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METHODS OF USING DIPHTHERIA TOXIN IN THE SCHICK TEST AND OF CONTROLLING THE REACTION

METHODS OF USING DIPHTHERIA TOXIN IN THE SCHICK TEST AND OF CONTROLLING THE REACTION The practical value that the Schick reaction1 has acquired in the diagnosis of susceptibility or immunity to diphtheria makes it desirable to say a few words about the toxin used in the test, the overneutralized or the heated toxin used in the control test, about the technic of the test, and finally about the interpretation of the reactions. The diphtheria toxin for use in the Schick test consists of a broth culture of the diphtheria bacillus, which has been grown in the thermostat at 37 C. for six days. To kill the living organisms 10 parts of a 5 per cent. solution of phenol (carbolic acid) (0.5 per cent.) are then added, and the bacteria allowed to sediment by keeping the broth culture in the ice box during the following two or three days. The supernatant culture fluid is now passed through a Berkefeld filter, and the clear filtrate http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png American journal of diseases of children American Medical Association

METHODS OF USING DIPHTHERIA TOXIN IN THE SCHICK TEST AND OF CONTROLLING THE REACTION

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References (6)

Publisher
American Medical Association
Copyright
Copyright © 1916 American Medical Association. All Rights Reserved. Applicable FARS/DFARS Restrictions Apply to Government Use.
ISSN
0096-8994
eISSN
1538-3628
DOI
10.1001/archpedi.1916.04110100026003
Publisher site
See Article on Publisher Site

Abstract

The practical value that the Schick reaction1 has acquired in the diagnosis of susceptibility or immunity to diphtheria makes it desirable to say a few words about the toxin used in the test, the overneutralized or the heated toxin used in the control test, about the technic of the test, and finally about the interpretation of the reactions. The diphtheria toxin for use in the Schick test consists of a broth culture of the diphtheria bacillus, which has been grown in the thermostat at 37 C. for six days. To kill the living organisms 10 parts of a 5 per cent. solution of phenol (carbolic acid) (0.5 per cent.) are then added, and the bacteria allowed to sediment by keeping the broth culture in the ice box during the following two or three days. The supernatant culture fluid is now passed through a Berkefeld filter, and the clear filtrate

Journal

American journal of diseases of children – American Medical Association

Published: Apr 1, 1916

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