Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

A Medium-Term Corneal Preserving Medium (K-Sol)

A Medium-Term Corneal Preserving Medium (K-Sol) Abstract • A new corneal preserving solution, K-Sol, was prepared by combining TC 199, 100 mg/L of gentamicin sulfate, 0.025M HEPES buffer to a pH of 7.4, and various concentrations (2.0%; 2.25%; 2.5%) of chondroitin sulfate to a final osmolality of 310 mOsm. Rabbit corneas stored at 4° C for 12 days in each solution, as well as fresh controls, were used for penetrating keratoplasty. Three weeks later, one graft in the 2.0% group was opacified; the rest were clear and showed minimal thickening. There were no significant differences between controls in terms of pachymetry and endothelial cell loss. This study supports the finding that K-Sol with 2.5% chondroitin sulfate is a simple, effective, mediumterm preserving solution for donor tissue for penetrating keratoplasty in rabbits. References 1. McCarey BE, Kaufman HE: Improved corneal storage . Invest Ophthalmol Vis Sci 1974; 13:165-173. 2. McCarey BE, Meyer RF, Kaufman HE: Improved corneal storage for penetrating keratoplasties in humans . Ann Ophthalmol 1976;8:1488-1495. 3. Capella JA: Techniques of corneal cryopreservation , in Capella JA, Edelhauser HF, Van Horn DL (eds): Corneal Preservation . Springfield, Ill, Charles C Thomas Publisher, 1973, pp 308-317. 4. Kaufman HE: Corneal cryopreservation and its clinical application . Transplant Proc 1976;8( (suppl 1) ):149-152. 5. Doughman DJ, Harris JE, Mindrup E, et al: Prolonged donor corneal preservation in organ culture: Long-term clinical evaluation . Cornea 1982;1:7-20. 6. Kaufman HE, Varnell ED, Kaufman S: Chondroitin sulfate in a new corneal preservation medium . Am J Ophthalmol 1984;98:112-114.Crossref 7. Kaufman HE, Varnell ED, Kaufman S, et al: K-Sol corneal preservation . Am J Ophthalmol 1985;100:299-304. 8. Blackwell WL, Gravenstein N, Kaufman HE: Comparison of central corneal endothelial cell numbers with peripheral areas . Am J Ophthalmol 1977;84:473-476. 9. Van Horn DL, Sendele DD, Seideman S, et al: Regenerative capacity of the corneal endothelium in rabbit and cat . Invest Ophthalmol Vis Sci 1977;16:597-613. 10. Condon PI, Fitzgerald G, Burke A, et al: The physical effects of viscoelastic substances on human donor cornea . Trans Ophthalmol Soc UK 1983;103:265-267. 11. Harrison SE, Soll DB, Shayegan M, et al: Chondroitin sulfate: A new and effective protective agent for intraocular lens insertion . Ophthalmology 1982;89:1254-1260.Crossref 12. MacRae SM, Edelhauser HF, Hyndiuk RA, et al: The effects of sodium hyaluronate, chondroitin sulfate, and methylcellulose on the corneal endothelium and intraocular pressure . Am J Ophthalmol 1983;95:332-341. 13. Carty JB: Chondroitin sulfate in anterior segment surgery . Trans Ophthalmol Soc UK 1983; 103:263-264. 14. Ng MC, Riley MV: Relation of intracellular levels and redox state of glutathione to endothelial function in the rabbit cornea . Exp Eye Res 1980;30:511-517.Crossref 15. Edelhauser HF, Van Horn DL, Miller P, et al: Effect of thiol-oxidation of glutathione with diamide on corneal endothelial function, junctional complexes, and microfilaments . J Cell Biol 1976;68:567-578.Crossref 16. Hull DS, Green K, Bowman K, et al: Intracellular pH and glutathione levels in rabbit corneal endothelium following storage in moist chamber and MK medium . Invest Ophthalmol Vis Sci 1983;24:214-217. 17. Trawkin AG, Gundorowa RA, Bordjugowa GG, et al: Erforschung des Zustandes der Zellmembran und der Mechanismen der Hornhautautolyse bei der Konservierung . Klin Monatsbl Augenheilkd 1976;169:500-504. 18. Hull DS, Strickland EC, Green K: Photodynamically induced alteration of cornea endothelial cell function . Invest Ophthalmol Vis Sci 1979;18:1226-1231. 19. Edelhauser HF, Hanneken AM, Pederson HJ, et al: Osmotic tolerance of rabbit and human corneal endothelium . Arch Ophthalmol 1981; 99:1281-1287.Crossref 20. Gonnering R, Edelhauser HF, Van Horn DL, et al: The pH tolerance of rabbit and human corneal endothelium . Invest Ophthalmol Vis Sci 1979;18:373-390. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

A Medium-Term Corneal Preserving Medium (K-Sol)

Yau, Chi-Wang; Kaufman, Herbert E.
Archives of Ophthalmology , Volume 104 (4) – Apr 1, 1986
Download PDF
4 pages

Loading next page...
 
