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[ill]ymphocyte Subpopulations [ill]uring Blood Storage

[ill]ymphocyte Subpopulations [ill]uring Blood Storage Abstract [ill] the Editor.—There is increasing [ill]terest in evaluation, with use of [ill]onoclonal antibodies, of the helper [ill]d suppressor T-lymphocyte sub[ill]pulations in various disease states, [ill]cluding the acquired immune defi[ill]ency syndrome.1,2 Recent studies3,4 [ill]ve emphasized the importance of [ill][ill]operoper processing of blood samples [ill]efore monoclonal-antibody testing [ill]r T-cell subpopulations, to avoid any [ill]isinterpretation of the results that [ill]ight reflect changes occurring be[ill]use of storage. We have investigated [ill]e effect on lymphocyte subpopula[ill]ons produced by different methods of [ill]orage of mononuclear cells before [ill]onoclonal-antibody testing. Peripheral blood from 13 healthy [ill]onors (20 to 50 years old) was drawn [ill] 9 AM and mononuclear cells were [ill]olated on Ficoll-Hypaque. The mono[ill]uclear cells from seven donors were [ill]eparated into five aliquots (Table, [ill]arts A to E). Sample A was tested [ill]mediately after donation; sample B as stored at 22 °C for six hours and [ill]enen at 4 °C for an additional References 1. Ebbesen P, Biggar RJ, Melbye M: AIDS in Europe . Br Med J 1983;287:1324-1326.Crossref 2. Gottlieb MS, Groopman JE, Weinstein WM, et al: The acquired immunodeficiency syndrome . Ann Intern Med 1983;99:208-220.Crossref 3. Dzik WH, Neckers L: Lymphocyte subpopulations altered during blood storage . N Engl J Med 1983;309:435-436. 4. Weiblen BJ, Debell K, Valeri CR: 'Acquired immunodeficiency' of blood stored overnight . N Engl J Med 1983;309:793. 5. Ritchie AWS, Oswald I, Micklem HS, et al: Circadian variation of lymphocyte subpopulations: A study with monoclonal antibodies . Br Med J 1983;286:1773-1775.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Internal Medicine American Medical Association

[ill]ymphocyte Subpopulations [ill]uring Blood Storage

[ill]ymphocyte Subpopulations [ill]uring Blood Storage

Abstract

Abstract [ill] the Editor.—There is increasing [ill]terest in evaluation, with use of [ill]onoclonal antibodies, of the helper [ill]d suppressor T-lymphocyte sub[ill]pulations in various disease states, [ill]cluding the acquired immune defi[ill]ency syndrome.1,2 Recent studies3,4 [ill]ve emphasized the importance of [ill][ill]operoper processing of blood samples [ill]efore monoclonal-antibody testing [ill]r T-cell subpopulations, to avoid any [ill]isinterpretation of the results that...
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References (7)

Publisher
American Medical Association
Copyright
Copyright © 1984 American Medical Association. All Rights Reserved.
ISSN
0003-9926
eISSN
1538-3679
DOI
10.1001/archinte.1984.04400010228051
Publisher site
See Article on Publisher Site

Abstract

Abstract [ill] the Editor.—There is increasing [ill]terest in evaluation, with use of [ill]onoclonal antibodies, of the helper [ill]d suppressor T-lymphocyte sub[ill]pulations in various disease states, [ill]cluding the acquired immune defi[ill]ency syndrome.1,2 Recent studies3,4 [ill]ve emphasized the importance of [ill][ill]operoper processing of blood samples [ill]efore monoclonal-antibody testing [ill]r T-cell subpopulations, to avoid any [ill]isinterpretation of the results that [ill]ight reflect changes occurring be[ill]use of storage. We have investigated [ill]e effect on lymphocyte subpopula[ill]ons produced by different methods of [ill]orage of mononuclear cells before [ill]onoclonal-antibody testing. Peripheral blood from 13 healthy [ill]onors (20 to 50 years old) was drawn [ill] 9 AM and mononuclear cells were [ill]olated on Ficoll-Hypaque. The mono[ill]uclear cells from seven donors were [ill]eparated into five aliquots (Table, [ill]arts A to E). Sample A was tested [ill]mediately after donation; sample B as stored at 22 °C for six hours and [ill]enen at 4 °C for an additional References 1. Ebbesen P, Biggar RJ, Melbye M: AIDS in Europe . Br Med J 1983;287:1324-1326.Crossref 2. Gottlieb MS, Groopman JE, Weinstein WM, et al: The acquired immunodeficiency syndrome . Ann Intern Med 1983;99:208-220.Crossref 3. Dzik WH, Neckers L: Lymphocyte subpopulations altered during blood storage . N Engl J Med 1983;309:435-436. 4. Weiblen BJ, Debell K, Valeri CR: 'Acquired immunodeficiency' of blood stored overnight . N Engl J Med 1983;309:793. 5. Ritchie AWS, Oswald I, Micklem HS, et al: Circadian variation of lymphocyte subpopulations: A study with monoclonal antibodies . Br Med J 1983;286:1773-1775.Crossref

Journal

Archives of Internal MedicineAmerican Medical Association

Published: Oct 1, 1984

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