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Effect of Low—Power Density Laser Radiation on Healing of Open Skin Wounds in Rats

Effect of Low—Power Density Laser Radiation on Healing of Open Skin Wounds in Rats Abstract • We performed a study to determine whether laser radiation of low-power density would affect the healing of open skin wounds in rats. The wounds were irradiated daily with a helium-neon laser (wavelength, 632.8 nm) and an argon laser (wavelength, 514.5 nm) at a constant power density of 45 mW/sq cm. The rate of wound closure was followed by photographing the wounds in a standardized way. The collagen hydroxyproline concentration in the scar tissue was determined on the 18th postoperative day. Helium-neon laser radiation had a statistically significant stimulating effect on collagen synthesis in the wound, with a maximum effect at an energy density of 4 joules/sq cm. The rate of wound closure was enhanced significantly between the third and 12th postoperative days. The argon laser exposure produced a significant increase in collagen concentration both in irradiated and nonirradiated contralateral wounds. However, an acceleration of the healing rate was not registered in this case. The wound contraction up to the fourth day of the experiment was inhibited under helium-neon and argon laser exposure to 20 joules/sq cm. The described effects were not specific for the laser light. There may be a wavelength-selective influence of coherent light on the metabolic and proliferation processes in wound healing, with the associated problem of the possible carcinogenic effects of laser radiation. (Arch Surg 1981;116:293-296) References 1. Hug DH: The activation of enzymes with light , in Smith KC (ed): Photochemical and Photobiological Reviews . New York, Plenum Press, 1978, vol 3, pp 1-33. 2. Kollias N, Melander WR: Laser induced stimulation of chymotrypsin activity . Phys Lett A 1976;57:102-104.Crossref 3. Carney SA, Lawrence JC, Ricketts CR: The effect of light of a ruby laser on the metabolism of skin in tissue culture . Biochim Biophys Acta 1967;148:525-530.Crossref 4. Mester E, Varterèsz V, Doeklen A, et al: Die Wirkung der Laserstrahlen auf die Haemoglobinsynthese in vitro . Radiobiol Radiother 1971;12:669-675. 5. Mester E, Jászági-Nagy E: The effect of laser radiation on wound healing and collagen synthesis . Stud Biophys 1973;35:227-230. 6. Srivastava AS, De SK: The influence of different frequencies of laser on the microbial production of alcohol by Saccharomyces cerevisae . J Indian Chem Soc 1978;55:203-205. 7. Hardy LB, Hardy FS, Fine S, et al: Effect of ruby laser radiation on mouse fibroblast culture . Fed Proc 1967;26:668. 8. Mester E, Nagy EJ, Báscy E, et al: Laser in der Medizin—experimentelle und klinische Erfahrungen , in Kaul A (ed): Medizinische Physik in Forschung und Praxis . Berlin, Walter de Gruyter, 1976, pp 117-135. 9. Moskalik KG, Kozlov AP, Lazo VV, et al: Stimulation of cell proliferation by pulsed laser radiation . Dokl Akad Nauk SSSR 1977;233:695-697. 10. Stepanov BI, Mostovnikov VA, Rubinov AN, et al: Regulation of the functional activity of human cells with laser radiation . Dokl Akad Nauk SSSR 1977;236:1007-1010. 11. Moskalik KG, Kniazev PG, Lazo VV: Radioautographic study of fibroblast culture after neodymium laser irradiation . Arkh Anat Gistol Embriol 1979;76( (3) ):28-34. 12. Shakhtmeister IY, Valtseva IA, Shekhter AB, et al: Biologic reactions of the body to the application of a laser beam to the skin . Vestn Dermatol Venerol 1974;50( (12) ):7-12. 13. Zadorozhnyi BA, Safronov BG, Dyuba VM: Morphological and histochemical changes in the skin under the influence of low-power gas lasers . Vestn Dermatol Venerol 1976;52( (8) ):34-39. 14. Mester E, Spiry T, Szende B, et al: Effect of laser rays on wound healing . Am J Surg 1971;122:532-535.Crossref 15. Vejrosta Z, Luklová L, Hornová J, et al: Laser radiation in healing oral cavity wounds . Scr Med 1975;48:569-576. 