/lp/american-medical-association/a-medium-term-corneal-preserving-medium-k-sol-6eiWYp18mr

References (23)

Publisher
American Medical Association
Copyright
Copyright © 1986 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1986.01050160154032
Publisher site
See Article on Publisher Site

Abstract

Abstract • A new corneal preserving solution, K-Sol, was prepared by combining TC 199, 100 mg/L of gentamicin sulfate, 0.025M HEPES buffer to a pH of 7.4, and various concentrations (2.0%; 2.25%; 2.5%) of chondroitin sulfate to a final osmolality of 310 mOsm. Rabbit corneas stored at 4° C for 12 days in each solution, as well as fresh controls, were used for penetrating keratoplasty. Three weeks later, one graft in the 2.0% group was opacified; the rest were clear and showed minimal thickening. There were no significant differences between controls in terms of pachymetry and endothelial cell loss. This study supports the finding that K-Sol with 2.5% chondroitin sulfate is a simple, effective, mediumterm preserving solution for donor tissue for penetrating keratoplasty in rabbits. References 1. McCarey BE, Kaufman HE: Improved corneal storage . Invest Ophthalmol Vis Sci 1974; 13:165-173. 2. McCarey BE, Meyer RF, Kaufman HE: Improved corneal storage for penetrating keratoplasties in humans . Ann Ophthalmol 1976;8:1488-1495. 3. Capella JA: Techniques of corneal cryopreservation , in Capella JA, Edelhauser HF, Van Horn DL (eds): Corneal Preservation . Springfield, Ill, Charles C Thomas Publisher, 1973, pp 308-317. 4. Kaufman HE: Corneal cryopreservation and its clinical application . Transplant Proc 1976;8( (suppl 1) ):149-152. 5. Doughman DJ, Harris JE, Mindrup E, et al: Prolonged donor corneal preservation in organ culture: Long-term clinical evaluation . Cornea 1982;1:7-20. 6. Kaufman HE, Varnell ED, Kaufman S: Chondroitin sulfate in a new corneal preservation medium . Am J Ophthalmol 1984;98:112-114.Crossref 7. Kaufman HE, Varnell ED, Kaufman S, et al: K-Sol corneal preservation . Am J Ophthalmol 1985;100:299-304. 8. Blackwell WL, Gravenstein N, Kaufman HE: Comparison of central corneal endothelial cell numbers with peripheral areas . Am J Ophthalmol 1977;84:473-476. 9. Van Horn DL, Sendele DD, Seideman S, et al: Regenerative capacity of the corneal endothelium in rabbit and cat . Invest Ophthalmol Vis Sci 1977;16:597-613. 10. Condon PI, Fitzgerald G, Burke A, et al: The physical effects of viscoelastic substances on human donor cornea . Trans Ophthalmol Soc UK 1983;103:265-267. 11. Harrison SE, Soll DB, Shayegan M, et al: Chondroitin sulfate: A new and effective protective agent for intraocular lens insertion . Ophthalmology 1982;89:1254-1260.Crossref 12. MacRae SM, Edelhauser HF, Hyndiuk RA, et al: The effects of sodium hyaluronate, chondroitin sulfate, and methylcellulose on the corneal endothelium and intraocular pressure . Am J Ophthalmol 1983;95:332-341. 13. Carty JB: Chondroitin sulfate in anterior segment surgery . Trans Ophthalmol Soc UK 1983; 103:263-264. 14. Ng MC, Riley MV: Relation of intracellular levels and redox state of glutathione to endothelial function in the rabbit cornea . Exp Eye Res 1980;30:511-517.Crossref 15. Edelhauser HF, Van Horn DL, Miller P, et al: Effect of thiol-oxidation of glutathione with diamide on corneal endothelial function, junctional complexes, and microfilaments . J Cell Biol 1976;68:567-578.Crossref 16. Hull DS, Green K, Bowman K, et al: Intracellular pH and glutathione levels in rabbit corneal endothelium following storage in moist chamber and MK medium . Invest Ophthalmol Vis Sci 1983;24:214-217. 17. Trawkin AG, Gundorowa RA, Bordjugowa GG, et al: Erforschung des Zustandes der Zellmembran und der Mechanismen der Hornhautautolyse bei der Konservierung . Klin Monatsbl Augenheilkd 1976;169:500-504. 18. Hull DS, Strickland EC, Green K: Photodynamically induced alteration of cornea endothelial cell function . Invest Ophthalmol Vis Sci 1979;18:1226-1231. 19. Edelhauser HF, Hanneken AM, Pederson HJ, et al: Osmotic tolerance of rabbit and human corneal endothelium . Arch Ophthalmol 1981; 99:1281-1287.Crossref 20. Gonnering R, Edelhauser HF, Van Horn DL, et al: The pH tolerance of rabbit and human corneal endothelium . Invest Ophthalmol Vis Sci 1979;18:373-390.

Journal

Archives of Ophthalmology – American Medical Association

Published: Apr 1, 1986

There are no references for this article.