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Development of Antigen During the Course of Serum Hepatitis: Measurements by Electron Microscopy and by Immunodiffusion

Development of Antigen During the Course of Serum Hepatitis: Measurements by Electron Microscopy... Abstract Fifty-six serum samples from 18 cases of acute hepatitis associated with the Australia antigen have been studied. The antigen has been identified by Prince's microtechnique of immunodiffusion and by electron microscopy with minor modifications after sera centrifugation without addition of antiserum.1 After preliminary centrifugation of diluted serum in phosphate buffered saline at 4 C for 30 minutes at 3,000 rpm, the supernatant is centrifuged twice at 45,000 g for one hour. The pellet is stained with 2% phosphatidic acid at pH 6.5 and examined at a magnification of 60,000. Several blood samples in the course of the disease were obtained until the antigen which was titrated on a two-fold dilution of sera had disappeared from the blood. Three typical forms of Au antigen2-6 ie, the small spherical particle, the large double-coated particle, and the tubular forms (Fig 1) were seen by electron microscopy. Five typical cases with References 1. Viral hepatitis and tests for the Australia (hepatitis-associated) antigen and antibody . Bull WHO 42:957-992, 1970. 2. Bayer ME, Blumberg BS, Werner B: Particles associated with Australia antigen in the sera of patients with leukaemia, Down's syndrome, and hepatitis . Nature 218:1057-1059, 1968.Crossref 3. Almeida JD, Waterson AP: Immune complexes in hepatitis . Lancet 2:983-986, 1969.Crossref 4. Hirschman RJ, Shulman NR, Barker LF, et al: Virus-like particles in sera of patients with infectious and serum hepatitis . JAMA 208:1667-1670, 1969.Crossref 5. Barker LF, Smith KO, Gehle WD, et al: Some antigenic and physical properties of virus-like particles in sera of hepatitis patients . J Immun 102:1529-1532, 1969. 6. Dane DS, Cameron CH, Briggs M: Virus-like particles in serum of patients with Australia antigen associated hepatitis . Lancet 1:695-698, 1970.Crossref 7. Wright R: The Australia antigen in chronic active hepatitis . Vox Sang 19:320-326, 1970.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png American Journal of Diseases of Children American Medical Association

Development of Antigen During the Course of Serum Hepatitis: Measurements by Electron Microscopy and by Immunodiffusion

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References (8)

Publisher
American Medical Association
Copyright
Copyright © 1972 American Medical Association. All Rights Reserved.
ISSN
0002-922X
DOI
10.1001/archpedi.1972.02110100052020
Publisher site
See Article on Publisher Site

Abstract

Abstract Fifty-six serum samples from 18 cases of acute hepatitis associated with the Australia antigen have been studied. The antigen has been identified by Prince's microtechnique of immunodiffusion and by electron microscopy with minor modifications after sera centrifugation without addition of antiserum.1 After preliminary centrifugation of diluted serum in phosphate buffered saline at 4 C for 30 minutes at 3,000 rpm, the supernatant is centrifuged twice at 45,000 g for one hour. The pellet is stained with 2% phosphatidic acid at pH 6.5 and examined at a magnification of 60,000. Several blood samples in the course of the disease were obtained until the antigen which was titrated on a two-fold dilution of sera had disappeared from the blood. Three typical forms of Au antigen2-6 ie, the small spherical particle, the large double-coated particle, and the tubular forms (Fig 1) were seen by electron microscopy. Five typical cases with References 1. Viral hepatitis and tests for the Australia (hepatitis-associated) antigen and antibody . Bull WHO 42:957-992, 1970. 2. Bayer ME, Blumberg BS, Werner B: Particles associated with Australia antigen in the sera of patients with leukaemia, Down's syndrome, and hepatitis . Nature 218:1057-1059, 1968.Crossref 3. Almeida JD, Waterson AP: Immune complexes in hepatitis . Lancet 2:983-986, 1969.Crossref 4. Hirschman RJ, Shulman NR, Barker LF, et al: Virus-like particles in sera of patients with infectious and serum hepatitis . JAMA 208:1667-1670, 1969.Crossref 5. Barker LF, Smith KO, Gehle WD, et al: Some antigenic and physical properties of virus-like particles in sera of hepatitis patients . J Immun 102:1529-1532, 1969. 6. Dane DS, Cameron CH, Briggs M: Virus-like particles in serum of patients with Australia antigen associated hepatitis . Lancet 1:695-698, 1970.Crossref 7. Wright R: The Australia antigen in chronic active hepatitis . Vox Sang 19:320-326, 1970.Crossref

Journal

American Journal of Diseases of ChildrenAmerican Medical Association

Published: Apr 1, 1972

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