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AN EXPERIMENT WITH BROTH CULTURES OF STAPHYLOCOCCUS AUREUS AND GENTIAN VIOLET

AN EXPERIMENT WITH BROTH CULTURES OF STAPHYLOCOCCUS AUREUS AND GENTIAN VIOLET Abstract Following the lines of experimentation of Churchman1 in 1922, when he showed that a slight increase in temperature had a definite effect on the bacteriostatic power of gentian violet, and of Gatch, Trusler, and Owen2 in 1925, when they showed that gentian violet in 4 per cent dextrose was less toxic for rabbits than aqueous gentian violet, experiments were performed in test tubes and on agar plates to correlate these facts. Twenty-four hour broth cultures of Staphylococcus aureus containing about 9 cc. of fluid were used. Gentian violet was prepared first with distilled water as a medium and then with 4 per cent dextrose as a medium. Sufficient amounts of the gentian violet preparations were added to the broth cultures to make a 1: 10,000 dilution of the dye. A bath at 50 C. was used to give the increase in temperature. A series of six broth cultures References 1. Churchman, J. W.: Bull. Johns Hopkins Hosp. 33:277 ( (June) ) 1922. 2. Gatch, W. D.; Trusler, H. M., and Owen, J. E.: Treatment of General Septicemia by Gentian Violet and Mercurochrome-220 Soluble , J. A. M. A. 85: 894 ( (Sept. 19) ) 1925.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Surgery American Medical Association

AN EXPERIMENT WITH BROTH CULTURES OF STAPHYLOCOCCUS AUREUS AND GENTIAN VIOLET

SHANDS, A. R.
Archives of Surgery , Volume 16 (5) – May 1, 1928
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References (2)

Publisher
American Medical Association
Copyright
Copyright © 1928 American Medical Association. All Rights Reserved.
ISSN
0004-0010
eISSN
1538-3644
DOI
10.1001/archsurg.1928.01140050102007
Publisher site
See Article on Publisher Site

Abstract

Abstract Following the lines of experimentation of Churchman1 in 1922, when he showed that a slight increase in temperature had a definite effect on the bacteriostatic power of gentian violet, and of Gatch, Trusler, and Owen2 in 1925, when they showed that gentian violet in 4 per cent dextrose was less toxic for rabbits than aqueous gentian violet, experiments were performed in test tubes and on agar plates to correlate these facts. Twenty-four hour broth cultures of Staphylococcus aureus containing about 9 cc. of fluid were used. Gentian violet was prepared first with distilled water as a medium and then with 4 per cent dextrose as a medium. Sufficient amounts of the gentian violet preparations were added to the broth cultures to make a 1: 10,000 dilution of the dye. A bath at 50 C. was used to give the increase in temperature. A series of six broth cultures References 1. Churchman, J. W.: Bull. Johns Hopkins Hosp. 33:277 ( (June) ) 1922. 2. Gatch, W. D.; Trusler, H. M., and Owen, J. E.: Treatment of General Septicemia by Gentian Violet and Mercurochrome-220 Soluble , J. A. M. A. 85: 894 ( (Sept. 19) ) 1925.Crossref

Journal

Archives of Surgery – American Medical Association

Published: May 1, 1928

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