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Experimental Studies on Cx-Reactive Protein in Rabbit Aqueous

Experimental Studies on Cx-Reactive Protein in Rabbit Aqueous Abstract Preliminary studies (Wolkowicz, Hallett, and Leopold14) on the possible role C-reactive protein might play in ocular pathology suggested that the C-reactive protein (CRP) test, when applied to the aqueous humor, may shed some additional light on the etiology and differential diagnosis of uveitis. The following data are pertinent to the present study. C-reactive protein is found consistently in sera of patients suffering from an active inflammatory or necrotizing process, not so consistently in cases of rheumatoid arthritis, virus infection, and active tuberculosis (Roantree and Rantz10). C-reactive protein, unlike the usual antibody, appears in the early stage of infection, and its titer declines when antibodies tend to appear, during the convalescing period (Perlman et al.9). Comparative electrophoretic studies of CRP and antibody to C-polysaccharide showed that the former seems to migrate with the α-globulin fraction, whereas the latter is found in the γ-globulin group. Perlman et al. conclude References 1. CxRP is a homologous protein appearing in the rabbit blood stream under similar conditions as CRP in the human blood. CxRP designation stems from its ability to precipitate a Cx-polysaccharide in the same manner as the human CRP precipitates the C-polysaccharide. 2. The Schieffelin Company, which supplied us with some of the material used in this study, includes the exact details and directions of the "finger tips technique" in its current CRPA test literature. 3. The herpes virus suspension was grown and prepared by Dr. T. W. Sery, of the Research Laboratory of the Wills Eye Hospital. 4. Foss, B.: Experimental Anaphylactic Iridocyclitis , Acta path. et microbiol. scandinav. , (Supp. 81) , p. 1, 1949. 5. Glaskin, A.; Shubin, H., and Heiken, C. A.: The Value of CRPA (C-Reactive Protein Agent) Compared to Blood Sedimentation Studies in Tuberculosis , Tuberculology 15:62 ( (Dec.) ) 1955. 6. Goldin, M., and Kaplan, M.: A Method for Obtaining Blood for Micro-Test: Application to Determination of C-Reactive Protein and Antistreptolysin-O Titers , Am. J. Clin. Path. 25:1432 ( (Dec. 12) ) 1955. 7. Goldmann, H., and Witmer, R.: Antikörper im Kammerwasser , Ophthalmologica 127:323 ( (April-May) ) 1954.Crossref 8. Gottlieb, P. M.: Bronchial Asthma: A Review of Recent Literature , Ann. Allergy 13:423 ( (July-Aug.) ) 1955. 9. Kaplan, M. A.; Aaronson, A. L.; Henderson, J., and Goldin, M.: C-Reactive Protein Levels and Anti-Streptolysin-O Titers in Bronchial Asthma: A Preliminary Report , Ann. Allergy 13:29 ( (Jan.-Feb.) ) 1955. 10. C-Reactive Protein, editorial , Lancet 1:350 ( (Feb. 13) ) 1954. 11. Maylath, F. R., and Leopold, I. H.: Study of Experimental Intraocular Infection: I. The Recoverability of Organisms Inoculated into Ocular Tissues and Fluids; II. The Influence of Antibiotics and Cortisone, Alone and Combined, on Intraocular Growth of These Organisms , Am. J. Ophth. 40:86 ( (July) ) 1955. 12. Perlman, E.; Bullowa, J. G. M., and Goodkind, R.: An Immunological and Electrophoretic Comparison of Antibody to C-Polysaccharide and the C-Reactive Protein of Acute Phase Serum , J. Exper. Med. 97:110 ( (Feb. 1) ) 1943. 13. Roantree, R. J., and Rantz, L. A.: Clinical Experience with the C-Reactive Protein Test , A. M. A. Arch. Int. Med. 96:674 ( (Nov.) ) 1955.Crossref 14. Stollerman, G. H.; Glick, S. J., and Anderson, H. C.: Effect of Adrenocortical Hormones on Presence of C-Reactive Protein in Blood , Proc. Soc. Exper. Biol. & Med. 87:241 ( (Oct.) ) 1954. 15. Witmer, R.: Zur experimentellen Augentuberculose des Kaninchens: Elektrophorese und Haemagglutination , Ophthalmologica 127:315 ( (April-May) ) 1954. 16. Witmer, R. H.: Experimental Leptospiral Uveitis in Rabbits , A. M. A. Arch. Ophth. 53:547 ( (April) ) 1955. 17. Wolkowicz, M. I.; Hallett, J. W., and Leopold, I. H.: C-Reactive Protein in Ophthalmology: Clinical and Experimental Studies of Its Use , Am. J. Ophth. 41:942 ( (June) ) 1956. 18. Wood, H. F., and McCarty, M.: The Measurement of C-Reactive Protein in Human Sera: Comparison of the Clinical Tests on the Basis of a Quantitative Method , J. Clin. Invest. 30:616 ( (June) ) 1951. 19. Wood, H. F.: The Relationship Between the Acute Phase Response and Antibody Production in the Rabbit: I. Correlation Between the Early Appearance of Cx-Reactive Protein and Subsequent Antibody Production , J. Exper. Med. 98:311 ( (Oct. 1) ) 1953. 20. Wood, H. F.: The Relationship Between the Acute Phase Response and Antibody Production in the Rabbit: II. The Stimulation of CxReactive Protein Response by Certain Adjuvants and the Relation of This Response to the Enhancement of Antibody Formation , J. Exper. Med. 98:321 ( (Oct. 1) ) 1953. 21. Woods, A. C.; Burky, E. L., and Friedenwald, J. S.: Experimental Studies of Ocular Tuberculosis: I. Relation of Ocular Sensitivity to Cutaneous Sensitivity in the Systemically Infected Rabbit , Arch. Ophth. 19:229 ( (Feb.) ) 1938. 22. Woods, A. C.; Burky, E. L., and Friedenwald, J. S.: Experimental Studies of Ocular Tuberculosis: II. Relation of Ocular Activity to Ocular Sensitivity in the Normal Rabbit Infected by Injection of Tubercle Bacilli into the Anterior Chamber , Arch. Ophth. 19:236 ( (Feb.) ) 1938. 23. Woods, A. C.; Burky, E. L., and Friedenwald, J. S.: Experimental Studies of Ocular Tuberculosis: III. Relation of Cutaneous Sensitivity to Ocular Sensitivity in the Normal Rabbit Infected by Injection of Tubercle Bacilli into the Anterior Chamber , Arch. Ophth. 19:245 ( (Feb.) ) 1938. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png A.M.A. Archives of Ophthalmology American Medical Association