16. Averbakh MM, Sorkin MZ, Dobkin VG, et al: Effect of helium-neon laser on the healing of aseptic experimental wounds . Eksp Khir Anesteziol 1976;3:56-59. 17. Babayan R, Farthmann F: Laser und Wundheilung . Zentralbl Chir 1977;102:1247-1250. 18. Hutschenreiter G, Haina D, Paulini K, et al: Wundheilung nach Laserund Rotlichtbestrahlung. Z Exp Chir. to be published. 19. Stegemann H: Mikrobestimmung von Hydroxyprolin mit Chloramin-T und p-Dimethylaminobenzaldehyd . Hoppe Seylers Z Physiol Chem 1958;311:41-45.Crossref 20. McGuff PE, Deterling RA Jr, Gottlieb LS, et al: Effects of laser radiation on tumor transplants . Fed Proc 1965;24( (suppl 14) ):150-154. 21. Tomson SH, Emmett EA, Fox SH: Photodestruction of mouse epithelial tumors after oral acridine orange and argon laser . Cancer Res 1974;34:3124-3127. 22. Urazalin ZB, Antipova ZP: Effect of monochromatic red light on mandibular fracture healing . Stomatologiia 1978;57( (5) ):5-9. 23. Mester E, Nagylucskay S, Tisza S, et al: Stimulation of wound healing by means of laser rays: III. Investigation of the effect on immune competent cells . Acta Chir Acad Sci Hung 1978;19:163-170. 24. Mester E, Nagylucskay S, Waidelich W, et al: Auswirkungen direkter Laserbestrahlung auf menschliche Lymphocyten . Arch Dermatol Res 1978;263:241-245.Crossref 25. Goldman L, Richfield DF: The effect of repeated exposures to laser beams: Case report with nine months' period of observation . Acta Derm Venereol 1964;44:264-268. 26. Goldman L: Biomedical Aspects of the Laser: The Introduction of Laser Applications Into Biology and Medicine . Berlin, Springer Verlag, 1967. 27. Parr WH, Fisher RS: Aberrant corneal epithelial cells produced by ruby laser irradiation . Invest Ophthalmol 1967;6:356-363. 28. Mester E, Spiry T, Szende B: Effect of laser rays on wound healing . Bull Soc Int Chir 1973;32:169-173. 29. Rounds DE, Chamberlain EC, Okigaki T: Laser radiation of tissue culture . Ann NY Acad Sci 1965;122:713-727.Crossref 30. Gordon TE, Bishop K, Parsons RL, et al: Laser effect on oral hard and soft tissues . Lasers Optiques Non Conventionelle 1967;8:102-106. 31. Moskalik KG, Pertsov OL: On cytogenetic effects of neodymium laser . Tsitologiia 1974;16:1284-1288. 32. Rabkin BM,Tarasov VA: Cytogenetic effect of laser radiation of wavelength 6328 Å in sprouts of Allium fistulosum L . Dokl Akad Nauk SSSR 1968;180:1471-1472. 33. Belcheva RG, Dimcheva-Grozdanova LV: Chromosome aberrations and malformations in Salmo irideus Gibb after the irradiation of early-cleavage embryos with lasers of different wave-length . Dokl Bolg Akad Nauk 1973;26:1077-1080. 34. Mester E, Sellyea M, Tota GJ: Laserstrahlenwirkung auf das Wachstum des Ehrlichschen Aszitestumors: Vorlaeufige Mitteilung . Arch Geschwulstforsch 1968;32:201-206. 35. Mims JL: Some effects of low energy ruby and neodymium laser radiation on transplantable subcutaneous mouse tumors . Tex Rep Biol Med 1968;26:434-435. 36. Jamieson CW, Litwin MS, Longo SE, et al: Enhancement of melanoma cell culture growth rate by ruby laser radiation . Life Sci 1969;8:101-106.Crossref 37. Skibenko KP, Tsutsaeva AA, Dudnich LA: Investigation on the effect of low power laser radiation on Ehrlich adenocarcinoma cells , in Cryobiology and Cryomedicine . Kiev, USSR, Znanie, 1976, vol 2, pp 91-94. 38. Moskalik KG, Kozlov AP: Effect of pulsed laser radiation on mitotic activity and DNA synthesis of tumor cells . Dokl Akad Nauk SSSR 1979;244:206-208. 39. List RJ (ed): Smithsonian Meteorological Tables , ed 6. Washington, DC, Smithsonian Institution, 1951, pp 416, 431. 40. Mester E, Kosma L, Dudash V, et al: The effect of laser radiation on the phagocytic activity of leucocytes . Dokl Akad Nauk BSSR 1979;23:749-752. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Surgery American Medical Association

