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EXPERIMENTS ON THE PROPERTIES OF THE EXTRINSIC FACTOR AND ON THE REACTION OF CASTLE

EXPERIMENTS ON THE PROPERTIES OF THE EXTRINSIC FACTOR AND ON THE REACTION OF CASTLE Abstract The effectiveness of an incubated mixture of meat and normal human gastric juice against anemia was first described by Castle1 in 1929. In the original hypothesis it was assumed that a protein-like substance in the meat was split up by a proteolytic ferment of the normal gastric juice, either in vivo in the normal stomach or in vitro by incubation at 37 C., with formation of the effective antianemic substance. It was assumed by most authors that this newly formed substance was identical with the antianemic factor in liver, although this identity was not expressly stated by Castle. The active factor in normal human gastric juice (called the intrinsic factor) was found to be thermolabile (it was destroyed by a temperature of 60 C. in one-half hour). This intrinsic factor was not identical with either of the known ferments pepsin and rennin. The active factor in the meat (called References 1. Castle, W. B.: Am. J. M. Sc. 178:748, 1939.Crossref 2. Klein, L., and Wilkinson, J. F.: Biochem. J. 28:1684, 1934. 3. Castle, W. B.; Heath, C. W.; Strauss, M. B., and Heinle, R. W.: Am. J. M. Sc. 194:618, 1937.Crossref 4. Wilkinson, J. F.; Klein, L., and Ashford, C. A.: Quart. J. Med. 7:555, 1938. 5. Greenspon, E. A.: Nature of Antipernicious Anemia Principle in Stomach: Method to Improve Stomach Preparations , J. A. M. A. 106:266 ( (Jan. 25) ) 1936.Crossref 6. Taylor, F. H. L.; Castle, W. B.; Heinle, R. W., and Adams, M. A.: J. Clin. Investigation 17:335, 1938.Crossref 7. Uotila, U.: Acta med. Scandinav. 95:415, 1938.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Internal Medicine American Medical Association

EXPERIMENTS ON THE PROPERTIES OF THE EXTRINSIC FACTOR AND ON THE REACTION OF CASTLE

Archives of Internal Medicine , Volume 66 (6) – Dec 1, 1940

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References (2)

Publisher
American Medical Association
Copyright
Copyright © 1940 American Medical Association. All Rights Reserved.
ISSN
0730-188X
DOI
10.1001/archinte.1940.00190180003001
Publisher site
See Article on Publisher Site

Abstract

Abstract The effectiveness of an incubated mixture of meat and normal human gastric juice against anemia was first described by Castle1 in 1929. In the original hypothesis it was assumed that a protein-like substance in the meat was split up by a proteolytic ferment of the normal gastric juice, either in vivo in the normal stomach or in vitro by incubation at 37 C., with formation of the effective antianemic substance. It was assumed by most authors that this newly formed substance was identical with the antianemic factor in liver, although this identity was not expressly stated by Castle. The active factor in normal human gastric juice (called the intrinsic factor) was found to be thermolabile (it was destroyed by a temperature of 60 C. in one-half hour). This intrinsic factor was not identical with either of the known ferments pepsin and rennin. The active factor in the meat (called References 1. Castle, W. B.: Am. J. M. Sc. 178:748, 1939.Crossref 2. Klein, L., and Wilkinson, J. F.: Biochem. J. 28:1684, 1934. 3. Castle, W. B.; Heath, C. W.; Strauss, M. B., and Heinle, R. W.: Am. J. M. Sc. 194:618, 1937.Crossref 4. Wilkinson, J. F.; Klein, L., and Ashford, C. A.: Quart. J. Med. 7:555, 1938. 5. Greenspon, E. A.: Nature of Antipernicious Anemia Principle in Stomach: Method to Improve Stomach Preparations , J. A. M. A. 106:266 ( (Jan. 25) ) 1936.Crossref 6. Taylor, F. H. L.; Castle, W. B.; Heinle, R. W., and Adams, M. A.: J. Clin. Investigation 17:335, 1938.Crossref 7. Uotila, U.: Acta med. Scandinav. 95:415, 1938.Crossref

Journal

Archives of Internal MedicineAmerican Medical Association

Published: Dec 1, 1940

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