Experimental Studies on Cx-Reactive Protein in Rabbit Aqueous

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References (22)

Publisher
American Medical Association
Copyright
Copyright © 1958 American Medical Association. All Rights Reserved.
ISSN
0096-6339
DOI
10.1001/archopht.1958.00940040095011
Publisher site
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Abstract

Abstract Preliminary studies (Wolkowicz, Hallett, and Leopold14) on the possible role C-reactive protein might play in ocular pathology suggested that the C-reactive protein (CRP) test, when applied to the aqueous humor, may shed some additional light on the etiology and differential diagnosis of uveitis. The following data are pertinent to the present study. C-reactive protein is found consistently in sera of patients suffering from an active inflammatory or necrotizing process, not so consistently in cases of rheumatoid arthritis, virus infection, and active tuberculosis (Roantree and Rantz10). C-reactive protein, unlike the usual antibody, appears in the early stage of infection, and its titer declines when antibodies tend to appear, during the convalescing period (Perlman et al.9). Comparative electrophoretic studies of CRP and antibody to C-polysaccharide showed that the former seems to migrate with the α-globulin fraction, whereas the latter is found in the γ-globulin group. Perlman et al. conclude References 1. CxRP is a homologous protein appearing in the rabbit blood stream under similar conditions as CRP in the human blood. CxRP designation stems from its ability to precipitate a Cx-polysaccharide in the same manner as the human CRP precipitates the C-polysaccharide. 2. The Schieffelin Company, which supplied us with some of the material used in this study, includes the exact details and directions of the "finger tips technique" in its current CRPA test literature. 3. The herpes virus suspension was grown and prepared by Dr. T. W. Sery, of the Research Laboratory of the Wills Eye Hospital. 4. Foss, B.: Experimental Anaphylactic Iridocyclitis , Acta path. et microbiol. scandinav. , (Supp. 81) , p. 1, 1949. 5. Glaskin, A.; Shubin, H., and Heiken, C. A.: The Value of CRPA (C-Reactive Protein Agent) Compared to Blood Sedimentation Studies in Tuberculosis , Tuberculology 15:62 ( (Dec.) ) 1955. 6. Goldin, M., and Kaplan, M.: A Method for Obtaining Blood for Micro-Test: Application to Determination of C-Reactive Protein and Antistreptolysin-O Titers , Am. J. Clin. Path. 25:1432 ( (Dec. 12) ) 1955. 7. Goldmann, H., and Witmer, R.: Antikörper im Kammerwasser , Ophthalmologica 127:323 ( (April-May) ) 1954.Crossref 8. Gottlieb, P. M.: Bronchial Asthma: A Review of Recent Literature , Ann. Allergy 13:423 ( (July-Aug.) ) 1955. 9. Kaplan, M. A.; Aaronson, A. L.; Henderson, J., and Goldin, M.: C-Reactive Protein Levels and Anti-Streptolysin-O Titers in Bronchial Asthma: A Preliminary Report , Ann. Allergy 13:29 ( (Jan.-Feb.) ) 1955. 10. C-Reactive Protein, editorial , Lancet 1:350 ( (Feb. 13) ) 1954. 