Effect of Low—Power Density Laser Radiation on Healing of Open Skin Wounds in Rats

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References (48)

Publisher
American Medical Association
Copyright
Copyright © 1981 American Medical Association. All Rights Reserved.
ISSN
0004-0010
eISSN
1538-3644
DOI
10.1001/archsurg.1981.01380150021005
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Abstract

Abstract • We performed a study to determine whether laser radiation of low-power density would affect the healing of open skin wounds in rats. The wounds were irradiated daily with a helium-neon laser (wavelength, 632.8 nm) and an argon laser (wavelength, 514.5 nm) at a constant power density of 45 mW/sq cm. The rate of wound closure was followed by photographing the wounds in a standardized way. The collagen hydroxyproline concentration in the scar tissue was determined on the 18th postoperative day. Helium-neon laser radiation had a statistically significant stimulating effect on collagen synthesis in the wound, with a maximum effect at an energy density of 4 joules/sq cm. The rate of wound closure was enhanced significantly between the third and 12th postoperative days. The argon laser exposure produced a significant increase in collagen concentration both in irradiated and nonirradiated contralateral wounds. However, an acceleration of the healing rate was not registered in this case. The wound contraction up to the fourth day of the experiment was inhibited under helium-neon and argon laser exposure to 20 joules/sq cm. The described effects were not specific for the laser light. There may be a wavelength-selective influence of coherent light on the metabolic and proliferation processes in wound healing, with the associated problem of the possible carcinogenic effects of laser radiation. (Arch Surg 1981;116:293-296) References 1. Hug DH: The activation of enzymes with light , in Smith KC (ed): Photochemical and Photobiological Reviews . New York, Plenum Press, 1978, vol 3, pp 1-33. 2. Kollias N, Melander WR: Laser induced stimulation of chymotrypsin activity . Phys Lett A 1976;57:102-104.Crossref 3. Carney SA, Lawrence JC, Ricketts CR: The effect of light of a ruby laser on the metabolism of skin in tissue culture . Biochim Biophys Acta 1967;148:525-530.Crossref 4. Mester E, Varterèsz V, Doeklen A, et al: Die Wirkung der Laserstrahlen auf die Haemoglobinsynthese in vitro . Radiobiol Radiother 1971;12:669-675. 5. Mester E, Jászági-Nagy E: The effect of laser radiation on wound healing and collagen synthesis . Stud Biophys 1973;35:227-230. 6. Srivastava AS, De SK: The influence of different frequencies of laser on the microbial production of alcohol by Saccharomyces cerevisae . J Indian Chem Soc 1978;55:203-205. 7. Hardy LB, Hardy FS, Fine S, et al: Effect of ruby laser radiation on mouse fibroblast culture . Fed Proc 1967;26:668. 8. Mester E, Nagy EJ, Báscy E, et al: Laser in der Medizin—experimentelle und klinische Erfahrungen , in Kaul A (ed): Medizinische Physik in Forschung und Praxis . Berlin, Walter de Gruyter, 1976, pp 117-135. 9. Moskalik KG, Kozlov AP, Lazo VV, et al: Stimulation of cell proliferation by pulsed laser radiation . Dokl Akad Nauk SSSR 1977;233:695-697. 10. Stepanov BI, Mostovnikov VA, Rubinov AN, et al: Regulation of the functional activity of human cells with laser radiation . Dokl Akad Nauk SSSR 1977;236:1007-1010. 11. Moskalik KG, Kniazev PG, Lazo VV: Radioautographic study of fibroblast culture after neodymium laser irradiation . Arkh Anat Gistol Embriol 1979;76( (3) ):28-34. 12. Shakhtmeister IY, Valtseva IA, Shekhter AB, et al: Biologic reactions of the body to the application of a laser beam to the skin . Vestn Dermatol Venerol 1974;50( (12) ):7-12. 13. Zadorozhnyi BA, Safronov BG, Dyuba VM: Morphological and histochemical changes in the skin under the influence of low-power gas lasers . Vestn Dermatol Venerol 1976;52( (8) ):34-39. 