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Seeding Endothelium Onto Canine Arterial Prostheses: The Effects of Graft Design

Seeding Endothelium Onto Canine Arterial Prostheses: The Effects of Graft Design Abstract • To identify prosthetic characteristics that support the proliferation of an endothelial lining, 14 different graft designs were studied in dogs. The grafts were prepared by the use of a technique of endothelial seeding that we described previously. They were studied two and four weeks after their implantation. Weft-knit Dacron grafts (water porosity from 1,400 to 1,650 mL/min/sq cm) were the most successful. A velour index (velour stitch frequency times velour loop height) was computed. When the internal velour index (IVI) was >60,000 loop-μ/sq cm, the percentage of clot-free surface was 17.2 ± 14.5%, compared with 62.0 ± 26.9% when the IVI <60,000 loop-μ/sq cm. Endothelium could be detected on 61.5% of the grafts when the IVI <60,000 loop-μ/sq cm, compared with 0% when the IVI > 60,000 loop-μ/sq cm. The cells that formed the outer capsule were better organized and more adherent to the graft when an external velour was present, but external velour did not affect the inner lining. We conclude that weft-knit Dacron grafts with water porosities from 1,400 to 1,650 mL/min/sq cm and with limited internal velour (IVI < 60,000 loop-μ/sq cm) are suitable substrates for single-staged endothelial-cell seeding. (Arch Surg 114:679-682, 1979) References 1. Moore WS, Rosson CT, Hall AD, et al: A cause of infection in prosthetic vascular grafts . Am J Surg 117:342-343, 1969.Crossref 2. Herring MB, Gardner AL, Glover J: A single-staged technique for seeding vascular grafts with autogenous endothelium . Surgery 84:498-504, 1978. 3. Sacks SA, Petritsch PH, Kaufman JJ: Canine kidney preservation using a new perfusate . Lancet 1:1024-1028, 1973.Crossref 4. Pugatch EMJ, Saunders AM: A new technique for making Haütchen preparations of unfixed aortic endothelium . J Atherosclerosis 8:735-738, 1968.Crossref 5. Wesolowski SA, Fries CC, Martinez A, et al: Arterial prosthetic materials . Ann NY Acad Sci 146:325-344, 1978.Crossref 6. Wesolowski SA: Performance of materials as prosthetic blood vessels . Bull NY Acad Med 48:331-356, 1972. 7. Berger K, Sauvage LR, Rao AM, et al: Healing of arterial prosthesis in man: Its incompleteness . Ann Surg 175:118-127, 1972.Crossref 8. Sauvage LR, Berger KE, Wood SJ, et al: Interspecies healing of porous arterial prostheses . Arch Surg 109:698-705, 1974.Crossref 9. Campbell CD, Goldfarb D, Detton DD, et al: Expanded polytetrafluoroethylene as a small artery substitute . Trans Am Soc Artif Intern Organs 20:86-90, 1974. 10. Szilagyi DE: An elastic Dacron arterial substitute . Surg Clin North Am 39:1523-1538, 1959. 11. Sharp WV, Taylor BC: Experience with negatively charged polyurethane-backed velours . J Biomed Mater Res 1:75-81, 1971.Crossref 12. Ross RN, Wright JTM, Eskin S, et al: Oxidized Orlon as a blood interface . J Biomed Mater Res 7:583-594, 1976.Crossref 13. Mansfield PB, Wechezak AR, Sauvage LR: Preventing thrombus on arterial vascular surfaces: True endothelial cell linings . Trans Am Soc Artif Intern Organs 21:264-272, 1975. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Surgery American Medical Association

Seeding Endothelium Onto Canine Arterial Prostheses: The Effects of Graft Design

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References (17)

Publisher
American Medical Association
Copyright
Copyright © 1979 American Medical Association. All Rights Reserved.
ISSN
0004-0010
eISSN
1538-3644
DOI
10.1001/archsurg.1979.01370300033004
Publisher site
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Abstract

Abstract • To identify prosthetic characteristics that support the proliferation of an endothelial lining, 14 different graft designs were studied in dogs. The grafts were prepared by the use of a technique of endothelial seeding that we described previously. They were studied two and four weeks after their implantation. Weft-knit Dacron grafts (water porosity from 1,400 to 1,650 mL/min/sq cm) were the most successful. A velour index (velour stitch frequency times velour loop height) was computed. When the internal velour index (IVI) was >60,000 loop-μ/sq cm, the percentage of clot-free surface was 17.2 ± 14.5%, compared with 62.0 ± 26.9% when the IVI <60,000 loop-μ/sq cm. Endothelium could be detected on 61.5% of the grafts when the IVI <60,000 loop-μ/sq cm, compared with 0% when the IVI > 60,000 loop-μ/sq cm. The cells that formed the outer capsule were better organized and more adherent to the graft when an external velour was present, but external velour did not affect the inner lining. We conclude that weft-knit Dacron grafts with water porosities from 1,400 to 1,650 mL/min/sq cm and with limited internal velour (IVI < 60,000 loop-μ/sq cm) are suitable substrates for single-staged endothelial-cell seeding. (Arch Surg 114:679-682, 1979) References 1. Moore WS, Rosson CT, Hall AD, et al: A cause of infection in prosthetic vascular grafts . Am J Surg 117:342-343, 1969.Crossref 2. Herring MB, Gardner AL, Glover J: A single-staged technique for seeding vascular grafts with autogenous endothelium . Surgery 84:498-504, 1978. 3. Sacks SA, Petritsch PH, Kaufman JJ: Canine kidney preservation using a new perfusate . Lancet 1:1024-1028, 1973.Crossref 4. Pugatch EMJ, Saunders AM: A new technique for making Haütchen preparations of unfixed aortic endothelium . J Atherosclerosis 8:735-738, 1968.Crossref 5. Wesolowski SA, Fries CC, Martinez A, et al: Arterial prosthetic materials . Ann NY Acad Sci 146:325-344, 1978.Crossref 6. Wesolowski SA: Performance of materials as prosthetic blood vessels . Bull NY Acad Med 48:331-356, 1972. 7. Berger K, Sauvage LR, Rao AM, et al: Healing of arterial prosthesis in man: Its incompleteness . Ann Surg 175:118-127, 1972.Crossref 8. Sauvage LR, Berger KE, Wood SJ, et al: Interspecies healing of porous arterial prostheses . Arch Surg 109:698-705, 1974.Crossref 9. Campbell CD, Goldfarb D, Detton DD, et al: Expanded polytetrafluoroethylene as a small artery substitute . Trans Am Soc Artif Intern Organs 20:86-90, 1974. 10. Szilagyi DE: An elastic Dacron arterial substitute . Surg Clin North Am 39:1523-1538, 1959. 11. Sharp WV, Taylor BC: Experience with negatively charged polyurethane-backed velours . J Biomed Mater Res 1:75-81, 1971.Crossref 12. Ross RN, Wright JTM, Eskin S, et al: Oxidized Orlon as a blood interface . J Biomed Mater Res 7:583-594, 1976.Crossref 13. Mansfield PB, Wechezak AR, Sauvage LR: Preventing thrombus on arterial vascular surfaces: True endothelial cell linings . Trans Am Soc Artif Intern Organs 21:264-272, 1975.

Journal

Archives of Surgery – American Medical Association

Published: Jun 1, 1979

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