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A SIMPLIFIED METHOD OF STAINING BACTERIA, CAPSULATED BACTERIA IN BODY FLUIDS, AND PREPARATIONS FOR OPSONIC COUNTS

A SIMPLIFIED METHOD OF STAINING BACTERIA, CAPSULATED BACTERIA IN BODY FLUIDS, AND PREPARATIONS... For over four years I have used with great satisfaction the following method for the staining of bacteria and body fluids in place of Löffler's methylen-blue, in the staining of preparations for the opsonic count instead of Leishman's or Wright's blood-stain, and in the staining of capsulated bacteria. I describe it here in the belief that it may be of help to clinicians as well as to laboratory workers. PREPARATION OF STAINING SOLUTIONS The preparation of this stain requires no saturated alcoholic solutions, nor the troublesome "1 to 10,000" aqueous solution of caustic potash necessary in the preparation of Löffler's methylen-blue solution. It is easily prepared in a few minutes by any physician or druggist and keeps indefinitely. The addition of a few crystals of sodium carbonate may be necessary from time to time to counteract the acidity of the corrosive sublimate which is used, as a fixative. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png JAMA American Medical Association

A SIMPLIFIED METHOD OF STAINING BACTERIA, CAPSULATED BACTERIA IN BODY FLUIDS, AND PREPARATIONS FOR OPSONIC COUNTS

JAMA , Volume LVI (16) – Apr 22, 1911

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Publisher
American Medical Association
Copyright
Copyright © 1911 American Medical Association. All Rights Reserved. Applicable FARS/DFARS Restrictions Apply to Government Use.
ISSN
0098-7484
eISSN
1538-3598
DOI
10.1001/jama.1911.02560160031009
Publisher site
See Article on Publisher Site

Abstract

For over four years I have used with great satisfaction the following method for the staining of bacteria and body fluids in place of Löffler's methylen-blue, in the staining of preparations for the opsonic count instead of Leishman's or Wright's blood-stain, and in the staining of capsulated bacteria. I describe it here in the belief that it may be of help to clinicians as well as to laboratory workers. PREPARATION OF STAINING SOLUTIONS The preparation of this stain requires no saturated alcoholic solutions, nor the troublesome "1 to 10,000" aqueous solution of caustic potash necessary in the preparation of Löffler's methylen-blue solution. It is easily prepared in a few minutes by any physician or druggist and keeps indefinitely. The addition of a few crystals of sodium carbonate may be necessary from time to time to counteract the acidity of the corrosive sublimate which is used, as a fixative.

Journal

JAMAAmerican Medical Association

Published: Apr 22, 1911

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