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Intracellular Potential Changes of Corti's Organ With Anoxia

Intracellular Potential Changes of Corti's Organ With Anoxia Abstract • Intracellular measurements of the resting cell membrane potentials of guinea pig Corti's organ were made in order to determine the sensitivity of this cell potential to anoxic hypoxia (a lowered oxygen state due to lack of respiratory oxygen) and to establish differences according to cell types or morphologic regions of the sensory epithelium. The negative cell potentials measured from successful electrode penetrations were found to be relatively more stable and resistant to change during a 120-s period of anoxia than was the positive endocochlear potential. The intracellular resting potentials were also much slower to recover after resumption of respiration. Data obtained from various cells in two different regions of Corti's organ indicate that Corti's organ is receiving oxygen from both the perilymph and the endolymph. An iontophoretic dye-marking technique was used to label some experimental cells for later histologic identification. (Arch Otolaryngol 105:574-578, 1979) References 1. Lawrence M, Nuttall AL: Oxygen availability in tunnel of Corti of its extracellular fluids . Laryngoscope 84:1767-1776, 1974.Crossref 2. Lawrence M, Nuttall AL, Clapper MP: Electrical potentials and fluid boundaries within the organ of Corti . J Acoust Soc Am 55:122-138, 1974.Crossref 3. Konishi T, Butler RA, Fernandez C: Effect of anoxia on cochlear potentials . J Acoust Soc Am 33:349-356, 1961.Crossref 4. Davis H: A model for transducer action in the cochlea . Cold Spring Harbor Symp Quant Biol 30:181-190, 1965.Crossref 5. Lawrence M: Direct visualization of living organ of Corti and studies measured by microelectrode . J Acoust Soc Am 52:566-573, 1972.Crossref 6. Goto T: Experimental studies on the negative intracellular potential of the organ of Corti under various conditions . Transl Beltone Inst Hearing Res , vol 29, (February) 1975. 7. Konishi T, Yasuno T: Summating potentials of the cochlea in the guinea pig . J Acoust Soc Am 35:1448-1452, 1963.Crossref 8. Dallos P: On the negative potential within the organ of Corti . J Acoust Soc Am 44:818-819, 1968.Crossref 9. Peake WT, Sohmer H, Weiss TF: Microelectrode recordings of intracochlear potentials . Q Prog Rep Mass Inst Technol Res Lab Electronics 94:293-304, 1969. 10. Sohmer HS, Peake WT, Weiss TF: Intracochlear potential recorded with micropipette: I. Correlations with micropipette location . J Acoust Soc Am 50:572-580, 1971.Crossref 11. Russell I, Sellick P: Tuning properties of cochlear hair cells . Nature 267:858-860, 1977.Crossref 12. Lawrence M, Nuttall AL, Burgio PA: Cochlear potentials and oxygen associated with hypoxia . Ann Otol Rhinol Laryngol 84:499-512, 1975. 13. Thalmann R, Thalmann I, Comegys TH: Quantitative cytochemistry of the organ of Corti: Dissection, weight determination and analysis of single outer hair cells . Laryngoscope 82:2059-2078, 1972.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Otolaryngology American Medical Association

Intracellular Potential Changes of Corti's Organ With Anoxia

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References (16)

Publisher
American Medical Association
Copyright
Copyright © 1979 American Medical Association. All Rights Reserved.
ISSN
0003-9977
DOI
10.1001/archotol.1979.00790220008002
Publisher site
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Abstract

Abstract • Intracellular measurements of the resting cell membrane potentials of guinea pig Corti's organ were made in order to determine the sensitivity of this cell potential to anoxic hypoxia (a lowered oxygen state due to lack of respiratory oxygen) and to establish differences according to cell types or morphologic regions of the sensory epithelium. The negative cell potentials measured from successful electrode penetrations were found to be relatively more stable and resistant to change during a 120-s period of anoxia than was the positive endocochlear potential. The intracellular resting potentials were also much slower to recover after resumption of respiration. Data obtained from various cells in two different regions of Corti's organ indicate that Corti's organ is receiving oxygen from both the perilymph and the endolymph. An iontophoretic dye-marking technique was used to label some experimental cells for later histologic identification. (Arch Otolaryngol 105:574-578, 1979) References 1. Lawrence M, Nuttall AL: Oxygen availability in tunnel of Corti of its extracellular fluids . Laryngoscope 84:1767-1776, 1974.Crossref 2. Lawrence M, Nuttall AL, Clapper MP: Electrical potentials and fluid boundaries within the organ of Corti . J Acoust Soc Am 55:122-138, 1974.Crossref 3. Konishi T, Butler RA, Fernandez C: Effect of anoxia on cochlear potentials . J Acoust Soc Am 33:349-356, 1961.Crossref 4. Davis H: A model for transducer action in the cochlea . Cold Spring Harbor Symp Quant Biol 30:181-190, 1965.Crossref 5. Lawrence M: Direct visualization of living organ of Corti and studies measured by microelectrode . J Acoust Soc Am 52:566-573, 1972.Crossref 6. Goto T: Experimental studies on the negative intracellular potential of the organ of Corti under various conditions . Transl Beltone Inst Hearing Res , vol 29, (February) 1975. 7. Konishi T, Yasuno T: Summating potentials of the cochlea in the guinea pig . J Acoust Soc Am 35:1448-1452, 1963.Crossref 8. Dallos P: On the negative potential within the organ of Corti . J Acoust Soc Am 44:818-819, 1968.Crossref 9. Peake WT, Sohmer H, Weiss TF: Microelectrode recordings of intracochlear potentials . Q Prog Rep Mass Inst Technol Res Lab Electronics 94:293-304, 1969. 10. Sohmer HS, Peake WT, Weiss TF: Intracochlear potential recorded with micropipette: I. Correlations with micropipette location . J Acoust Soc Am 50:572-580, 1971.Crossref 11. Russell I, Sellick P: Tuning properties of cochlear hair cells . Nature 267:858-860, 1977.Crossref 12. Lawrence M, Nuttall AL, Burgio PA: Cochlear potentials and oxygen associated with hypoxia . Ann Otol Rhinol Laryngol 84:499-512, 1975. 13. Thalmann R, Thalmann I, Comegys TH: Quantitative cytochemistry of the organ of Corti: Dissection, weight determination and analysis of single outer hair cells . Laryngoscope 82:2059-2078, 1972.Crossref

Journal

Archives of Otolaryngology – American Medical Association

Published: Oct 1, 1979

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