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Macrophages in Ocular Tissues of Rats: Determination of Their Number After Local Anaphylaxis and Other Procedures

Macrophages in Ocular Tissues of Rats: Determination of Their Number After Local Anaphylaxis and... Abstract • The number of macrophages in rat ocular tissues was determined, with the tip of the eyelid containing about 3,000 macrophages per cubic millimeter, which is similar to the number of mast cells at this site. Fewer macrophages were present in orbital tissues and conjunctiva. Macrophages accumulated in ocular tissues of immunized rats injected locally with antigen, but the number did not exceed that observed in antigen-injected controls. Injection of various fluids into ocular tissues, but not the trauma of needle punctures alone, stimulated a marked accumulation of macrophages. Thus, the response to nonspecific stimuli masked the macrophage response to antigen-induced, ocular anaphylaxis. References 1. Austen KF: Homeostasis of effector systems which can also be recruited for immunologic reactions . J Immunol 1978;121:793-805. 2. Tannenbaum S, Oertel H, Henderson W, et al: The biologic activity of mast cell granules: I. Elicitation of inflammatory responses in rat skin . J Immunol 1980;125:325-355. 3. Allansmith MR, Baird RS, Henriquez AS, et al: Sequence of mast cell changes in ocular anaphylaxis . Immunology 1983;49:281-287. 4. Yin ET, Galanos C, Kinsky S, et al: Picogram-sensitive assay for endotoxin: Gelation of Limulus polyphemus blood cell lysate induced by purified lipopolysaccharides and lipid A from gram-negative bacteria . Biochim Biophys Acta 1972;261:284-289.Crossref 5. Allansmith MR, Greiner JV, Baird RS: Number of inflammatory cells in the normal conjunctiva . Am J Ophthalmol 1978;86:250-259. 6. Steinman RM, Cohn ZA: The metabolism and physiology of the mononuclear phagocytes , in Zweifach BW, Grant L, McCluskey RT (eds): The Inflammatory Process , ed 2. New York, Academic Press Inc, 1974, pp 449-479. 7. Allansmith MR, Baird RS, Kashima K, et al: Mast cells in ocular tissues of normal rats and rats infected with Nippostrongylus brasiliensis . Invest Ophthalmol Vis Sci 1979;18:863-867. 8. Hill WC: The influence of the cellular infiltrate on the evolution and intensity of delayed hypersensitivity reactions . J Exp Med 1969; 129:363-370.Crossref 9. Chandler JW, Heise ER, Weiser RS: Induction of delayed-type sensitivity-like reactions in the eye by the injection of lymphokines . Invest Ophthalmol 1973;12:400-409. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

Macrophages in Ocular Tissues of Rats: Determination of Their Number After Local Anaphylaxis and Other Procedures

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References (10)

Publisher
American Medical Association
Copyright
Copyright © 1983 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1983.01040020932021
Publisher site
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Abstract

Abstract • The number of macrophages in rat ocular tissues was determined, with the tip of the eyelid containing about 3,000 macrophages per cubic millimeter, which is similar to the number of mast cells at this site. Fewer macrophages were present in orbital tissues and conjunctiva. Macrophages accumulated in ocular tissues of immunized rats injected locally with antigen, but the number did not exceed that observed in antigen-injected controls. Injection of various fluids into ocular tissues, but not the trauma of needle punctures alone, stimulated a marked accumulation of macrophages. Thus, the response to nonspecific stimuli masked the macrophage response to antigen-induced, ocular anaphylaxis. References 1. Austen KF: Homeostasis of effector systems which can also be recruited for immunologic reactions . J Immunol 1978;121:793-805. 2. Tannenbaum S, Oertel H, Henderson W, et al: The biologic activity of mast cell granules: I. Elicitation of inflammatory responses in rat skin . J Immunol 1980;125:325-355. 3. Allansmith MR, Baird RS, Henriquez AS, et al: Sequence of mast cell changes in ocular anaphylaxis . Immunology 1983;49:281-287. 4. Yin ET, Galanos C, Kinsky S, et al: Picogram-sensitive assay for endotoxin: Gelation of Limulus polyphemus blood cell lysate induced by purified lipopolysaccharides and lipid A from gram-negative bacteria . Biochim Biophys Acta 1972;261:284-289.Crossref 5. Allansmith MR, Greiner JV, Baird RS: Number of inflammatory cells in the normal conjunctiva . Am J Ophthalmol 1978;86:250-259. 6. Steinman RM, Cohn ZA: The metabolism and physiology of the mononuclear phagocytes , in Zweifach BW, Grant L, McCluskey RT (eds): The Inflammatory Process , ed 2. New York, Academic Press Inc, 1974, pp 449-479. 7. Allansmith MR, Baird RS, Kashima K, et al: Mast cells in ocular tissues of normal rats and rats infected with Nippostrongylus brasiliensis . Invest Ophthalmol Vis Sci 1979;18:863-867. 8. Hill WC: The influence of the cellular infiltrate on the evolution and intensity of delayed hypersensitivity reactions . J Exp Med 1969; 129:363-370.Crossref 9. Chandler JW, Heise ER, Weiser RS: Induction of delayed-type sensitivity-like reactions in the eye by the injection of lymphokines . Invest Ophthalmol 1973;12:400-409.

Journal

Archives of Ophthalmology – American Medical Association

Published: Dec 1, 1983

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