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SPECTROPHOTOMETRIC DETERMINATION OF BLOOD OXYGEN

SPECTROPHOTOMETRIC DETERMINATION OF BLOOD OXYGEN Abstract Gasometric methods for estimating blood oxygen are time-consuming. This consideration often limits the number of observations which can be made in the course of experiments requiring oxygen determinations. In many cases it is an arteriovenous difference which is particularly desired, as in the measurement of cardiac output by the Fick principle, or the estimation of oxygen consumption by human liver, kidney, or brain. The present paper describes a rapid, simple spectrophotometric method for determination of arteriovenous oxygen differences with an accuracy satisfactory for ordinary needs. The absolute oxygen content can also be estimated, subject to error caused by "inactive" hemoglobin. The method is based upon the principle that two well hemolyzed samples of blood drawn nearly simultaneously from the same individual will have substantially the same total hemoglobin content, "inactive" hemoglobin (1), turbidity, and content of other light-absorbing material; and that, consequently, the difference in optical densities of the two References 1. Ammundsen, E., and Trier, M., Acta med. Scand. , 101, 451 (1939).Crossref 2. Peters, J. P., and Van Slyke, D. D., Quantitative clinical chemistry; Methods , Baltimore, 321 (1932). 3. Drabkin, D. L., and Austin, J. H., J. Biol. Chem. , 112, 105 (1935-36). 4. Drabkin, D. L., and Schmidt, C. F., J. Biol. Chem. , 157, 69 (1945). 5. Hall, F. G., J. Physiol. , 80, 502 (1934). 6. Hall, F. G., J. Biol. Chem. , 130, 573 (1939). 7. Kramer, K., in Abderhalden, E., Handbuch der biologischen Arbeitsmethoden , Berlin and Vienna, Abt. V, Teil 8, 1083 (1935) 8. Brinkman and Wildschut (10). 9. Matthes, K., Arch. exp. Path. u. Pharmakol. , 176, 683 (1934).Crossref 10. Millikan, G. A., J. Physiol. , 79, 152 (1933). 11. Brinkman, R., and Wildschut, A. J. H., Acta med. Scand. , 94, 459 (1938).Crossref 12. Jonxis, J. H. P., Acta med. Scand. , 94, 467 (1938).Crossref 13. Von Issekutz, B., Jr., Arch. exp. Path. u. Pharmakol. , 197, 332 (1940-41).Crossref 14. Lowry, O. H., Smith, C. A., and Cohen, D. L., J. Biol. Chem. , 146, 519 (1942). 15. Groom, D., Wood, E. H., Burchell, H. B., and Parker, R. L., Proc. Staff Meetings Mayo Clin. , 23, 601 (1948). 16. Horecker, B. L., J. Biol. Chem. , 148, 173 (1943). 17. Drabkin, D. L., and Austin, J. H., J. Biol. Chem. , 112, 51 (1935-36). 18. McCarthy, E. F., and Van Slyke, D. D., J. Biol. Chem. , 128, 567 (1939). 19. Lange, H. F., Acta med. Scand. , (suppl.) 176 (1946). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Internal Medicine American Medical Association

SPECTROPHOTOMETRIC DETERMINATION OF BLOOD OXYGEN

HICKAM, J. B.; FRAYSER, R.
Archives of Internal Medicine , Volume 127 (4) – Apr 1, 1971
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References (11)

Publisher
American Medical Association
Copyright
Copyright © 1971 American Medical Association. All Rights Reserved.
ISSN
0003-9926
eISSN
1538-3679
DOI
10.1001/archinte.1971.00310160084007
Publisher site
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Abstract

Abstract Gasometric methods for estimating blood oxygen are time-consuming. This consideration often limits the number of observations which can be made in the course of experiments requiring oxygen determinations. In many cases it is an arteriovenous difference which is particularly desired, as in the measurement of cardiac output by the Fick principle, or the estimation of oxygen consumption by human liver, kidney, or brain. The present paper describes a rapid, simple spectrophotometric method for determination of arteriovenous oxygen differences with an accuracy satisfactory for ordinary needs. The absolute oxygen content can also be estimated, subject to error caused by "inactive" hemoglobin. The method is based upon the principle that two well hemolyzed samples of blood drawn nearly simultaneously from the same individual will have substantially the same total hemoglobin content, "inactive" hemoglobin (1), turbidity, and content of other light-absorbing material; and that, consequently, the difference in optical densities of the two References 1. Ammundsen, E., and Trier, M., Acta med. Scand. , 101, 451 (1939).Crossref 2. Peters, J. P., and Van Slyke, D. D., Quantitative clinical chemistry; Methods , Baltimore, 321 (1932). 3. Drabkin, D. L., and Austin, J. H., J. Biol. Chem. , 112, 105 (1935-36). 4. Drabkin, D. L., and Schmidt, C. F., J. Biol. Chem. , 157, 69 (1945). 5. Hall, F. G., J. Physiol. , 80, 502 (1934). 6. Hall, F. G., J. Biol. Chem. , 130, 573 (1939). 7. Kramer, K., in Abderhalden, E., Handbuch der biologischen Arbeitsmethoden , Berlin and Vienna, Abt. V, Teil 8, 1083 (1935) 8. Brinkman and Wildschut (10). 9. Matthes, K., Arch. exp. Path. u. Pharmakol. , 176, 683 (1934).Crossref 10. Millikan, G. A., J. Physiol. , 79, 152 (1933). 11. Brinkman, R., and Wildschut, A. J. H., Acta med. Scand. , 94, 459 (1938).Crossref 12. Jonxis, J. H. P., Acta med. Scand. , 94, 467 (1938).Crossref 13. Von Issekutz, B., Jr., Arch. exp. Path. u. Pharmakol. , 197, 332 (1940-41).Crossref 14. Lowry, O. H., Smith, C. A., and Cohen, D. L., J. Biol. Chem. , 146, 519 (1942). 15. Groom, D., Wood, E. H., Burchell, H. B., and Parker, R. L., Proc. Staff Meetings Mayo Clin. , 23, 601 (1948). 16. Horecker, B. L., J. Biol. Chem. , 148, 173 (1943). 17. Drabkin, D. L., and Austin, J. H., J. Biol. Chem. , 112, 51 (1935-36). 18. McCarthy, E. F., and Van Slyke, D. D., J. Biol. Chem. , 128, 567 (1939). 19. Lange, H. F., Acta med. Scand. , (suppl.) 176 (1946).

Journal

Archives of Internal Medicine – American Medical Association

Published: Apr 1, 1971

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