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J. Woessner (1961)
The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid.Archives of biochemistry and biophysics, 93
R. Hubbell, G. Zalzal, R. Cotton, A. McAdams (1988)
Irradiated costal cartilage graft in experimental laryngotracheal reconstruction.International journal of pediatric otorhinolaryngology, 15 1
F. Luyten, G. Verbruggen, E. Veys, E. Goffin, H. Pypere (1987)
In vitro repair potential of articular cartilage: proteoglycan metabolism in the different areas of the femoral condyles in human cartilage explants.The Journal of rheumatology, 14 2
G. Verbruggen, F. Luyten, E. Veys (1985)
Repair function in organ cultured human cartilage. Replacement of enzymatically removed proteoglycans during longterm organ culture.The Journal of rheumatology, 12 4
W. Green (1971)
Behavior of articular chondrocytes in cell culture.Clinical orthopaedics and related research, 75
Cotton RT Zalzal GH (1986)
Cartilage graft: present statusHead Neck Surg, 8
G. Zalzal, R. Cotton, A. McAdams (1986)
The Survival of Costal Cartilage Graft in Laryngotracheal ReconstructionOtolaryngology-Head and Neck Surgery, 94
U. Vetter, J. Zapf, W. Heit, G. Helbing, E. Heinze, E. Froesch, W. Teller (1986)
Human fetal and adult chondrocytes. Effect of insulinlike growth factors I and II, insulin, and growth hormone on clonal growth.The Journal of clinical investigation, 77 6
Zapf J Vatter U (1986)
Human fetal and adult chondrocytes: effect of IGF-1 and IGF-2, insulin, and growth hormone on clonal growthJ Clin Invest, 77
M. Lammi, M. Tammi (1988)
Densitometric assay of nanogram quantities of proteoglycans precipitated on nitrocellulose membrane with Safranin O.Analytical biochemistry, 168 2
F. Luyten, V. Hascall, S. Nissley, T. Morales, A. Reddi (1988)
Insulin-like growth factors maintain steady-state metabolism of proteoglycans in bovine articular cartilage explants.Archives of biochemistry and biophysics, 267 2
C. Labarca, K. Paigen (1980)
A simple, rapid, and sensitive DNA assay procedure.Analytical biochemistry, 102 2
Veys EM Verbruggen G (1985)
Degenerative Joints
W. Daughaday, K. Hall, M. Raben, W. Salmon, J. Brande, J. Wyk (1972)
Somatomedin: Proposed Designation for Sulphation FactorNature, 235
R. Ham, G. Sattler (1968)
Clonal growth of differentiated rabbit cartilage cellsJournal of Cellular Physiology, 72
V. Hascall, C. Handley, D. McQuillan, G. Hascall, H. Robinson, D. Lowther (1983)
The effect of serum on biosynthesis of proteoglycans by bovine articular cartilage in culture.Archives of biochemistry and biophysics, 224 1
K. Kuettner, B. Pauli, G. Gall, V. Memoli, R. Schenk (1982)
Synthesis of cartilage matrix by mammalian chondrocytes in vitro. I. Isolation, culture characteristics, and morphologyThe Journal of Cell Biology, 93
G. Zalzal, C. Barber, R. Chandra (1989)
Tracheal Reconstruction Using Irradiated Homologous Grafts in RabbitsOtolaryngology–Head and Neck Surgery, 100
Pauli BU Kuettner KE (1982)
Synthesis of cartilage matrix by mammalian chondrocytes in vitro, I: isolation, culture characteristics, and morphologyCell Biol, 93
Memoli VA Kuettner KE (1982)
Synthesis of cartilage matrix by mammalian chondrocytes in vitro, II: maintenance of collagen and proteo glycan phenotypeCell Biol, 93
