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P032 Differential Protein Expression Patterns of p53, NF-κB, and NF-κB– Regulated Genes in Head and Neck Squamous Cell Carcinoma

P032 Differential Protein Expression Patterns of p53, NF-κB, and NF-κB– Regulated Genes in Head... Objective: The current study examines the protein expression and activation of NF-κB (p65) and its regulated genes in head and neck squamous cell carcinoma (HNSCC) specimens based on p53 status. Design: Head and neck squamous cell carcinoma tumor specimens were preserved in OCT and processed for frozen sections. Immunohistochemical staining of primary antibodies anti-p53 (DO1), phosphorylated NF-κB (p65), CA-9, YAP, c-IAP-1, ICAM-1, and HPV16-E6/18-E6 was carried out using the ABC-based method. Subjects: Twenty-five tumor specimens were obtained from the tongue, larynx, pharynx, oral cavity, and tonsil of HNSCC patients without prior treatment, through Cooperative Human Tissue Network (CHTN), Midwestern Division, Columbus, Ohio, and National Institutes of Health clinical protocols 04C0104 and 04C0141. Results: Head and neck squamous cell carcinoma with wild-type p53 (wt-p53) were found to display a reciprocal pattern of high phosphorylated NF-κB (p65) and low-negative p53 staining, in approximately 45% of all tumor samples examined. All tumors yielded staining of phospho-NF-κB (p65) and its downstream proteins (CA-9, YAP-65, c-IAP-1, and ICAM-1). These downstream proteins coincided with p65 staining location, while varying in staining intensity, heterogeneity, and patterns. Tumors with wt-p53 status revealed a significantly stronger protein expression than mt-p53 tumors for phospho-NF-κB (p65) and its downstream proteins. Conclusion: The reciprocal relationship of immunostaining in phosphorylated NF-κB (p65) and p53 indicated the importance of the activation of NF-κB and its downstream genes in the malignant progression of HNSCC, especially in tumors with wt-p53. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Otolaryngology - Head & Neck Surgery American Medical Association

P032 Differential Protein Expression Patterns of p53, NF-κB, and NF-κB– Regulated Genes in Head and Neck Squamous Cell Carcinoma

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P032 Differential Protein Expression Patterns of p53, NF-κB, and NF-κB– Regulated Genes in Head and Neck Squamous Cell Carcinoma

Abstract

Objective: The current study examines the protein expression and activation of NF-κB (p65) and its regulated genes in head and neck squamous cell carcinoma (HNSCC) specimens based on p53 status. Design: Head and neck squamous cell carcinoma tumor specimens were preserved in OCT and processed for frozen sections. Immunohistochemical staining of primary antibodies anti-p53 (DO1), phosphorylated NF-κB (p65), CA-9, YAP, c-IAP-1, ICAM-1, and HPV16-E6/18-E6 was carried out using the...
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Publisher
American Medical Association
Copyright
Copyright © 2006 American Medical Association. All Rights Reserved.
ISSN
0886-4470
eISSN
1538-361X
DOI
10.1001/archotol.132.8.871-b
Publisher site
See Article on Publisher Site

Abstract

Objective: The current study examines the protein expression and activation of NF-κB (p65) and its regulated genes in head and neck squamous cell carcinoma (HNSCC) specimens based on p53 status. Design: Head and neck squamous cell carcinoma tumor specimens were preserved in OCT and processed for frozen sections. Immunohistochemical staining of primary antibodies anti-p53 (DO1), phosphorylated NF-κB (p65), CA-9, YAP, c-IAP-1, ICAM-1, and HPV16-E6/18-E6 was carried out using the ABC-based method. Subjects: Twenty-five tumor specimens were obtained from the tongue, larynx, pharynx, oral cavity, and tonsil of HNSCC patients without prior treatment, through Cooperative Human Tissue Network (CHTN), Midwestern Division, Columbus, Ohio, and National Institutes of Health clinical protocols 04C0104 and 04C0141. Results: Head and neck squamous cell carcinoma with wild-type p53 (wt-p53) were found to display a reciprocal pattern of high phosphorylated NF-κB (p65) and low-negative p53 staining, in approximately 45% of all tumor samples examined. All tumors yielded staining of phospho-NF-κB (p65) and its downstream proteins (CA-9, YAP-65, c-IAP-1, and ICAM-1). These downstream proteins coincided with p65 staining location, while varying in staining intensity, heterogeneity, and patterns. Tumors with wt-p53 status revealed a significantly stronger protein expression than mt-p53 tumors for phospho-NF-κB (p65) and its downstream proteins. Conclusion: The reciprocal relationship of immunostaining in phosphorylated NF-κB (p65) and p53 indicated the importance of the activation of NF-κB and its downstream genes in the malignant progression of HNSCC, especially in tumors with wt-p53.

Journal

Archives of Otolaryngology - Head & Neck Surgery – American Medical Association

Published: Aug 1, 2006

Keywords: genes,head and neck squamous cell carcinoma

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