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- Publisher
- American Medical Association
- Copyright
- Copyright © 1987 American Medical Association. All Rights Reserved.
- ISSN
- 0003-9950
- eISSN
- 1538-3687
- DOI
- 10.1001/archopht.1987.01060020114041
- Publisher site
- See Article on Publisher Site
Abstract
Abstract • We determined the immunoreactive opsin content in the subretinal fluid from 16 patients with rhegmatogenous retinal detachment by enzyme-linked immunosorbent assay. The opsin content decreased with the duration of the detachment, but there was no correlation between opsin content and the age of the patient or the extent of the retinal detachment. References 1. Feeney L, Burns RP, Mixon RM: Human subretinal fluid: Its cellular and subcellular components . Arch Ophthalmol 1975;93:62-69.Crossref 2. Kroll AL, Machemer R: Experimental retinal detachment in the owl monkey: III. Electron microscopy of retina and pigment epithelium . Am J Ophthalmol 1968;66:410-427. 3. Plantner JJ, Kean EL: Carbohydrate composition of bovine rhodopsin . J Biol Chem 1976;251:1548-1552. 4. Laemmli UK: Cleavage of structural proteins during assembly of the head of bacteriophage T4 . Nature 1970;227:680-685.Crossref 5. Towbin H, Staehelim T, Gordon J: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications . Proc Natl Acad Sci USA 1979;76:4350-4354.Crossref 6. Hubbard R, Brown PK, Bownds D: Methodology of vitamin A and visual pigment: III. Rod opsin , in McCormic DB, Wright LD (eds): Method in Enzymology . Orlando, Fla, Academic Press Inc, 1971, vol 18, pt C, pp 623-625. 7. Paanakker JE, Groenedijk GWT: Separation of geometric isomers of retinyl ester, retinal and retinol, pertaining to the visual cycle, by high-performance liquid chromatography . J Chromatogr 1979;168:125-132.Crossref 8. Wald G, Brown PK: Human rhodopsin . Science 1958;127:222-226.Crossref 9. Gregerson DS, Abrahams IW, Thirkill CE: Serum antibody levels of uveitis patients to bovine retinal antigens . Invest Ophthalmol Vis Sci 1981;21:669-680. 10. Hayasaka S, Hara S, Mizuno K: Lysosomal enzymes in subretinal fluid . Graefes Arch Clin Exp Ophthalmol 1976;200:13-20.Crossref 11. Hayasaka S, Hara S, Mizuno K: Degradation of rod outer segment proteins by cathepsin D . J Biochem 1975;78:1365-1367. 12. Hara S, Plantner JJ, Kean EL: The enzymatic cleavage of rhodopsin by the retinal pigment epithelium: I. Enzyme preparation, properties and kinetics: Characterization of the glycopeptide product . Exp Eye Res 1983;36:799-816.Crossref 13. Dowling JE, Gibbon IR: The fine structure of the pigment epithelium in the albino rat . J Cell Biol 1962;14:459-474.Crossref 14. Kranias G, Kranias E, Dobbie JG: Protein kinase in the subretinal fluid . Exp Eye Res 1979;29:1-6.Crossref 15. Plantner JJ, Hara S, Kean EL: Improved, rapid radioimmunoassay for rhodopsin . Exp Eye Res 1982;35:543-548.Crossref 16. Crescitelli F, Dartnall HJA: Human visual purple . Nature 1953;172:195-197.Crossref 17. Barbour HL, Plantner JJ, Kean EL, et al: Rhodopsin content of human retinas . Invest Ophthalmol Vis Sci 1985;26( (suppl) ):290.
Journal
Archives of Ophthalmology – American Medical Association
Published: Feb 1, 1987