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Immunomodulation of Nodal Lymphocytes in Head and Neck Cancer

Immunomodulation of Nodal Lymphocytes in Head and Neck Cancer Abstract • The purpose of this study was to evaluate whether the interaction of human neck node lymphocytes with primary tumor cells could be modulated with the administration of bacillus Calmette-Guérin (BCG) or N-acetylmuramyldipeptide (MDP). The procedures involve the clonogenic assay applied towards studying the interactions of neck node lymphocytes with the tumor-stem cell population. It has been previously demonstrated that a dynamic interaction occurs between neck nodal lymphocytes and tumor. Bacillus Calmette-Guérin or MDP was incubated for three days with lymphocytes from neck nodes, after which the human squamous tumor cells from the patient were exposed continuously to the resulting lymphokines in soft agar. Ninety percent of the cases studied exhibit inhibition of tumor cell proliferation with BCG-or MDP-treated lymphocytes. The data suggest both BCG and MDP may enhance regional nodal lymphocyte tumor inhibition. (Arch Otolaryngol 1985;111:465-468) References 1. Schuller DE, McGuirt WF, McCabe BF, et al: The prognostic significance of metastatic lymph nodes . Laryngoscope 1980;40:557-570. 2. Hamburger AW, Salmon SE: Development of a bioassay for human myeloma colony-forming cells , in Cloning of Human Tumor Stem Cells . New York, Alan R Liss, 1980, vol 48, pp 23-41. 3. Hamburger AW, Salmon SE: Primary bioassay of human tumor stem cells . Science 1977; 197:461-463.Crossref 4. VanHoff DO, Casper J, Bradley E, et al: Direct cloning of human neuroblastoma cells on soft agar culture . Cancer Res 1980;40:3591-3597. 5. Hamburger AW, Salmon SE: Primary bioassay of human myeloma stem cells . J Clin Invest 1977;60:846-854.Crossref 6. Zwilling BS, Campolito LB: Destruction of tumor cells by BCG-activated alveolar macrophages . J Immunol 1977;119:838. 7. Olivotto M, Bomford R: In vitro inhibition of tumor cell growth and DNA synthesis by peritoneal and lung macrophages from mice injected with Corynebacterium parvum . Int J Cancer 1974;13:478.Crossref 8. Sone S, Fidler J: In situ activation of tumoricidal properties in rat alveolar macrophages and rejection of experimental lung metastases by intravenous injection of Nocardia rubra cell wall skeleton . Cancer Immunol Immunother 1982; 12:203Crossref 9. Ellouz F, Adams A, Ciobaru R, et al: Minimal structural requirements for adjuvant activity of bacterial peptidoglycan derivatives . Biochem Biophys Res Commun 1974;59:1317.Crossref 10. Kotani S, Watanabe Y, Kinoshita T, et al: Immunoadjuvant activities of synthetic N-acetylmuramyl peptides or amino acids . Biken J 1975;18:105. 11. Leclerc C, Bourgeous E: Demonstration of muramyl dipeptide (MDP): Induced T suppressor cells responsible for MDP immunosuppressive activity . Eur J Immunol 1982;12:249-254.Crossref 12. Fidler IJ, Poste G: Activation of tumoricidal properties in murine macrophages by intravenous injection of muramyl dipeptide encapsulated within liposomes as a treatment for spontaneous metastasis , in The Prostatic Cell: Structure and Function . New York, Alan R Liss Inc, 1981, pp 257-267. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Otolaryngology American Medical Association

Immunomodulation of Nodal Lymphocytes in Head and Neck Cancer

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References (14)

Publisher
American Medical Association
Copyright
Copyright © 1985 American Medical Association. All Rights Reserved.
ISSN
0003-9977
DOI
10.1001/archotol.1985.00800090079013
Publisher site
See Article on Publisher Site

Abstract

Abstract • The purpose of this study was to evaluate whether the interaction of human neck node lymphocytes with primary tumor cells could be modulated with the administration of bacillus Calmette-Guérin (BCG) or N-acetylmuramyldipeptide (MDP). The procedures involve the clonogenic assay applied towards studying the interactions of neck node lymphocytes with the tumor-stem cell population. It has been previously demonstrated that a dynamic interaction occurs between neck nodal lymphocytes and tumor. Bacillus Calmette-Guérin or MDP was incubated for three days with lymphocytes from neck nodes, after which the human squamous tumor cells from the patient were exposed continuously to the resulting lymphokines in soft agar. Ninety percent of the cases studied exhibit inhibition of tumor cell proliferation with BCG-or MDP-treated lymphocytes. The data suggest both BCG and MDP may enhance regional nodal lymphocyte tumor inhibition. (Arch Otolaryngol 1985;111:465-468) References 1. Schuller DE, McGuirt WF, McCabe BF, et al: The prognostic significance of metastatic lymph nodes . Laryngoscope 1980;40:557-570. 2. Hamburger AW, Salmon SE: Development of a bioassay for human myeloma colony-forming cells , in Cloning of Human Tumor Stem Cells . New York, Alan R Liss, 1980, vol 48, pp 23-41. 3. Hamburger AW, Salmon SE: Primary bioassay of human tumor stem cells . Science 1977; 197:461-463.Crossref 4. VanHoff DO, Casper J, Bradley E, et al: Direct cloning of human neuroblastoma cells on soft agar culture . Cancer Res 1980;40:3591-3597. 5. Hamburger AW, Salmon SE: Primary bioassay of human myeloma stem cells . J Clin Invest 1977;60:846-854.Crossref 6. Zwilling BS, Campolito LB: Destruction of tumor cells by BCG-activated alveolar macrophages . J Immunol 1977;119:838. 7. Olivotto M, Bomford R: In vitro inhibition of tumor cell growth and DNA synthesis by peritoneal and lung macrophages from mice injected with Corynebacterium parvum . Int J Cancer 1974;13:478.Crossref 8. Sone S, Fidler J: In situ activation of tumoricidal properties in rat alveolar macrophages and rejection of experimental lung metastases by intravenous injection of Nocardia rubra cell wall skeleton . Cancer Immunol Immunother 1982; 12:203Crossref 9. Ellouz F, Adams A, Ciobaru R, et al: Minimal structural requirements for adjuvant activity of bacterial peptidoglycan derivatives . Biochem Biophys Res Commun 1974;59:1317.Crossref 10. Kotani S, Watanabe Y, Kinoshita T, et al: Immunoadjuvant activities of synthetic N-acetylmuramyl peptides or amino acids . Biken J 1975;18:105. 11. Leclerc C, Bourgeous E: Demonstration of muramyl dipeptide (MDP): Induced T suppressor cells responsible for MDP immunosuppressive activity . Eur J Immunol 1982;12:249-254.Crossref 12. Fidler IJ, Poste G: Activation of tumoricidal properties in murine macrophages by intravenous injection of muramyl dipeptide encapsulated within liposomes as a treatment for spontaneous metastasis , in The Prostatic Cell: Structure and Function . New York, Alan R Liss Inc, 1981, pp 257-267.

Journal

Archives of Otolaryngology – American Medical Association

Published: Jul 1, 1985

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