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A Permeability Defect of the Retinal Pigment Epithelium: Occurrence in Early Streptozocin Diabetes

A Permeability Defect of the Retinal Pigment Epithelium: Occurrence in Early Streptozocin Diabetes Abstract • The permeability of the blood-retina barrier was tested in rats with early streptozocin-induced diabetes. Two different tracer substances were used: fluorescein sodium and horseradish peroxidase (HRP). After intravenous administration, the ocular distribution of fluorescein was studied by fluorescence microscopy of freeze-dried tissue. A permeability defect of the pigment epithelium to fluorescein was present in one half of the rats four weeks after induction of diabetes. The dye entered the pigment epithelial cells but could not be detected among the photoreceptors. The only dye visible in neural retina was within the retinal blood vessels. For HRP, no fault whatsoever in the blood retina barrier was found: there was no increase of vesicular uptake by the pigment epithelial cells; the tight junctions between pigment epithelial cells were intact as were those between the endothelial cells of retinal blood vessels. References 1. Cuhna-Vaz J, Faria de Abreu JR, Campos AJ, et al: Early breakdown of the blood-retinal barrier in diabetes . Br J Ophthalmol 59:649-656, 1975.Crossref 2. Waltman SR, Oestrich C, Krupin T, et al: Quantitatve vitreous fluorophotometry: A sensitive technique for measuring early breakdown of the blood-retinal barrier in young diabetic patients . Diabetes 27:85-87, 1978.Crossref 3. Grayson MC, Laties AM: Ocular localization of sodium fluorescein . Arch Ophthalmol 85:600-609, 1971.Crossref 4. Graham RC, Karnovsky MJ: The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: Ultrastructural cytochemistry by a new technique . J Histochem Cytochem 14:291-302, 1966.Crossref 5. Greene DA, DeJesus PV, Winegrad AI: Effects of insulin and dietary myoinositol on impaired peripheral motor nerve conduction velocity in acute streptozotocin diabetes . J Clin Invest 55:1326-1336, 1975.Crossref 6. Sherman SH, Green K, Laties AM: The fate of anterior chamber fluorescein in the monkey eye: I. The anterior chamber outflow pathways . Exp Eye Res 27:159-173, 1978.Crossref 7. Cotran RS, Karnovsky MJ: Vascular leakage induced by horseradish peroxidase in the rat . Proc Soc Exp Biol Med 126:557-561, 1967.Crossref 8. Junod A, Lambert AE, Stauffacher W, et al: Diabetogenic action of streptozotocin: Relationship of dose to metabolic response . J Clin Invest 48:2129-2139, 1969.Crossref 9. Brightman MW, Hori M, Rapoport SI, et al: Osmotic opening of tight junctions in cerebral endothelium . J Comp Neurol 152:317-326, 1973.Crossref 10. Grimes P, Laties AM: Early morphological alteration of the pigment epithelium in streptozotocin-induced diabetes: Increased surface area of the basal cell membrane. Read before the Association for Research in Vision and Ophthalmology, Sarasota, Fla, May 2, 1979. Exp Eye Res, to be published. 11. Waltman S, Krupin T, Hanish S, et al: Alteration of the blood-retinal barrier in experimental diabetes mellitus . Arch Ophthalmol 96:878-879, 1978.Crossref 12. Cunha-Vaz J, Maurice D: The active transport of fluorescein by the retinal vessels and the retina . J Physiol 191:467-486, 1967. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

A Permeability Defect of the Retinal Pigment Epithelium: Occurrence in Early Streptozocin Diabetes

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References (13)

Publisher
American Medical Association
Copyright
Copyright © 1980 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1980.01020030719015
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Abstract

Abstract • The permeability of the blood-retina barrier was tested in rats with early streptozocin-induced diabetes. Two different tracer substances were used: fluorescein sodium and horseradish peroxidase (HRP). After intravenous administration, the ocular distribution of fluorescein was studied by fluorescence microscopy of freeze-dried tissue. A permeability defect of the pigment epithelium to fluorescein was present in one half of the rats four weeks after induction of diabetes. The dye entered the pigment epithelial cells but could not be detected among the photoreceptors. The only dye visible in neural retina was within the retinal blood vessels. For HRP, no fault whatsoever in the blood retina barrier was found: there was no increase of vesicular uptake by the pigment epithelial cells; the tight junctions between pigment epithelial cells were intact as were those between the endothelial cells of retinal blood vessels. References 1. Cuhna-Vaz J, Faria de Abreu JR, Campos AJ, et al: Early breakdown of the blood-retinal barrier in diabetes . Br J Ophthalmol 59:649-656, 1975.Crossref 2. Waltman SR, Oestrich C, Krupin T, et al: Quantitatve vitreous fluorophotometry: A sensitive technique for measuring early breakdown of the blood-retinal barrier in young diabetic patients . Diabetes 27:85-87, 1978.Crossref 3. Grayson MC, Laties AM: Ocular localization of sodium fluorescein . Arch Ophthalmol 85:600-609, 1971.Crossref 4. Graham RC, Karnovsky MJ: The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: Ultrastructural cytochemistry by a new technique . J Histochem Cytochem 14:291-302, 1966.Crossref 5. Greene DA, DeJesus PV, Winegrad AI: Effects of insulin and dietary myoinositol on impaired peripheral motor nerve conduction velocity in acute streptozotocin diabetes . J Clin Invest 55:1326-1336, 1975.Crossref 6. Sherman SH, Green K, Laties AM: The fate of anterior chamber fluorescein in the monkey eye: I. The anterior chamber outflow pathways . Exp Eye Res 27:159-173, 1978.Crossref 7. Cotran RS, Karnovsky MJ: Vascular leakage induced by horseradish peroxidase in the rat . Proc Soc Exp Biol Med 126:557-561, 1967.Crossref 8. Junod A, Lambert AE, Stauffacher W, et al: Diabetogenic action of streptozotocin: Relationship of dose to metabolic response . J Clin Invest 48:2129-2139, 1969.Crossref 9. Brightman MW, Hori M, Rapoport SI, et al: Osmotic opening of tight junctions in cerebral endothelium . J Comp Neurol 152:317-326, 1973.Crossref 10. Grimes P, Laties AM: Early morphological alteration of the pigment epithelium in streptozotocin-induced diabetes: Increased surface area of the basal cell membrane. Read before the Association for Research in Vision and Ophthalmology, Sarasota, Fla, May 2, 1979. Exp Eye Res, to be published. 11. Waltman S, Krupin T, Hanish S, et al: Alteration of the blood-retinal barrier in experimental diabetes mellitus . Arch Ophthalmol 96:878-879, 1978.Crossref 12. Cunha-Vaz J, Maurice D: The active transport of fluorescein by the retinal vessels and the retina . J Physiol 191:467-486, 1967.

Journal

Archives of Ophthalmology – American Medical Association

Published: Apr 1, 1980

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