11. Maylath, F. R., and Leopold, I. H.: Study of Experimental Intraocular Infection: I. The Recoverability of Organisms Inoculated into Ocular Tissues and Fluids; II. The Influence of Antibiotics and Cortisone, Alone and Combined, on Intraocular Growth of These Organisms , Am. J. Ophth. 40:86 ( (July) ) 1955. 12. Perlman, E.; Bullowa, J. G. M., and Goodkind, R.: An Immunological and Electrophoretic Comparison of Antibody to C-Polysaccharide and the C-Reactive Protein of Acute Phase Serum , J. Exper. Med. 97:110 ( (Feb. 1) ) 1943. 13. Roantree, R. J., and Rantz, L. A.: Clinical Experience with the C-Reactive Protein Test , A. M. A. Arch. Int. Med. 96:674 ( (Nov.) ) 1955.Crossref 14. Stollerman, G. H.; Glick, S. J., and Anderson, H. C.: Effect of Adrenocortical Hormones on Presence of C-Reactive Protein in Blood , Proc. Soc. Exper. Biol. & Med. 87:241 ( (Oct.) ) 1954. 15. Witmer, R.: Zur experimentellen Augentuberculose des Kaninchens: Elektrophorese und Haemagglutination , Ophthalmologica 127:315 ( (April-May) ) 1954. 16. Witmer, R. H.: Experimental Leptospiral Uveitis in Rabbits , A. M. A. Arch. Ophth. 53:547 ( (April) ) 1955. 17. Wolkowicz, M. I.; Hallett, J. W., and Leopold, I. H.: C-Reactive Protein in Ophthalmology: Clinical and Experimental Studies of Its Use , Am. J. Ophth. 41:942 ( (June) ) 1956. 18. Wood, H. F., and McCarty, M.: The Measurement of C-Reactive Protein in Human Sera: Comparison of the Clinical Tests on the Basis of a Quantitative Method , J. Clin. Invest. 30:616 ( (June) ) 1951. 19. Wood, H. F.: The Relationship Between the Acute Phase Response and Antibody Production in the Rabbit: I. Correlation Between the Early Appearance of Cx-Reactive Protein and Subsequent Antibody Production , J. Exper. Med. 98:311 ( (Oct. 1) ) 1953. 20. Wood, H. F.: The Relationship Between the Acute Phase Response and Antibody Production in the Rabbit: II. The Stimulation of CxReactive Protein Response by Certain Adjuvants and the Relation of This Response to the Enhancement of Antibody Formation , J. Exper. Med. 98:321 ( (Oct. 1) ) 1953. 21. Woods, A. C.; Burky, E. L., and Friedenwald, J. S.: Experimental Studies of Ocular Tuberculosis: I. Relation of Ocular Sensitivity to Cutaneous Sensitivity in the Systemically Infected Rabbit , Arch. Ophth. 19:229 ( (Feb.) ) 1938. 22. Woods, A. C.; Burky, E. L., and Friedenwald, J. S.: Experimental Studies of Ocular Tuberculosis: II. Relation of Ocular Activity to Ocular Sensitivity in the Normal Rabbit Infected by Injection of Tubercle Bacilli into the Anterior Chamber , Arch. Ophth. 19:236 ( (Feb.) ) 1938. 23. Woods, A. C.; Burky, E. L., and Friedenwald, J. S.: Experimental Studies of Ocular Tuberculosis: III. Relation of Cutaneous Sensitivity to Ocular Sensitivity in the Normal Rabbit Infected by Injection of Tubercle Bacilli into the Anterior Chamber , Arch. Ophth. 19:245 ( (Feb.) ) 1938.

Journal

A.M.A. Archives of Ophthalmology – American Medical Association

Published: Mar 1, 1958

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