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Biochemical Studies of Subretinal Fluid: II. Total Protein and Albumin of Subretinal Fluid and Blood Serum in Patients with Retinal Detachment

Biochemical Studies of Subretinal Fluid: II. Total Protein and Albumin of Subretinal Fluid and... Abstract The protein content of subretinal fluid (SRF) from idiopathic retinal detachment has been of interest since 1893 when Rählmann1 reported a high percentage of albumin and concluded that this fluid must be a transudate from the choroidal vessels. Although for a time this idea became obscured by the popular concept that SRF came from the vitreous through ruptures in the retina, recent work has led to a revival of the transudate theory. In a previous paper,4 we reported ascorbic acid findings together with protein studies which indicated a compound, blood/vitreous source, rather than a single origin, for subretinal fluid. The earlier literature on this controversy has been well reviewed by Magitot2 and Longhena.3 Both of these authors confirmed the observation that SRF contains more protein than does normal vitreous (Magitot, 17 cases; Longhena, 30 cases). Although more recent detachments in their cases tended to have low-protein References 1. This method was tested on dilutions of pooled human serum, and found to be reliable in the range of values found in SRF. 2. The results in patients undergoing more than one surgical procedure will be discussed in a later paper. 3. Rählmann, E.: A Critical Comparison of Leber's Theory of Detachment of the Retina with the Diffusion Theory , Arch. Ophthal. (1st series) 23:92-104, 1894. 4. Magitot, A.: The Subretinal Fluid in Idiopathic Detachment of the Retina , Arch. Ophthal. 11:159-173, 1934.Crossref 5. Longhena, L.: Contribution to the Study of Subretinal Fluid in Retinal Detachment , Ophthalmologica 106:23-40; 80-91, 1943.Crossref 6. Weber, J. C., and Wilson, F. M.: Biochemical Studies of Subretinal Fluid: I. Preliminary Report , Invest. Ophthal. 1:798, 1962. 7. Santoni, A.: Investigation of pH and Refractive Index of Subretinal Fluid in Idiopathic Detachment of the Retina , Rev. Oftal. 1:3-14, 1946 8. abstracted, Excerpta Med. XII 2:6, 1948. 9. Nakano, A.: Studies on Subretinal Fluid: II , Jap. J. Ophthal. 5:23-27, 1961. 10. Dorello, U.: Electrophoretic Research on the Protein Content of the Subretinal Fluid in Idiopathic Detachment of the Retina , Arch. Ottol. 59: 416-422 1955 11. abstracted, Excerpta Med. XII 10: 357, 1956. 12. Ros Pena, R.; Casado Corzo, J. P., and Carreras Matas, B.: Electrophoresis of the Subretinal Fluid , Arch. Soc. Oftal. Hisp.-Amer. 16: 449-454, 1956 13. abstracted in Ophthal. Lit. 10:506, 1957, abstract No. 2885. 14. Miglior, M.; deRosa, C., and Ragnetti, E.: The Chemical Composition of Subretinal Fluid in Idiopathic Retinal Detachment , Ann. Ottol. 85:735-743, 1959 15. abstracted, Ophthal. Lit. 13:798, 1960, abstract No. 4702. 16. Smith, J. L., and Douty, E.: Electrophoresis of Subretinal Fluid , Arch. Ophthal. 64:114-119, 1960.Crossref 17. Manuel, Y.; Royer, J.; Richard, G., and Creyssel, R.: Electrophoresis of Proteins in the Subretinal Fluid in Primary Detachment of the Retina , Ann. Oculist. (Par.) 193:739-751, 1960. 18. Paufique, L.: The Present Status of the Treatment of the Retinal Detachment , Trans. Ophthal. Soc. U.K. 79:221-248, 1959. 19. Balik, J. Z. I.: Electrophoretic Pictures of the Plasma Proteins in Detachment of the Retina , Cesk. Ofthal. 8:363-367, 1952. 20. Baron, J. B.; Guillon, S., and Peyrau, M.: Results of Electrophoresis of Serum in Cataract, Glaucoma, Detachment of the Retina, Iridocyclitis and Retrolental Fibroplasia , Ann. Biol. Chem. (Par.) 13:104-197, 1955. 21. Gemolotto, G.: Electrophoretic Research in the Protein Content in Blood Serum in Some Ocular Diseases: IV. In Idiopathic Detachment of the Retina , Arch. Ottol. 59:511-516, 1955. 22. Watzke, R. C.: Personal communication to the author. 23. Knights, E. M., Jr.; MacDonald, R. P., and Ploomeric, J.: Ultramicro Methods for Clinical Laboratories , New York, Grune & Stratton, 1957. 24. Weber, J. C., and Wilson, F. M.: Unpublished data. 25. Gutman, A. B.; Moore, D. H.; Gutman, E. B.; McClellan, O., and Kabat, E. A.: Fractionation of Serum Proteins in Hyperproteinemia with Special Reference to Multiple Myeloma , J. Clin. Invest. 20:765-783, 1941.Crossref 26. Zimmerman, L. E., and Straatsma, B. R.: Anatomic Relationships of the Retina to the Vitreous Body in Retina Surgery, in 2d Conference of the Retina Foundation , C. L. Schepens, Editor, St. Louis, The C. V. Mosby Company, 1960. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

Biochemical Studies of Subretinal Fluid: II. Total Protein and Albumin of Subretinal Fluid and Blood Serum in Patients with Retinal Detachment