14. Mester E, Spiry T, Szende B, et al: Effect of laser rays on wound healing . Am J Surg 1971;122:532-535.Crossref 15. Vejrosta Z, Luklová L, Hornová J, et al: Laser radiation in healing oral cavity wounds . Scr Med 1975;48:569-576. 16. Averbakh MM, Sorkin MZ, Dobkin VG, et al: Effect of helium-neon laser on the healing of aseptic experimental wounds . Eksp Khir Anesteziol 1976;3:56-59. 17. Babayan R, Farthmann F: Laser und Wundheilung . Zentralbl Chir 1977;102:1247-1250. 18. Hutschenreiter G, Haina D, Paulini K, et al: Wundheilung nach Laserund Rotlichtbestrahlung. Z Exp Chir. to be published. 19. Stegemann H: Mikrobestimmung von Hydroxyprolin mit Chloramin-T und p-Dimethylaminobenzaldehyd . Hoppe Seylers Z Physiol Chem 1958;311:41-45.Crossref 20. McGuff PE, Deterling RA Jr, Gottlieb LS, et al: Effects of laser radiation on tumor transplants . Fed Proc 1965;24( (suppl 14) ):150-154. 21. Tomson SH, Emmett EA, Fox SH: Photodestruction of mouse epithelial tumors after oral acridine orange and argon laser . Cancer Res 1974;34:3124-3127. 22. Urazalin ZB, Antipova ZP: Effect of monochromatic red light on mandibular fracture healing . Stomatologiia 1978;57( (5) ):5-9. 23. Mester E, Nagylucskay S, Tisza S, et al: Stimulation of wound healing by means of laser rays: III. Investigation of the effect on immune competent cells . Acta Chir Acad Sci Hung 1978;19:163-170. 24. Mester E, Nagylucskay S, Waidelich W, et al: Auswirkungen direkter Laserbestrahlung auf menschliche Lymphocyten . Arch Dermatol Res 1978;263:241-245.Crossref 25. Goldman L, Richfield DF: The effect of repeated exposures to laser beams: Case report with nine months' period of observation . Acta Derm Venereol 1964;44:264-268. 26. Goldman L: Biomedical Aspects of the Laser: The Introduction of Laser Applications Into Biology and Medicine . Berlin, Springer Verlag, 1967. 27. Parr WH, Fisher RS: Aberrant corneal epithelial cells produced by ruby laser irradiation . Invest Ophthalmol 1967;6:356-363. 28. Mester E, Spiry T, Szende B: Effect of laser rays on wound healing . Bull Soc Int Chir 1973;32:169-173. 29. Rounds DE, Chamberlain EC, Okigaki T: Laser radiation of tissue culture . Ann NY Acad Sci 1965;122:713-727.Crossref 30. Gordon TE, Bishop K, Parsons RL, et al: Laser effect on oral hard and soft tissues . Lasers Optiques Non Conventionelle 1967;8:102-106. 31. Moskalik KG, Pertsov OL: On cytogenetic effects of neodymium laser . Tsitologiia 1974;16:1284-1288. 32. Rabkin BM,Tarasov VA: Cytogenetic effect of laser radiation of wavelength 6328 Å in sprouts of Allium fistulosum L . Dokl Akad Nauk SSSR 1968;180:1471-1472. 33. Belcheva RG, Dimcheva-Grozdanova LV: Chromosome aberrations and malformations in Salmo irideus Gibb after the irradiation of early-cleavage embryos with lasers of different wave-length . Dokl Bolg Akad Nauk 1973;26:1077-1080. 34. Mester E, Sellyea M, Tota GJ: Laserstrahlenwirkung auf das Wachstum des Ehrlichschen Aszitestumors: Vorlaeufige Mitteilung . Arch Geschwulstforsch 1968;32:201-206. 35. Mims JL: Some effects of low energy ruby and neodymium laser radiation on transplantable subcutaneous mouse tumors . Tex Rep Biol Med 1968;26:434-435. 36. Jamieson CW, Litwin MS, Longo SE, et al: Enhancement of melanoma cell culture growth rate by ruby laser radiation . Life Sci 1969;8:101-106.Crossref 37. Skibenko KP, Tsutsaeva AA, Dudnich LA: Investigation on the effect of low power laser radiation on Ehrlich adenocarcinoma cells , in Cryobiology and Cryomedicine . Kiev, USSR, Znanie, 1976, vol 2, pp 91-94. 38. Moskalik KG, Kozlov AP: Effect of pulsed laser radiation on mitotic activity and DNA synthesis of tumor cells . Dokl Akad Nauk SSSR 1979;244:206-208. 39. List RJ (ed): Smithsonian Meteorological Tables , ed 6. Washington, DC, Smithsonian Institution, 1951, pp 416, 431. 40. Mester E, Kosma L, Dudash V, et al: The effect of laser radiation on the phagocytic activity of leucocytes . Dokl Akad Nauk BSSR 1979;23:749-752.

Journal

Archives of Surgery – American Medical Association

Published: Mar 1, 1981

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