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Thymidine-Tritium Labeling of the Cellular Elements of the Corneal Stroma

Thymidine-Tritium Labeling of the Cellular Elements of the Corneal Stroma Abstract When a corneal transplant "takes," a basic problem remains as to which corneal elements are replaced following the graft. The fate of the sulfated mucopolysaccharides of the transplant has been studied by several investigators using S-351,2 but methods are needed to specifically label and locate individual cells in order to study the movement of the cellular elements. In recent years thymidine-tritium has been used in studying the synthesis of desoxyribonucleic acid (DNA). Once incorporated into the nucleus the radioactive label is retained in the DNA which undergoes very slow turnover during the life of the cell. With highresolution autoradiographic film techniques it is possible to study the subsequent history of the labeled nucleus.3 The use of this method is limited because thymidine is taken up only during the premitotic doubling stage. In the case of the corneal epithelium, where there is rapid proliferation, the labeling problem is not References 1. Dohlman, C. H.: On the Fate of the Corneal Graft , Acta Ophthal. (Kbh.) 35:286, 1957.Crossref 2. LeTessa, A. J.: The Fate of Sulfated Mucopolysaccharides of a Corneal Graft , Ann. N.Y. Acad. Sci. 87:586, 1960.Crossref 3. Hanna, C., and O'Brien, J. E.: Cell Production and Migration in the Epithelial Layer of the Cornea , Arch. Ophthal. 64:536, 1960.Crossref 4. Hanna, C.; Bicknell, D., and O'Brien, J. E.: Cell Turnover in the Adult Human Eye , Arch. Ophthal. 65:695, 1961.Crossref 5. Mills, N. L., and Donn, A.: Incorporation of Tritium-Labeled Thymidine by Rabbit Corneal Endothelium , Arch. Ophthal. 64:443, 1960.Crossref 6. Basu, P. K.: Effect of Different Steroids on the Healing of Nonperforating Corneal Wounds in Rabbits , A.M.A. Arch. Ophthal. 59:657, 1958.Crossref 7. Weimar, V.: The Transformation of Corneal Stromal Cells to Fibroblasts in Corneal Wound Healing , Amer. J. Ophthal. 44:173, 1957. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

Thymidine-Tritium Labeling of the Cellular Elements of the Corneal Stroma

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References (7)

Publisher
American Medical Association
Copyright
Copyright © 1961 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1961.00960010364013
Publisher site
See Article on Publisher Site

Abstract

Abstract When a corneal transplant "takes," a basic problem remains as to which corneal elements are replaced following the graft. The fate of the sulfated mucopolysaccharides of the transplant has been studied by several investigators using S-351,2 but methods are needed to specifically label and locate individual cells in order to study the movement of the cellular elements. In recent years thymidine-tritium has been used in studying the synthesis of desoxyribonucleic acid (DNA). Once incorporated into the nucleus the radioactive label is retained in the DNA which undergoes very slow turnover during the life of the cell. With highresolution autoradiographic film techniques it is possible to study the subsequent history of the labeled nucleus.3 The use of this method is limited because thymidine is taken up only during the premitotic doubling stage. In the case of the corneal epithelium, where there is rapid proliferation, the labeling problem is not References 1. Dohlman, C. H.: On the Fate of the Corneal Graft , Acta Ophthal. (Kbh.) 35:286, 1957.Crossref 2. LeTessa, A. J.: The Fate of Sulfated Mucopolysaccharides of a Corneal Graft , Ann. N.Y. Acad. Sci. 87:586, 1960.Crossref 3. Hanna, C., and O'Brien, J. E.: Cell Production and Migration in the Epithelial Layer of the Cornea , Arch. Ophthal. 64:536, 1960.Crossref 4. Hanna, C.; Bicknell, D., and O'Brien, J. E.: Cell Turnover in the Adult Human Eye , Arch. Ophthal. 65:695, 1961.Crossref 5. Mills, N. L., and Donn, A.: Incorporation of Tritium-Labeled Thymidine by Rabbit Corneal Endothelium , Arch. Ophthal. 64:443, 1960.Crossref 6. Basu, P. K.: Effect of Different Steroids on the Healing of Nonperforating Corneal Wounds in Rabbits , A.M.A. Arch. Ophthal. 59:657, 1958.Crossref 7. Weimar, V.: The Transformation of Corneal Stromal Cells to Fibroblasts in Corneal Wound Healing , Amer. J. Ophthal. 44:173, 1957.

Journal

Archives of OphthalmologyAmerican Medical Association

Published: Sep 1, 1961

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