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Fungal Contamination in Organ Culture

Fungal Contamination in Organ Culture Abstract • Fungal contamination of organ-cultured corneas was studied retrospectively. Over a 3½-year period, 5.2% of 696 corneas became contaminated by fungi in organ culture. The average length of time for fungal contamination to occur in organ culture was 11 days. The most frequent contaminating fungi were those of the Candida species. In addition to previously identified risk factors for donor cornea contamination, those corneas from donors given mechanical respiratory assistance at the time of death, those corneas with long postmortem times, and those corneas with positive postwash cultures were identified as being at higher risk for fungal contamination in organ culture. References 1. Gandhi SS, Lamberts DW, Perry HD: Donor to host transmission of disease via corneal transplantation . Surv Ophthalmol 1981;25:306-311.Crossref 2. White JH: Fungal contamination of donor eyes . Br J Ophthalmol 1969;53:30-33.Crossref 3. Beyt BW, Waltman SR: Cryptococcal endophthalmitis after corneal transplantation . N Engl J Med 1978;298:825-826.Crossref 4. Larsen PA, Lindstrom RL, Doughman DJ: Torulopsis glabrata endophthalmitis after keratoplasty with an organ cultured cornea . Arch Ophthalmol 1978;96:1019-1022.Crossref 5. Doughman DJ: Prolonged donor cornea preservation in organ culture: Long term clinical evaluation . Trans Am Ophthalmol Soc 1980;78:567-628. 6. Williamson J, Gordon AM, Wood R, et al: Fungal flora of the conjunctival sac in health and disease . Br J Ophthalmol 1968;52:127-132.Crossref 7. Polack FM, Locatcher-Khorazo D, Gutierrez E: Bacteriologic study of 'donor' eyes . Arch Ophthalmol 1967;78:219-225.Crossref 8. Rollins HJ, Stocker FW: Bacterial flora and preoperative treatment of donor corneas . Am J Ophthalmol 1965;59:247-249. 9. Armstrong D: Contamination of Tissue Culture by Bacteria and Fungi: Contamination in Tissue Culture . New York, Academic Press Inc, 1973, pp 51-64. 10. O'Day DM, Akrabawi PL, Head WS, et al: Laboratory isolation techniques in human and experimental fungal infections . Am J Ophthalmol 1979;87:5688-5693. 11. Bonner DP, Mechlinski W, Schaffner CP: Stability studies with amphotericin B and amphotericin B methyl ester . J Antibiot 1975;28:132-135.Crossref 12. Cheung SC, Medoff G, Schlessinger D, et al: Stability of amphotericin B in fungal culture media . Antimicrob Agents Chemother 1975;8: 426-428.Crossref 13. Shaw EL, Aquavella JV: Pneumococcal endophthalmitis following grafting of corneal tissue from a (cadaver) kidney donor . Ann Ophthalmol 1977;9:435-440. 14. Binder PS: Eyebanking and Corneal Preservation: Symposium on Medical and Surgical Diseases of the Cornea . St Louis, CV Mosby Co, 1980, pp 320-354. 15. Zakov ZN, Dohlman CH, Perry HD, et al: Corneal donor material selection . Am J Ophthalmol 1978;86:605-610. 16. Wagoner MD, Dohlman CH, Albert DM, et al: Corneal donor material selection . Ophthalmology 1981;88:139-145.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

Fungal Contamination in Organ Culture

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References (18)

Publisher
American Medical Association
Copyright
Copyright © 1983 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1983.01040010282020
Publisher site
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Abstract

Abstract • Fungal contamination of organ-cultured corneas was studied retrospectively. Over a 3½-year period, 5.2% of 696 corneas became contaminated by fungi in organ culture. The average length of time for fungal contamination to occur in organ culture was 11 days. The most frequent contaminating fungi were those of the Candida species. In addition to previously identified risk factors for donor cornea contamination, those corneas from donors given mechanical respiratory assistance at the time of death, those corneas with long postmortem times, and those corneas with positive postwash cultures were identified as being at higher risk for fungal contamination in organ culture. References 1. Gandhi SS, Lamberts DW, Perry HD: Donor to host transmission of disease via corneal transplantation . Surv Ophthalmol 1981;25:306-311.Crossref 2. White JH: Fungal contamination of donor eyes . Br J Ophthalmol 1969;53:30-33.Crossref 3. Beyt BW, Waltman SR: Cryptococcal endophthalmitis after corneal transplantation . N Engl J Med 1978;298:825-826.Crossref 4. Larsen PA, Lindstrom RL, Doughman DJ: Torulopsis glabrata endophthalmitis after keratoplasty with an organ cultured cornea . Arch Ophthalmol 1978;96:1019-1022.Crossref 5. Doughman DJ: Prolonged donor cornea preservation in organ culture: Long term clinical evaluation . Trans Am Ophthalmol Soc 1980;78:567-628. 6. Williamson J, Gordon AM, Wood R, et al: Fungal flora of the conjunctival sac in health and disease . Br J Ophthalmol 1968;52:127-132.Crossref 7. Polack FM, Locatcher-Khorazo D, Gutierrez E: Bacteriologic study of 'donor' eyes . Arch Ophthalmol 1967;78:219-225.Crossref 8. Rollins HJ, Stocker FW: Bacterial flora and preoperative treatment of donor corneas . Am J Ophthalmol 1965;59:247-249. 9. Armstrong D: Contamination of Tissue Culture by Bacteria and Fungi: Contamination in Tissue Culture . New York, Academic Press Inc, 1973, pp 51-64. 10. O'Day DM, Akrabawi PL, Head WS, et al: Laboratory isolation techniques in human and experimental fungal infections . Am J Ophthalmol 1979;87:5688-5693. 11. Bonner DP, Mechlinski W, Schaffner CP: Stability studies with amphotericin B and amphotericin B methyl ester . J Antibiot 1975;28:132-135.Crossref 12. Cheung SC, Medoff G, Schlessinger D, et al: Stability of amphotericin B in fungal culture media . Antimicrob Agents Chemother 1975;8: 426-428.Crossref 13. Shaw EL, Aquavella JV: Pneumococcal endophthalmitis following grafting of corneal tissue from a (cadaver) kidney donor . Ann Ophthalmol 1977;9:435-440. 14. Binder PS: Eyebanking and Corneal Preservation: Symposium on Medical and Surgical Diseases of the Cornea . St Louis, CV Mosby Co, 1980, pp 320-354. 15. Zakov ZN, Dohlman CH, Perry HD, et al: Corneal donor material selection . Am J Ophthalmol 1978;86:605-610. 16. Wagoner MD, Dohlman CH, Albert DM, et al: Corneal donor material selection . Ophthalmology 1981;88:139-145.Crossref

Journal

Archives of Ophthalmology – American Medical Association

Published: Feb 1, 1983

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