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Forskolin, a Potent Adenylate Cyclase Activator, Lowers Rabbit Intraocular Pressure

Forskolin, a Potent Adenylate Cyclase Activator, Lowers Rabbit Intraocular Pressure Abstract • To our knowledge, this is the first report of a topically applied, specific adenylate cyclase activator that reduces intraocular pressure. Forskolin, a novel adenylate cyclase activator, is reported to increase cyclic adenosine monophosphate (AMP) in intact cells. Cyclic AMP levels are increased with various antiglaucoma agents. Intraocular pressure in rabbits was significantly reduced with two concentrations of forskolin, 0.1% and 0.05%. Ocular hypertension was noted with 0.5% and 0.2%. No change in IOP was noted with 0.01% forskolin. The IOP lowering with 0.1% and 0.05% forskolin lasted approximately six hours. Mild, transient conjunctival chemosis was noted in the doses that decreased IOP. The contralateral control eye showed decreases in IOP, but the differences between control and tested eyes were significant by the Student's paired t test. References 1. Chunekar KC: Bhav Prakash Nighantu , Hindi translation. Chaukhamba Vidya Bhavan, Banaras, India, 1960, p 86. 2. Sharma PV: Dravya Guna Vigyan: Parts II and III . Chaukhamba Vidaya Bhavan, Banaras, India, 1956, p 273. 3. Linder E, Dohadwalla A, Bhattacharya B: Positive inotropic and blood pressure lowering activity in a diterpene derivative isolated from coleus forskolin: Forskolin . Arzneimittelforsch 1978;28:284-289. 4. Robinson GA, Butcher RW, Sutherland EW: Cyclic AMP . New York, Academic Press Inc, 1971. 5. Metzger H, Linder E: The positive inotropicacting forskolin: A potent adenylatecyclase activator . Arzneimittelforsch 1981;31:1248-1250. 6. Siegl A, Daly J, Smith J: Inhibition of aggregation and stimulation of cyclic AMP generation in intact human platelets by the diterpene forskolin . Mol Pharmacol 1982;21:680-687. 7. Neufeld A, Jampol L, Sears M: Cyclic-AMP in the aqueous humor: The effects of adrenergic agents . Exp Eye Res 1972;14:242-250.Crossref 8. Neufeld A, Sears M: Adenosine 3′,5′monophosphate analogue increases the outflow facility of the primate eye . Invest Ophthalmol Vis Sci 1975;14:688-689. 9. Seamon KB, Padgett W, Daly J: Forskolin: Unique diterpene activator of adenylate cyclase in membranes and in intact cells . Proc Natl Acad Sci USA 1981;78:3363-3367.Crossref 10. Gregory D, Sears M, Bausher L, et al: Intraocular pressure and aqueous flow are decreased by cholera toxin . Invest Ophthalmol Vis Sci 1981;20:371-381. 11. Eakins KE: Prostaglandin and non-prostaglandin mediated breakdown of the blood-aqueous barrier . Exp Eye Res 1977;25( (suppl) ):483-498.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

Forskolin, a Potent Adenylate Cyclase Activator, Lowers Rabbit Intraocular Pressure

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References (12)

Publisher
American Medical Association
Copyright
Copyright © 1984 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1984.01040030124051
Publisher site
See Article on Publisher Site

Abstract

Abstract • To our knowledge, this is the first report of a topically applied, specific adenylate cyclase activator that reduces intraocular pressure. Forskolin, a novel adenylate cyclase activator, is reported to increase cyclic adenosine monophosphate (AMP) in intact cells. Cyclic AMP levels are increased with various antiglaucoma agents. Intraocular pressure in rabbits was significantly reduced with two concentrations of forskolin, 0.1% and 0.05%. Ocular hypertension was noted with 0.5% and 0.2%. No change in IOP was noted with 0.01% forskolin. The IOP lowering with 0.1% and 0.05% forskolin lasted approximately six hours. Mild, transient conjunctival chemosis was noted in the doses that decreased IOP. The contralateral control eye showed decreases in IOP, but the differences between control and tested eyes were significant by the Student's paired t test. References 1. Chunekar KC: Bhav Prakash Nighantu , Hindi translation. Chaukhamba Vidya Bhavan, Banaras, India, 1960, p 86. 2. Sharma PV: Dravya Guna Vigyan: Parts II and III . Chaukhamba Vidaya Bhavan, Banaras, India, 1956, p 273. 3. Linder E, Dohadwalla A, Bhattacharya B: Positive inotropic and blood pressure lowering activity in a diterpene derivative isolated from coleus forskolin: Forskolin . Arzneimittelforsch 1978;28:284-289. 4. Robinson GA, Butcher RW, Sutherland EW: Cyclic AMP . New York, Academic Press Inc, 1971. 5. Metzger H, Linder E: The positive inotropicacting forskolin: A potent adenylatecyclase activator . Arzneimittelforsch 1981;31:1248-1250. 6. Siegl A, Daly J, Smith J: Inhibition of aggregation and stimulation of cyclic AMP generation in intact human platelets by the diterpene forskolin . Mol Pharmacol 1982;21:680-687. 7. Neufeld A, Jampol L, Sears M: Cyclic-AMP in the aqueous humor: The effects of adrenergic agents . Exp Eye Res 1972;14:242-250.Crossref 8. Neufeld A, Sears M: Adenosine 3′,5′monophosphate analogue increases the outflow facility of the primate eye . Invest Ophthalmol Vis Sci 1975;14:688-689. 9. Seamon KB, Padgett W, Daly J: Forskolin: Unique diterpene activator of adenylate cyclase in membranes and in intact cells . Proc Natl Acad Sci USA 1981;78:3363-3367.Crossref 10. Gregory D, Sears M, Bausher L, et al: Intraocular pressure and aqueous flow are decreased by cholera toxin . Invest Ophthalmol Vis Sci 1981;20:371-381. 11. Eakins KE: Prostaglandin and non-prostaglandin mediated breakdown of the blood-aqueous barrier . Exp Eye Res 1977;25( (suppl) ):483-498.Crossref

Journal

Archives of OphthalmologyAmerican Medical Association

Published: Jan 1, 1984

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