WEBER, JANET C.; WILSON, FRED M.
Archives of Ophthalmology , Volume 69 (3) – Mar 1, 1963
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References (9)

Publisher
American Medical Association
Copyright
Copyright © 1963 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1963.00960040369019
Publisher site
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Abstract

Abstract The protein content of subretinal fluid (SRF) from idiopathic retinal detachment has been of interest since 1893 when Rählmann1 reported a high percentage of albumin and concluded that this fluid must be a transudate from the choroidal vessels. Although for a time this idea became obscured by the popular concept that SRF came from the vitreous through ruptures in the retina, recent work has led to a revival of the transudate theory. In a previous paper,4 we reported ascorbic acid findings together with protein studies which indicated a compound, blood/vitreous source, rather than a single origin, for subretinal fluid. The earlier literature on this controversy has been well reviewed by Magitot2 and Longhena.3 Both of these authors confirmed the observation that SRF contains more protein than does normal vitreous (Magitot, 17 cases; Longhena, 30 cases). Although more recent detachments in their cases tended to have low-protein References 1. This method was tested on dilutions of pooled human serum, and found to be reliable in the range of values found in SRF. 2. The results in patients undergoing more than one surgical procedure will be discussed in a later paper. 3. Rählmann, E.: A Critical Comparison of Leber's Theory of Detachment of the Retina with the Diffusion Theory , Arch. Ophthal. (1st series) 23:92-104, 1894. 4. Magitot, A.: The Subretinal Fluid in Idiopathic Detachment of the Retina , Arch. Ophthal. 11:159-173, 1934.Crossref 5. Longhena, L.: Contribution to the Study of Subretinal Fluid in Retinal Detachment , Ophthalmologica 106:23-40; 80-91, 1943.Crossref 6. Weber, J. C., and Wilson, F. M.: Biochemical Studies of Subretinal Fluid: I. Preliminary Report , Invest. Ophthal. 1:798, 1962. 7. Santoni, A.: Investigation of pH and Refractive Index of Subretinal Fluid in Idiopathic Detachment of the Retina , Rev. Oftal. 1:3-14, 1946 8. abstracted, Excerpta Med. XII 2:6, 1948. 9. Nakano, A.: Studies on Subretinal Fluid: II , Jap. J. Ophthal. 5:23-27, 1961. 10. Dorello, U.: Electrophoretic Research on the Protein Content of the Subretinal Fluid in Idiopathic Detachment of the Retina , Arch. Ottol. 59: 416-422 1955 11. abstracted, Excerpta Med. XII 10: 357, 1956. 12. Ros Pena, R.; Casado Corzo, J. P., and Carreras Matas, B.: Electrophoresis of the Subretinal Fluid , Arch. Soc. Oftal. Hisp.-Amer. 16: 449-454, 1956 13. abstracted in Ophthal. Lit. 10:506, 1957, abstract No. 2885. 14. Miglior, M.; deRosa, C., and Ragnetti, E.: The Chemical Composition of Subretinal Fluid in Idiopathic Retinal Detachment , Ann. Ottol. 85:735-743, 1959 15. abstracted, Ophthal. Lit. 13:798, 1960, abstract No. 4702. 16. Smith, J. L., and Douty, E.: Electrophoresis of Subretinal Fluid , Arch. Ophthal. 64:114-119, 1960.Crossref 17. Manuel, Y.; Royer, J.; Richard, G., and Creyssel, R.: Electrophoresis of Proteins in the Subretinal Fluid in Primary Detachment of the Retina , Ann. Oculist. (Par.) 193:739-751, 1960. 18. Paufique, L.: The Present Status of the Treatment of the Retinal Detachment , Trans. Ophthal. Soc. U.K. 79:221-248, 1959. 19. Balik, J. Z. I.: Electrophoretic Pictures of the Plasma Proteins in Detachment of the Retina , Cesk. Ofthal. 8:363-367, 1952. 20. Baron, J. B.; Guillon, S., and Peyrau, M.: Results of Electrophoresis of Serum in Cataract, Glaucoma, Detachment of the Retina, Iridocyclitis and Retrolental Fibroplasia , Ann. Biol. Chem. (Par.) 13:104-197, 1955. 21. Gemolotto, G.: Electrophoretic Research in the Protein Content in Blood Serum in Some Ocular Diseases: IV. In Idiopathic Detachment of the Retina , Arch. Ottol. 59:511-516, 1955. 22. Watzke, R. C.: Personal communication to the author. 23. Knights, E. M., Jr.; MacDonald, R. P., and Ploomeric, J.: Ultramicro Methods for Clinical Laboratories , New York, Grune & Stratton, 1957. 24. Weber, J. C., and Wilson, F. M.: Unpublished data. 25. Gutman, A. B.; Moore, D. H.; Gutman, E. B.; McClellan, O., and Kabat, E. A.: Fractionation of Serum Proteins in Hyperproteinemia with Special Reference to Multiple Myeloma , J. Clin. Invest. 20:765-783, 1941.Crossref 26. Zimmerman, L. E., and Straatsma, B. R.: Anatomic Relationships of the Retina to the Vitreous Body in Retina Surgery, in 2d Conference of the Retina Foundation , C. L. Schepens, Editor, St. Louis, The C. V. Mosby Company, 1960.

Journal

Archives of Ophthalmology – American Medical Association

Published: Mar 1, 1963

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