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GLUTATHIONE IN THE CRYSTALLINE LENS

GLUTATHIONE IN THE CRYSTALLINE LENS Abstract The soluble portion of the lens protein has been the subject of considerable interest and study since the time of Moerner,1 who showed that it was composed almost entirely of the two crystallines which represented in great part the sulphur content. The nitroprusside reaction, which was described by Arnold2 in 1911, showed that the normal lens when treated with a 5 per cent solution of sodium nitroprusside and a drop of ammonia will turn a decided red, resembling the color of permanganate. In studying senile cataract, Reiss3 and Jess4 found that whereas this reaction was positive with the normal lens, it was negative in those parts of the lens that had become cataractous. In mature senile cataract, the red color which was produced ordinarily was entirely absent. Since the positive reaction was said to be caused by the amino-acid cysteine, it was concluded References 1. Moerner, T.: Untersuchung der Proteinsubstanzen in den lichtbrechenden Medien des Auges , Ztschr. f. physiol. Chem. , 1893, vol. 18. 2. Arnold: Eine Farbenreaktion von Eiweisskörpern mit Nitroprussidinatrium: Ueber den Cystein gehalt der tiereschen Organe , Ztschr. f. physiol. Chem. , 1911, vol. 70. 3. Reiss, W.: Ueber die Cysteinreaktion der normal und pathologisch veränderten Linse , Arch. f. Ophth. 80:588, 1911. 4. Jess, A.: Zur Chemie der Cataracta senilis , Arch. f. Augenh. 71:3, 1912. 5. Cohen and Killian: Sulphur Partition of Ocular Humors and Presence of Glutathione in Lenses of the Eye , Proc. Soc. Exper. Biol. & Med. 25:677, 1928. 6. Heffter: Die Reduzierenden Bestandteile der Zelle , Medz. Naturw. Arch. , vol. 1 7. Referiert in Jahresb. ü. d. Fortschr. d. Therchem. 37:565, 1907. 8. Hopkins: Biochem. J. , 1921, vol. 16 9. 1923, vol. 17 10. 1925, vol. 19. 11. Goldschmidt, M. : Beitrag zur Physiologie und Pathologie der Krystallinse , Arch. f. Ophth. , 1917, vol. 93. 12. Michail, D., and Vancea, P.: Sur la teneur en glutathion reduit des tissus oculaires normaux , Compt. rend. Soc. de biol. 99:891, 1928. 13. Perlzwig, W. A., and Delrue, Georges : The Use of the Starch-Iodine End Point in Tunnecliffe's Method for the Determination of Glutathione in Tissues , Biochem. J. 21:1416, 1927. 14. Tunnecliffe: The Occurrence and Quantitative Estimation of Glutathione in Tissues , Biochem. J. 19:194, 1925. 15. Folin, Otto; and Looney, J. M.: Colorimetric Methods for the Separate Determination of Tyrosine, Tryptophane and Cystine in Proteins , J. Biol. Chem. 51:421, 1922. 16. Jess, A., and Kochella, S.: Ueber den Einfluss des Ultravioletten Lichtes auf die Cysteinreaktion der Linse , Arch. f. Ophth. , 1923, vol. 8. 17. Abderhalden and Wertheim: Studien über Autoxydation , Arch. f. d. ges. Physiol. 197:131, 1922. 18. Thompson, J. W., and Voegtlin, Carl: Glutathione Content of Normal Animals , J. Biol. Chem. 70:793, 1926 19. Glutathione Content of Tumor Animals , Thompson J. Biol. Chem. 70:801, 1926. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

GLUTATHIONE IN THE CRYSTALLINE LENS

TASSMAN, I. S.; KARR, W. G.
Archives of Ophthalmology , Volume 2 (4) – Oct 1, 1929
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References (16)

Publisher
American Medical Association
Copyright
Copyright © 1929 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1929.00810020449005
Publisher site
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Abstract

Abstract The soluble portion of the lens protein has been the subject of considerable interest and study since the time of Moerner,1 who showed that it was composed almost entirely of the two crystallines which represented in great part the sulphur content. The nitroprusside reaction, which was described by Arnold2 in 1911, showed that the normal lens when treated with a 5 per cent solution of sodium nitroprusside and a drop of ammonia will turn a decided red, resembling the color of permanganate. In studying senile cataract, Reiss3 and Jess4 found that whereas this reaction was positive with the normal lens, it was negative in those parts of the lens that had become cataractous. In mature senile cataract, the red color which was produced ordinarily was entirely absent. Since the positive reaction was said to be caused by the amino-acid cysteine, it was concluded References 1. Moerner, T.: Untersuchung der Proteinsubstanzen in den lichtbrechenden Medien des Auges , Ztschr. f. physiol. Chem. , 1893, vol. 18. 2. Arnold: Eine Farbenreaktion von Eiweisskörpern mit Nitroprussidinatrium: Ueber den Cystein gehalt der tiereschen Organe , Ztschr. f. physiol. Chem. , 1911, vol. 70. 3. Reiss, W.: Ueber die Cysteinreaktion der normal und pathologisch veränderten Linse , Arch. f. Ophth. 80:588, 1911. 4. Jess, A.: Zur Chemie der Cataracta senilis , Arch. f. Augenh. 71:3, 1912. 5. Cohen and Killian: Sulphur Partition of Ocular Humors and Presence of Glutathione in Lenses of the Eye , Proc. Soc. Exper. Biol. & Med. 25:677, 1928. 6. Heffter: Die Reduzierenden Bestandteile der Zelle , Medz. Naturw. Arch. , vol. 1 7. Referiert in Jahresb. ü. d. Fortschr. d. Therchem. 37:565, 1907. 8. Hopkins: Biochem. J. , 1921, vol. 16 9. 1923, vol. 17 10. 1925, vol. 19. 11. Goldschmidt, M. : Beitrag zur Physiologie und Pathologie der Krystallinse , Arch. f. Ophth. , 1917, vol. 93. 12. Michail, D., and Vancea, P.: Sur la teneur en glutathion reduit des tissus oculaires normaux , Compt. rend. Soc. de biol. 99:891, 1928. 13. Perlzwig, W. A., and Delrue, Georges : The Use of the Starch-Iodine End Point in Tunnecliffe's Method for the Determination of Glutathione in Tissues , Biochem. J. 21:1416, 1927. 14. Tunnecliffe: The Occurrence and Quantitative Estimation of Glutathione in Tissues , Biochem. J. 19:194, 1925. 15. Folin, Otto; and Looney, J. M.: Colorimetric Methods for the Separate Determination of Tyrosine, Tryptophane and Cystine in Proteins , J. Biol. Chem. 51:421, 1922. 16. Jess, A., and Kochella, S.: Ueber den Einfluss des Ultravioletten Lichtes auf die Cysteinreaktion der Linse , Arch. f. Ophth. , 1923, vol. 8. 17. Abderhalden and Wertheim: Studien über Autoxydation , Arch. f. d. ges. Physiol. 197:131, 1922. 18. Thompson, J. W., and Voegtlin, Carl: Glutathione Content of Normal Animals , J. Biol. Chem. 70:793, 1926 19. Glutathione Content of Tumor Animals , Thompson J. Biol. Chem. 70:801, 1926.

Journal

Archives of Ophthalmology – American Medical Association

Published: Oct 1, 1929

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