Abstract • Over the past several years it has become evident that expansion laryngotracheal surgery is effective in the treatment of laryngotracheal stenosis. Several clinical and animal studies have been performed to study the process of laryngotracheal stenosis and its treatment. However, there are still many questions that cannot be addressed by currently used clinical and animal research. Further indepth study of the behavior of the subglottis at the cellular level is necessary. We present an in vitro model for studying chondrocyte metabolism of the bovine cricoid. Cartilage was successfully grown in an explant culture system, and it was shown that the chondrocytes were metabolically active and responded to external agents. This model will serve to study the mechanism of growth and effects of trauma and external agents on the cricoid at the cellular level. (Arch Otolaryngol Head Neck Surg. 1992;118:407-411) References 1. Zalzal GH, Cotton RT, McAdams AJ. The survival of costal cartilage graft in laryngotracheal reconstruction . Otolaryngol Head Neck Surg . 1986;94:204-211. 2. Zalzal GH, Cotton RT, McAdams AJ. Cartilage graft: present status . Head Neck Surg . 1986;8:363-374.Crossref 3. Hubble RN, Zalzal GH, Cotton RT, McAdams AJ. Irradiated costal cartilage grafts in experimental laryngotracheal reconstruction . Int J Pediatr Otorhinolaryngol . 1988;15:67-72.Crossref 4. Zalzal GH, Barber CS, Chandra R. Tracheal reconstruction using irradiated homologous grafts in rabbits . Otolaryngol Head Neck Surg . 1989;100:119-125. 5. Daughaday WH, Hall K, Raben MS, Salmon WD, Van Den Brande JL, Van Wyk JJ. Somatomedin: proposed designation for sulfation factor . Nature . 1972;235:107.Crossref 6. Vatter U, Zapf J, Heit H, et al. Human fetal and adult chondrocytes: effect of IGF-1 and IGF-2, insulin, and growth hormone on clonal growth . J Clin Invest . 1986;77:1903-1908.Crossref 7. Luyten FP, Hascall VC, Nissley SP, Morales TI, Reddi AH. Insulinlike growth factors maintain steady state metabolism of proteoglycans in bovine articular cartilage explants . Arch Biochem Biophys . 1988;267:416-425.Crossref 8. Kuettner KE, Pauli BU, Gall G, Memoli VA, Schenk RK. Synthesis of cartilage matrix by mammalian chondrocytes in vitro, I: isolation, culture characteristics, and morphology . Cell Biol . 1982;93:743-750.Crossref 9. Kuettner KE, Memoli VA, Pauli BU, et al. Synthesis of cartilage matrix by mammalian chondrocytes in vitro, II: maintenance of collagen and proteo glycan phenotype . Cell Biol . 1982;93:751-757.Crossref 10. Verbruggen G, Veys EM, Luyten FB. Is a decrease in proteoglycan content in degenerative cartilage exclusively caused by enzymatic degradation? In: Verbruggen G, Veys EM, eds. Degenerative Joints . Princeton, NJ: Excerpta Medica; 1985:55-62. 11. Hascall VC, Handley CJ, McQuillan DJ, Hacall GK, Robinson HC, Lowther DA. The effect of serum on biosynthesis of proteoglycans by bovine articular cartilage in culture . Arch Biochem Biophys . 1983;224:206-223.Crossref 12. Verbruggen G, Luyten FP, Veys EM. Repair function and organ culture of the human cartilage: replacement of enzymatically removed proteoglycans during long term organ culture . J Rheumatol . 1985;12:665-674. 13. Luyten FP, Verbruggen G, Veys EM, De Pypere H. In vitro repair potential of articular cartilage: proteoglycan metabolism in the different areas of the femoral condyles in human cartilage explants . J Rheumatol . 1987;14:329-334. 14. Woessner JF Jr. The determination of hydroxyproline in tissue and protein samples containing small proportions of this amino acid . Arch Biochem Biophys . 1961;93:440-447.Crossref 15. Lammi M, Tammi M. Densitometric assay of nanogram quantities of proteoglycans precipitated on nitrocellulose membrane with safranin O . Anal Biochem . 1988;168:352-357.Crossref 16. Labarca C, Paigen K. A simple rapid and sensitive DNA assay procedure . Anal Biochem . 1980;102:344-352.Crossref 17. Ham RG, Sattler GL. Clonal growth of differentiated rabbit cartilage cells . J Cell Physiol . 1968;72:109-114.Crossref 18. Green WT. Behavior of articular chondrocytes in cell culture . Clin Orthop . 1971;75:248-260.Crossref
Archives of Otolaryngology - Head & Neck Surgery – American Medical Association
Published: Apr 1, 1992
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