composite with two-step processing in combination with innovative hardware upgrade for cell-based three-dimensional bioprinting.Acta biomaterialia, 10 2
D. Velegol, F. Lanni (2001)
Cell traction forces on soft biomaterials. I. Microrheology of type I collagen gels.Biophysical journal, 81 3
[Increasing ethical and biological concerns require a paradigm shift toward animal-free testing strategies for drug testing and hazard assessments. To this end, the application of bioprinting technology in the field of biomedicine is driving a rapid progress in tissue engineering. In particular, standardized and reproducible in vitro models produced by three-dimensional (3D) bioprinting technique represent a possible alternative to animal models, enabling in vitro studies relevant to in vivo conditions. The innovative approach of 3D bioprinting allows a spatially controlled deposition of cells and biomaterial in a layer-by-layer fashion providing a platform for engineering reproducible models. However, despite the promising and revolutionizing character of 3D bioprinting technology, standardized protocols providing detailed instructions are lacking. Here, we provide a protocol for the automatized printing of simple alveolar, bronchial, and intestine epithelial cell layers as the basis for more complex respiratory and gastrointestinal tissue models. Such systems will be useful for high-throughput toxicity screening and drug efficacy evaluation.]
Published: Mar 24, 2020
Keywords: In vitro cultures; Alveolar epithelial cells; Bronchial epithelial cells; Intestine epithelial cells; Bioprinting technique
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.