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Distribution of Sialic Acids in the Human Eye

Distribution of Sialic Acids in the Human Eye Abstract Histochemical studies suggest the presence of acid mucopolysaccharides in a number of ocular tissues.1-4 Sialic acids have been found in the aqueous and vitreous humors.5 In order to characterize the glycoproteins of other ocular tissues, analyses for sialic acids were made in different parts of the human eye. Normal human eyes obtained at autopsy were provided by the eye bank. The anterior chamber was tapped first to collect the aqueous humor. The cornea was excised, and both epithelial and endothelial surfaces were scraped off. The lens was gently extracted, and its capsule was stripped off. The vitreous was collected in 2 portions: the more liquid anterior part and more viscous posterior fraction. The retina was stripped off the choroid and carefully cleared of adherent vitreous. Each tissue was homogenized separately and heated with 0.1 N H2SO4 at 80 C for 1 hour. The sialic acids References 1. A gift from Dr. Eli Robins, Department of Psychiatry, Washington University School of Medicine. 2. Meyer, K., and Chaffee, E.: The Mucopolysaccharide Acids of the Cornea and Its Enzymatic Hydrolysis , Amer. J. Ophthal. 23:1320, 1940. 3. Meyer, K., and Rapport, M. M.: The Mucopolysaccharides of the Ground Substance of Connective Tissue , Science 113:596, 1951.Crossref 4. Zimmerman, L. E.: Applications of Histochemical Methods for the Demonstration of Acid Mucopolysaccharides in Ophthalmic Pathology , Trans. Amer. Acad. Ophthal. 62:697, 1958. 5. Zimmerman, L. E., and Eastham, A. B.: Acid Mucopolysaccharides in the Retinal Pigment Epithelium and Visual Cell Layer of the Developing Mouse Eye , Amer. J. Ophthal. 47:488 (Jan., (Pt. 2) ) 1959. 6. Balazs, E. A., and Sundblad, L.: Studies on the Structure of the Vitreous Body. V: Soluble Protein Content , J. Biol. Chem. 235:1973, 1960. 7. Warren, L.: The Thiobarbituric Acid Assay of Sialic Acids , J. Biol. Chem. 234:1971, 1959. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

Distribution of Sialic Acids in the Human Eye

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References (6)

Publisher
American Medical Association
Copyright
Copyright © 1961 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1961.01840020565019
Publisher site
See Article on Publisher Site

Abstract

Abstract Histochemical studies suggest the presence of acid mucopolysaccharides in a number of ocular tissues.1-4 Sialic acids have been found in the aqueous and vitreous humors.5 In order to characterize the glycoproteins of other ocular tissues, analyses for sialic acids were made in different parts of the human eye. Normal human eyes obtained at autopsy were provided by the eye bank. The anterior chamber was tapped first to collect the aqueous humor. The cornea was excised, and both epithelial and endothelial surfaces were scraped off. The lens was gently extracted, and its capsule was stripped off. The vitreous was collected in 2 portions: the more liquid anterior part and more viscous posterior fraction. The retina was stripped off the choroid and carefully cleared of adherent vitreous. Each tissue was homogenized separately and heated with 0.1 N H2SO4 at 80 C for 1 hour. The sialic acids References 1. A gift from Dr. Eli Robins, Department of Psychiatry, Washington University School of Medicine. 2. Meyer, K., and Chaffee, E.: The Mucopolysaccharide Acids of the Cornea and Its Enzymatic Hydrolysis , Amer. J. Ophthal. 23:1320, 1940. 3. Meyer, K., and Rapport, M. M.: The Mucopolysaccharides of the Ground Substance of Connective Tissue , Science 113:596, 1951.Crossref 4. Zimmerman, L. E.: Applications of Histochemical Methods for the Demonstration of Acid Mucopolysaccharides in Ophthalmic Pathology , Trans. Amer. Acad. Ophthal. 62:697, 1958. 5. Zimmerman, L. E., and Eastham, A. B.: Acid Mucopolysaccharides in the Retinal Pigment Epithelium and Visual Cell Layer of the Developing Mouse Eye , Amer. J. Ophthal. 47:488 (Jan., (Pt. 2) ) 1959. 6. Balazs, E. A., and Sundblad, L.: Studies on the Structure of the Vitreous Body. V: Soluble Protein Content , J. Biol. Chem. 235:1973, 1960. 7. Warren, L.: The Thiobarbituric Acid Assay of Sialic Acids , J. Biol. Chem. 234:1971, 1959.

Journal

Archives of OphthalmologyAmerican Medical Association

Published: Apr 1, 1961

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