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Inner Ear Damage Secondary to Diabetes Mellitus: II. Changes in Aging SHR/N-cp Rats

Inner Ear Damage Secondary to Diabetes Mellitus: II. Changes in Aging SHR/N-cp Rats Abstract • The congenic spontaneous hypertensive/National Institutes of Health (Bethesda, Md)–corpulent rat (SHR/N-cp) is a model for non–insulin-dependent diabetes mellitus. A previous study in our laboratory found significant loss of outer hair cells (OHC) in diabetic rats at 5.0 months of age. Our present study was designed to further evaluate the effects of the diabetic state on the inner ear in 10.5-month-old rats. The following comparisons were made: diabetic vs euglycemic control animals; obese vs lean phenotypes; and sucrose vs starch as the source of dietary carbohydrate. Cochleas were removed, fixed, stained, mounted on slides, and analyzed for OHC loss. We found a significant OHC loss in the cochleas of all diabetic animals. No statistical difference was found when comparing obese and lean phenotypes. Increased OHC loss was observed in all sucrose-fed vs starch-fed diabetic animals, although this increase was not statistically significant. Compared with an earlier study, an increase in OHC loss was also noted in the 10.5-month-old lean SHR/N-cp animals. Our results indicate that there is a relationship between non–insulin-dependent diabetes mellitus and inner ear damage and suggest that OHC loss is related to hyperglycemia and a genetic predisposition for glucose intolerance. (Arch Otolaryngol Head Neck Surg. 1992;118:397-400) References 1. Braunwald E, Isselbacher KJ, Petersdorf RG, et al. Harrison's Principles of Internal Medicine . 11th ed. New York, NY: McGraw-Hill International Book Co; 1987:1778-1796. 2. Wyngaarden JB, Smith LH, eds. Cecil's Textbook of Medicine . 18th ed. Philadelphia, Pa: WB Saunders Co; 1988:1361-1362. 3. Jordao AMD, cited in Jorgensen MB, Buch NH. Function of inner ear and cranial nerves in pregnant diabetics: clinical studies . Pract Otorhinolaryngol . 1962;24:111-116. 4. Harner SG. Hearing in adult-onset diabetes mellitus . Otolaryngol Head Neck Surg . 1981;89:322-327. 5. Axelsson A, Sigroth K, Vertes D. Hearing in diabetics . Acta Otolaryngol . 1978;356( (suppl) ):1-23. 6. Gibbon KP, Davis CG. A hearing survey in diabetes mellitus . Clin Otolaryngol . 1981;6:345-350.Crossref 7. Jorgensen MB. The inner ear in diabetes mellitus . Arch Otolaryngol . 1961;74:373-381.Crossref 8. Jannulis G, Delijannis G. Diabetes und Gicht als Ursache von kochlear Isschadigump . Monatsschr Ohrenheilkd . 1936;70:1504-1508. 9. Hansen CT. The development of the SHR/N- and LA/N-cp (corpulent) congenic rat strains . In; Hansen CT, d Michaelis IV OE, eds. New Model of Genetically Obese Rats for Studies in Diabetes, Heart Disease, and Complications of Obesity: Summaries of Workshop Papers and Current Bibliography . Bethesda, Md: National Institutes of Health;1988:7-10. 10. Michaelis OE, Carswell N, Hansen CT, Canary JJ, Kinnel PL. A new genetic model of non-insulin dependent diabetes and hypertension: the spontaneous hypertensive/NIH-corpulent rat . In: Shafrir E, Renold AE, eds. Frontiers in Diabetes Research: Lessons From Animal Diabetes . London, England: John Libbey; 1988;2:257-264. 11. Triana RJ, Suits GW, Garrison S, et al. Inner ear damage secondary to diabetes mellitus, I: changes in adolescent SHR/N-cp rats . Arch Otolaryngol Head Neck Surg . 1991;117:635-640.Crossref 12. Report of the American Institute of Nutrition Ad Hoc Committee on Standards for Nutritional Studies . J Nutr . 1977;107:1340-1348. 13. Michaelis OE IV, Ellwood KC, Hallfrisch J, et al. Effect of dietary sucrose and genotype on metabolic parameters of a new strain of genetically obese rat: LA/N-corpulent . Nutr Res . 1983;3:217-228.Crossref 14. Pillsbury HC. Hypertension, hyperlipoproteinemia, chronic noise exposure: is there synergism in cochlear pathology? Laryngoscope . 1986;96:1112-1138.Crossref 15. Steedman L. Alcian blue 8GS for mucins . Q J Microscop Sci . 1950;91:477. 16. Taylor IG, Irwin J. Some audiological aspects of diabetes mellitus . J Laryngol Otol . 1978;92:99-113.Crossref 17. Tooke JE. The microcirculation in diabetes . Diabetic Med . 1987;4:189-196.Crossref 18. Thalmann R, Marcus DC, Thalmann I. Biochemistry of the inner ear . Birth Defects . 1980;16:83-105. 19. Brownlee M, Cerami A, Vlassara H. Advanced glycosylation end products in tissue and the biochemical basis of diabetic complications . N Engl J Med . 1988;318:1315-1321.Crossref 20. Taylor R, Agius L. The biochemistry of diabetes . Biochem J . 1988;250:625-640. 21. Burg MB, Kador PF. Sorbitol, osmoregulation, and the complications of diabetes . J Clin Invest . 1988;81:635-640.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Otolaryngology - Head & Neck Surgery American Medical Association

Inner Ear Damage Secondary to Diabetes Mellitus: II. Changes in Aging SHR/N-cp Rats

