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Multiple-Organ Failure and Sepsis Without Bacteria: An Experimental Model

Multiple-Organ Failure and Sepsis Without Bacteria: An Experimental Model Abstract • Multiple-organ failure is generally attributed to bacterial infection, although a correlation with positive blood cultures is not consistently found. Consequently, we studied the effects of a local nonbacterial inflammatory stimulus on distant organ functions and metabolism. Wistar rats were inoculated intraperitoneally with zymosan. Heart and ventilatory rates, oxygen consumption, and body temperature were measured. Survivors were killed at day 12 for blood analysis, weighing of organs, and microscopy. Intraperitoneal zymosan resulted in an early hyperdynamic "septic" response with a 35% mortality. After a few days, oxygen consumption decreased, serum lactate levels increased, and the function of multiple organs deteriorated, while blood cultures remained sterile. The experiment was repeated in germ-free rats with similar results but a lower mortality. We concluded that a severe inflammatory response in itself is capable of inducing multiple-organ failure with "sepsis." (Arch Surg 1986;121:897-901) References 1. Ashbaugh DG, Petty TL: Sepsis complicating the acute respiratory distress syndrome . Surg Gynecol Obstet 1972;135:865-868. 2. Goris RJA, teBoekhorst T, Nuytinck JKS: Multiple organ failure: Generalized autodestructive inflammation? Arch Surg 1985;120:1109-1115.Crossref 3. Wichterman KA, Baue AE, Chaudry IH: Sepsis and septic shock: A review of laboratory models and a proposal . J Surg Res 1980;29:189-201.Crossref 4. Lang CH, Bagby GJ, Bornside GH, et al: Sustained hypermetabolic sepsis in rats: Characterization of the model . J Surg Res 1983;35:201-210.Crossref 5. Oh GR, Mela-Riker LM, Bryant RE, et al: A new experimental model of chronic high-output sepsis . Circ Shock 1984;13:99. 6. Kirton OC, Jones R, Zapol WM, et al: The development of a model of subacute lung injury after intra-abdominal infection . Surgery 1984;96:384-394. 7. Joyce LD, Hau T, Hoffman R, et al: Evaluation of the mechanism of zymosan-induced resistance to experimental peritonitis . Surgery 1978;83: 717-725. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Surgery American Medical Association

Multiple-Organ Failure and Sepsis Without Bacteria: An Experimental Model

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References (7)

Publisher
American Medical Association
Copyright
Copyright © 1986 American Medical Association. All Rights Reserved.
ISSN
0004-0010
eISSN
1538-3644
DOI
10.1001/archsurg.1986.01400080039006
Publisher site
See Article on Publisher Site

Abstract

Abstract • Multiple-organ failure is generally attributed to bacterial infection, although a correlation with positive blood cultures is not consistently found. Consequently, we studied the effects of a local nonbacterial inflammatory stimulus on distant organ functions and metabolism. Wistar rats were inoculated intraperitoneally with zymosan. Heart and ventilatory rates, oxygen consumption, and body temperature were measured. Survivors were killed at day 12 for blood analysis, weighing of organs, and microscopy. Intraperitoneal zymosan resulted in an early hyperdynamic "septic" response with a 35% mortality. After a few days, oxygen consumption decreased, serum lactate levels increased, and the function of multiple organs deteriorated, while blood cultures remained sterile. The experiment was repeated in germ-free rats with similar results but a lower mortality. We concluded that a severe inflammatory response in itself is capable of inducing multiple-organ failure with "sepsis." (Arch Surg 1986;121:897-901) References 1. Ashbaugh DG, Petty TL: Sepsis complicating the acute respiratory distress syndrome . Surg Gynecol Obstet 1972;135:865-868. 2. Goris RJA, teBoekhorst T, Nuytinck JKS: Multiple organ failure: Generalized autodestructive inflammation? Arch Surg 1985;120:1109-1115.Crossref 3. Wichterman KA, Baue AE, Chaudry IH: Sepsis and septic shock: A review of laboratory models and a proposal . J Surg Res 1980;29:189-201.Crossref 4. Lang CH, Bagby GJ, Bornside GH, et al: Sustained hypermetabolic sepsis in rats: Characterization of the model . J Surg Res 1983;35:201-210.Crossref 5. Oh GR, Mela-Riker LM, Bryant RE, et al: A new experimental model of chronic high-output sepsis . Circ Shock 1984;13:99. 6. Kirton OC, Jones R, Zapol WM, et al: The development of a model of subacute lung injury after intra-abdominal infection . Surgery 1984;96:384-394. 7. Joyce LD, Hau T, Hoffman R, et al: Evaluation of the mechanism of zymosan-induced resistance to experimental peritonitis . Surgery 1978;83: 717-725.

Journal

Archives of SurgeryAmerican Medical Association

Published: Aug 1, 1986

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