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ORIGINAL ARTICLE ONLINE FIRST Disinfection of Flexible Fiberoptic Laryngoscopes After In Vitro Contamination With Staphylococcus aureus and Candida albicans Dennis Chang, MD; Andrew Florea, MD; Mark Rowe, MD; Kristin A. Seiberling, MD Objective: To determine the efficacy of various clean- alcohol; a 30-second wipe with antibacterial soap, fol- ing and disinfective methods in reducing bacterial and lowed by a 30-second scrub with isopropyl alcohol; and a fungal load on flexible fiberoptic laryngoscopes (FFLs). 30-second wipe with germicidal cloth, all accompanied by previous rinsing with 30 seconds of running tap water. Design: In vitro model. Results: All protocols except the isolated 5-minute soak Subjects: Flexible fiberoptic laryngoscopes contami- in enzymatic soap solution were successful in com- nated with Staphylococcus aureus and Candida albicans. pletely disinfecting the FFLs after experimental contami- nation with S aureus or C albicans. Interventions: Contamination with S aureus and C al- bicans was separately induced on FFLs, which were then Conclusion: Various different cleaning methods ap- disinfected with different protocols: 20-, 15-, 10-, and 5-min- peared to properly disinfect FFLs after inoculation with ute soaks in ortho-phthalaldehyde (Cidex OPA; Johnson S aureus and C albicans in an in vitro model. & Johnson) with or without presoaking
JAMA Otolaryngology - Head & Neck Surgery – American Medical Association
Published: Feb 1, 2012
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