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Fluorescent Antibody Staining of Treponemes in Uveitis

Fluorescent Antibody Staining of Treponemes in Uveitis Abstract Treponemal organisms isolated from the eyes of three patients with uveitis were examined by ordinary dark field and fluorescent dark field microscopy, the latter following incubation with fluorescein-labelled syphilis antibody globulin. In one patient with congenital syphilis and a reactive fluorescent treponemal antibody-absorbed (FTA-ABS) test in his serum, the organisms did not fluoresce. Two other patients, whose serum showed no evidence of syphilis, also had intraocular treponemes. Specific staining of the organisms occurred in one of these patients and not in the other. The technique of the isolation and staining of intraocular treponemes is described, and the interpretation of the results is discussed. References 1. Argarwal, L.P.: Aqueous Humor Examination in Granulotamous Iridocyclitis , Docum Ophthal 14:97, 1960. 2. Amsler, M.; Verrey, F.; and Huber, A.: L'Humeur Aqueuse et ses Fonctions , Paris: Masson & Cie, 1955. 3. Smith, J.L., and Israel, C.: The Presence of Spirochetes in Late Seronegative Syphilis , JAMA 199:980-984 ( (March 27) ) 1967.Crossref 4. Goldman, J.N., and Girard, K.F.: Intraocular Treponemes in Treated Congenital Syphilis , Arch Ophthal 78:47-50 ( (July) ) 1967.Crossref 5. Wells, J.A., and Smith, J.L.: The Fluorescent Antibody Tissue Stain for Treponema Pallidum in the Eye , Amer J Ophthal 63:410, 1967. 6. Hunter, E.F.; Deacon, W.E.; and Meyer, P.E.: An Improved FTA Test for Syphilis, The Absorption Procedure (FTA-ABS) , Public Health Rep 79:410, 1964.Crossref 7. Deacon, W.E.; Lucas, J.B.; and Price, E.V.: Fluorescent Treponemal Antibody-Absorption (FTA-ABS) Test for Syphilis , JAMA 198:624-628 ( (Nov 7) ) 1966.Crossref 8. Instructions for Use of Lyophilized Fluorescein-Conjugated Anti-T Pallidum Globulin, Atlanta, Venereal Disease Research Laboratory, National Communicable Disease Center, June 15, 1967. 9. Serologic Tests for Syphilis, publication No. 411, Public Health Service, May 1964. 10. Willcox, R.R.: Textbook of Venereal Diseases and Treponematoses , ed 2, Springfield, Ill: Charles C Thomas, Publisher, 1964. 11. Turner, T.B., and Hollander, D.H.: Biology of the Treponematoses , Geneva: World Health Organization, 1957, chap 6 . 12. Collart, P.; Borel, J.L.; and Duvel, P.: Significance of Spiral Organisms Found, After Treatment, in Late Human and Experimental Syphilis , Brit J Venereal Dis 40:81, 1964. 13. Boncinelli, U., et al: Richerche sulla persistenza del Treponema pallidum, nelle linfoghiandole di luetici trattati , G Ital Derm 107:1, 1966. 14. Schuhardt, V.T., and Wilkerson, M.: Relapse Phenomena in Rats Infected With Single Spirochetes (Borrelia recurrentis var turicatae) , J Bact 62:215, 1951. 15. Duke-Elder, S., and Perkins, E.S.: " Diseases of the Uveal Tract ," in System of Ophthalmology , St. Louis: The C. V. Mosby Co. 1966, vol 9. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

Fluorescent Antibody Staining of Treponemes in Uveitis

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References (14)

Publisher
American Medical Association
Copyright
Copyright © 1968 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1968.03850040718010
Publisher site
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Abstract

Abstract Treponemal organisms isolated from the eyes of three patients with uveitis were examined by ordinary dark field and fluorescent dark field microscopy, the latter following incubation with fluorescein-labelled syphilis antibody globulin. In one patient with congenital syphilis and a reactive fluorescent treponemal antibody-absorbed (FTA-ABS) test in his serum, the organisms did not fluoresce. Two other patients, whose serum showed no evidence of syphilis, also had intraocular treponemes. Specific staining of the organisms occurred in one of these patients and not in the other. The technique of the isolation and staining of intraocular treponemes is described, and the interpretation of the results is discussed. References 1. Argarwal, L.P.: Aqueous Humor Examination in Granulotamous Iridocyclitis , Docum Ophthal 14:97, 1960. 2. Amsler, M.; Verrey, F.; and Huber, A.: L'Humeur Aqueuse et ses Fonctions , Paris: Masson & Cie, 1955. 3. Smith, J.L., and Israel, C.: The Presence of Spirochetes in Late Seronegative Syphilis , JAMA 199:980-984 ( (March 27) ) 1967.Crossref 4. Goldman, J.N., and Girard, K.F.: Intraocular Treponemes in Treated Congenital Syphilis , Arch Ophthal 78:47-50 ( (July) ) 1967.Crossref 5. Wells, J.A., and Smith, J.L.: The Fluorescent Antibody Tissue Stain for Treponema Pallidum in the Eye , Amer J Ophthal 63:410, 1967. 6. Hunter, E.F.; Deacon, W.E.; and Meyer, P.E.: An Improved FTA Test for Syphilis, The Absorption Procedure (FTA-ABS) , Public Health Rep 79:410, 1964.Crossref 7. Deacon, W.E.; Lucas, J.B.; and Price, E.V.: Fluorescent Treponemal Antibody-Absorption (FTA-ABS) Test for Syphilis , JAMA 198:624-628 ( (Nov 7) ) 1966.Crossref 8. Instructions for Use of Lyophilized Fluorescein-Conjugated Anti-T Pallidum Globulin, Atlanta, Venereal Disease Research Laboratory, National Communicable Disease Center, June 15, 1967. 9. Serologic Tests for Syphilis, publication No. 411, Public Health Service, May 1964. 10. Willcox, R.R.: Textbook of Venereal Diseases and Treponematoses , ed 2, Springfield, Ill: Charles C Thomas, Publisher, 1964. 11. Turner, T.B., and Hollander, D.H.: Biology of the Treponematoses , Geneva: World Health Organization, 1957, chap 6 . 12. Collart, P.; Borel, J.L.; and Duvel, P.: Significance of Spiral Organisms Found, After Treatment, in Late Human and Experimental Syphilis , Brit J Venereal Dis 40:81, 1964. 13. Boncinelli, U., et al: Richerche sulla persistenza del Treponema pallidum, nelle linfoghiandole di luetici trattati , G Ital Derm 107:1, 1966. 14. Schuhardt, V.T., and Wilkerson, M.: Relapse Phenomena in Rats Infected With Single Spirochetes (Borrelia recurrentis var turicatae) , J Bact 62:215, 1951. 15. Duke-Elder, S., and Perkins, E.S.: " Diseases of the Uveal Tract ," in System of Ophthalmology , St. Louis: The C. V. Mosby Co. 1966, vol 9.

Journal

Archives of Ophthalmology – American Medical Association

Published: Jun 1, 1968

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