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References (22)

Publisher
American Medical Association
Copyright
Copyright © 1992 American Medical Association. All Rights Reserved.
ISSN
0886-4470
eISSN
1538-361X
DOI
10.1001/archotol.1992.01880040059010
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Abstract

Abstract • The congenic spontaneous hypertensive/National Institutes of Health (Bethesda, Md)–corpulent rat (SHR/N-cp) is a model for non–insulin-dependent diabetes mellitus. A previous study in our laboratory found significant loss of outer hair cells (OHC) in diabetic rats at 5.0 months of age. Our present study was designed to further evaluate the effects of the diabetic state on the inner ear in 10.5-month-old rats. The following comparisons were made: diabetic vs euglycemic control animals; obese vs lean phenotypes; and sucrose vs starch as the source of dietary carbohydrate. Cochleas were removed, fixed, stained, mounted on slides, and analyzed for OHC loss. We found a significant OHC loss in the cochleas of all diabetic animals. No statistical difference was found when comparing obese and lean phenotypes. Increased OHC loss was observed in all sucrose-fed vs starch-fed diabetic animals, although this increase was not statistically significant. Compared with an earlier study, an increase in OHC loss was also noted in the 10.5-month-old lean SHR/N-cp animals. Our results indicate that there is a relationship between non–insulin-dependent diabetes mellitus and inner ear damage and suggest that OHC loss is related to hyperglycemia and a genetic predisposition for glucose intolerance. (Arch Otolaryngol Head Neck Surg. 1992;118:397-400) References 1. Braunwald E, Isselbacher KJ, Petersdorf RG, et al. Harrison's Principles of Internal Medicine . 11th ed. New York, NY: McGraw-Hill International Book Co; 1987:1778-1796. 2. Wyngaarden JB, Smith LH, eds. Cecil's Textbook of Medicine . 18th ed. Philadelphia, Pa: WB Saunders Co; 1988:1361-1362. 3. Jordao AMD, cited in Jorgensen MB, Buch NH. Function of inner ear and cranial nerves in pregnant diabetics: clinical studies . Pract Otorhinolaryngol . 1962;24:111-116. 4. Harner SG. Hearing in adult-onset diabetes mellitus . Otolaryngol Head Neck Surg . 1981;89:322-327. 5. Axelsson A, Sigroth K, Vertes D. Hearing in diabetics . Acta Otolaryngol . 1978;356( (suppl) ):1-23. 6. Gibbon KP, Davis CG. A hearing survey in diabetes mellitus . Clin Otolaryngol . 1981;6:345-350.Crossref 7. Jorgensen MB. The inner ear in diabetes mellitus . Arch Otolaryngol . 1961;74:373-381.Crossref 8. Jannulis G, Delijannis G. Diabetes und Gicht als Ursache von kochlear Isschadigump . Monatsschr Ohrenheilkd . 1936;70:1504-1508. 9. Hansen CT. The development of the SHR/N- and LA/N-cp (corpulent) congenic rat strains . In; Hansen CT, d Michaelis IV OE, eds. New Model of Genetically Obese Rats for Studies in Diabetes, Heart Disease, and Complications of Obesity: Summaries of Workshop Papers and Current Bibliography . Bethesda, Md: National Institutes of Health;1988:7-10. 10. Michaelis OE, Carswell N, Hansen CT, Canary JJ, Kinnel PL. A new genetic model of non-insulin dependent diabetes and hypertension: the spontaneous hypertensive/NIH-corpulent rat . In: Shafrir E, Renold AE, eds. Frontiers in Diabetes Research: Lessons From Animal Diabetes . London, England: John Libbey; 1988;2:257-264. 11. Triana RJ, Suits GW, Garrison S, et al. Inner ear damage secondary to diabetes mellitus, I: changes in adolescent SHR/N-cp rats . Arch Otolaryngol Head Neck Surg . 1991;117:635-640.Crossref 12. Report of the American Institute of Nutrition Ad Hoc Committee on Standards for Nutritional Studies . J Nutr . 1977;107:1340-1348. 13. Michaelis OE IV, Ellwood KC, Hallfrisch J, et al. Effect of dietary sucrose and genotype on metabolic parameters of a new strain of genetically obese rat: LA/N-corpulent . Nutr Res . 1983;3:217-228.Crossref 14. Pillsbury HC. Hypertension, hyperlipoproteinemia, chronic noise exposure: is there synergism in cochlear pathology? Laryngoscope . 1986;96:1112-1138.Crossref 15. Steedman L. Alcian blue 8GS for mucins . Q J Microscop Sci . 1950;91:477. 16. Taylor IG, Irwin J. Some audiological aspects of diabetes mellitus . J Laryngol Otol . 1978;92:99-113.Crossref 17. Tooke JE. The microcirculation in diabetes . Diabetic Med . 1987;4:189-196.Crossref 18. Thalmann R, Marcus DC, Thalmann I. Biochemistry of the inner ear . Birth Defects . 1980;16:83-105. 19. Brownlee M, Cerami A, Vlassara H. Advanced glycosylation end products in tissue and the biochemical basis of diabetic complications . N Engl J Med . 1988;318:1315-1321.Crossref 20. Taylor R, Agius L. The biochemistry of diabetes . Biochem J . 1988;250:625-640. 21. Burg MB, Kador PF. Sorbitol, osmoregulation, and the complications of diabetes . J Clin Invest . 1988;81:635-640.Crossref

Journal

Archives of Otolaryngology - Head & Neck Surgery – American Medical Association

Published: Apr 1, 1992

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