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Alternate and Classical Pathway Components of Complement in the Normal Cornea

Alternate and Classical Pathway Components of Complement in the Normal Cornea Abstract • Activation of complement by either the classical or alternate pathway may be involved in corneal inflammation. This study was undertaken to determine whether the normal human cornea contains components for both classical and alternate pathway activation of complement. Direct immunofluorescence of corneas from human donors using fluorescein-labeled antiserums was used to demonstrate C1q, C3, C4, and C5. The C1q component (the recognition unit of the classical pathway and largest complement component) was found in the periphery of the cornea. Normal donor corneas were also eluted in phosphate-buffered saline at 4 °C for one to four days. Ouchterlony plates, in which the corneal eluate was reacted against antiserums to complement components, disclosed the presence of C1q, C3, C4, C5, properdin, and properdin factor B. Plasminogen was also found. Radial immunodiffusion was used to obtain estimates of the concentrations of C3, C4, and C5 in the cornea. References 1. Roitt IM: Essentials of Immunology . Oxford, Blackwell Scientific Publications, 1977, pp 141-147. 2. Mondino BJ, Rabin BS, Kessler E, et al: Corneal rings with Gram-negative bacteria . Arch Ophthalmol 95:2222-2225, 1977.Crossref 3. Mondino BJ, Brown SI, Rabin BS, et al: Alternate pathway activation of complement in a Proteus mirabilis ulceration of the cornea . Arch Ophthalmol 96:1659-1661, 1978.Crossref 4. Davis NC, West CD, Ho M: Effect of aging of serum on quantitation of complement component C3 . Clin Chem 18:1485-1487, 1972. 5. Allansmith MR, Whitney CR, McClellan BH, et al: Immunoglobulins in the human eye: Location, type, and amount . Arch Ophthalmol 89:36-45, 1973.Crossref 6. Fearon DT, Daha MR, Strom TB, et al: Pathways of complement activation in membranoproliferative glomerulonephritis and allograft rejection . Transplant Proc 9:729-739, 1977. 7. Vogt W: Activation, activities and pharmacologically active products of complement . Pharmacol Rev 26:125-169, 1974. 8. Werb Z, Mainardi CL, Vater CA, et al: Endogenous activation of latent collagenase by rheumatoid synovial cells . N Engl J Med 296:1017-1023, 1977.Crossref 9. Kohler PF, Muller-Eberhard HJ: Immunochemical quantitation of the third, fourth and fifth components of human complement: Concentrations in the serum of healthy adults . J Immunol 99:1211-1216, 1967. 10. Stock EL, Aronson SB: Corneal immune globulin distribution . Arch Ophthalmol 84:355-359, 1970.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

Alternate and Classical Pathway Components of Complement in the Normal Cornea

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References (11)

Publisher
American Medical Association
Copyright
Copyright © 1980 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1980.01020030342023
Publisher site
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Abstract

Abstract • Activation of complement by either the classical or alternate pathway may be involved in corneal inflammation. This study was undertaken to determine whether the normal human cornea contains components for both classical and alternate pathway activation of complement. Direct immunofluorescence of corneas from human donors using fluorescein-labeled antiserums was used to demonstrate C1q, C3, C4, and C5. The C1q component (the recognition unit of the classical pathway and largest complement component) was found in the periphery of the cornea. Normal donor corneas were also eluted in phosphate-buffered saline at 4 °C for one to four days. Ouchterlony plates, in which the corneal eluate was reacted against antiserums to complement components, disclosed the presence of C1q, C3, C4, C5, properdin, and properdin factor B. Plasminogen was also found. Radial immunodiffusion was used to obtain estimates of the concentrations of C3, C4, and C5 in the cornea. References 1. Roitt IM: Essentials of Immunology . Oxford, Blackwell Scientific Publications, 1977, pp 141-147. 2. Mondino BJ, Rabin BS, Kessler E, et al: Corneal rings with Gram-negative bacteria . Arch Ophthalmol 95:2222-2225, 1977.Crossref 3. Mondino BJ, Brown SI, Rabin BS, et al: Alternate pathway activation of complement in a Proteus mirabilis ulceration of the cornea . Arch Ophthalmol 96:1659-1661, 1978.Crossref 4. Davis NC, West CD, Ho M: Effect of aging of serum on quantitation of complement component C3 . Clin Chem 18:1485-1487, 1972. 5. Allansmith MR, Whitney CR, McClellan BH, et al: Immunoglobulins in the human eye: Location, type, and amount . Arch Ophthalmol 89:36-45, 1973.Crossref 6. Fearon DT, Daha MR, Strom TB, et al: Pathways of complement activation in membranoproliferative glomerulonephritis and allograft rejection . Transplant Proc 9:729-739, 1977. 7. Vogt W: Activation, activities and pharmacologically active products of complement . Pharmacol Rev 26:125-169, 1974. 8. Werb Z, Mainardi CL, Vater CA, et al: Endogenous activation of latent collagenase by rheumatoid synovial cells . N Engl J Med 296:1017-1023, 1977.Crossref 9. Kohler PF, Muller-Eberhard HJ: Immunochemical quantitation of the third, fourth and fifth components of human complement: Concentrations in the serum of healthy adults . J Immunol 99:1211-1216, 1967. 10. Stock EL, Aronson SB: Corneal immune globulin distribution . Arch Ophthalmol 84:355-359, 1970.Crossref

Journal

Archives of Ophthalmology – American Medical Association

Published: Feb 1, 1980

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