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MATERIAL FROM LYMPH NODES OF MAN: II. STUDIES ON LIVING AND FIXED CELLS WITHDRAWN FROM LYMPH NODES OF MAN

MATERIAL FROM LYMPH NODES OF MAN: II. STUDIES ON LIVING AND FIXED CELLS WITHDRAWN FROM LYMPH... Abstract In a previous paper I described a simple method for obtaining living cells from lymph nodes or tumors. Particular stress has been placed on the study of living cells, because one not only obtains from them a true cytologic picture, but also can observe them in their physiologic behavior to altered environmental conditions. Apparently most of the work on the pathology of lymph nodes has been concerned chiefly with their altered architecture and their grosser histology. Dr. George R. Minot suggested to me that a study of the fresh cells of lymph nodes might be worth while, and from former work I believed that a closer scrutiny of cell organs, such as mitochondria specific granules, segregation bodies and other cytoplasmic and nuclear structures, not only in fixed preparations, but also with special reference to the pathologic physiology and histology of these organs in living cells, might provide valuable information. It References 1. Forkner, C. E.: A Method to Obtain Material by Puncture of Lymph Nodes for Study with Supravital and Fixed Stains , Arch. Int. Med. , p. 532, this issue. 2. Some of these cases were studied at the New England Deaconess and New England Baptist Hospitals. 3. The figures which accompany this paper have in some instances been made from fixed preparations rather than from supravitally stained ones. This is because it was often impossible to secure the services of the artist when the living cells had just been withdrawn, which is the best time to observe them. 4. Sabin, F. R.: Studies on Living Human Blood Cells , Bull Johns Hopkins Hosp. 34:277, 1923. 5. Cunningham, R. S.; Sabin, F. R., and Doan, C. A.: The Development of Leucocytes, Lymphocytes, and Monocytes from a Specific Stem-Cell in Adult Tissues , pub. 361, Contribution to Embryology, 84 Carnegie Inst., Washington, 1925, p. 227. 6. McJunkin, F. A.: Origin of Mononuclear Phagocytes of Peritoneal Exudates , Am. J. Path. 1:305, 1925. 7. When a diagnosis was rendered in a name different from that used in this paper it has been given here in the terminology adhered to in this communication. 8. Excellent descriptions of living blood cells stained by the supravital method are given in the articles of Sabin and Simpson referred to in the bibliography of this and the preceding paper. 9. Sternberg, C.: Universelle Primärerkrankungen des lymphatischen Apparates , Centralbl. f. d. Grenzgeb. d. Med. u. Chir. 2:641, 1899 10. Ueber eine eigenartige unter dem Bild der Pseudoleukämie verlaufende Tuberculose des lymphatischen Apparates , Ztschr. f. Heidk. 19:21, 1896. 11. Reed, D. M.: On Pathological Changes in Hodgkin's Disease, with Especial Reference to its Relation to Tuberculosis , Johns Hopkins Hosp. Rep. 10:133, 1902. 12. Longcope, W. T.: Pathological Histology of Hodgkin's Disease, with a Report of a Series of Cases . Bull. Ayer Clin. Lab. 1:4, 1903. 13. Mallory, Frank B.: Principles of Pathologic Histology , Philadelphia, W. B. Saunders Company, 1915. 14. McJunkin, F. A.: Supravital Reaction to Neutral Red of the Cells of Lymph Nodes of Hodgkin's Disease , Arch. Path. 2:815 ( (Dec.) ) 1926. 15. White, W. C., and proscher, F.: Spirochaetes in Acute Lymphatic Leukemia and in Chronic Benign Lymphomatosis , J. A. M. A. 49:1115 ( (Sept. 28) ) 1907.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Internal Medicine American Medical Association

MATERIAL FROM LYMPH NODES OF MAN: II. STUDIES ON LIVING AND FIXED CELLS WITHDRAWN FROM LYMPH NODES OF MAN

Archives of Internal Medicine , Volume 40 (5) – Nov 1, 1927

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References (11)

Publisher
American Medical Association
Copyright
Copyright © 1927 American Medical Association. All Rights Reserved.
ISSN
0730-188X
DOI
10.1001/archinte.1927.00130110075007
Publisher site
See Article on Publisher Site

Abstract

Abstract In a previous paper I described a simple method for obtaining living cells from lymph nodes or tumors. Particular stress has been placed on the study of living cells, because one not only obtains from them a true cytologic picture, but also can observe them in their physiologic behavior to altered environmental conditions. Apparently most of the work on the pathology of lymph nodes has been concerned chiefly with their altered architecture and their grosser histology. Dr. George R. Minot suggested to me that a study of the fresh cells of lymph nodes might be worth while, and from former work I believed that a closer scrutiny of cell organs, such as mitochondria specific granules, segregation bodies and other cytoplasmic and nuclear structures, not only in fixed preparations, but also with special reference to the pathologic physiology and histology of these organs in living cells, might provide valuable information. It References 1. Forkner, C. E.: A Method to Obtain Material by Puncture of Lymph Nodes for Study with Supravital and Fixed Stains , Arch. Int. Med. , p. 532, this issue. 2. Some of these cases were studied at the New England Deaconess and New England Baptist Hospitals. 3. The figures which accompany this paper have in some instances been made from fixed preparations rather than from supravitally stained ones. This is because it was often impossible to secure the services of the artist when the living cells had just been withdrawn, which is the best time to observe them. 4. Sabin, F. R.: Studies on Living Human Blood Cells , Bull Johns Hopkins Hosp. 34:277, 1923. 5. Cunningham, R. S.; Sabin, F. R., and Doan, C. A.: The Development of Leucocytes, Lymphocytes, and Monocytes from a Specific Stem-Cell in Adult Tissues , pub. 361, Contribution to Embryology, 84 Carnegie Inst., Washington, 1925, p. 227. 6. McJunkin, F. A.: Origin of Mononuclear Phagocytes of Peritoneal Exudates , Am. J. Path. 1:305, 1925. 7. When a diagnosis was rendered in a name different from that used in this paper it has been given here in the terminology adhered to in this communication. 8. Excellent descriptions of living blood cells stained by the supravital method are given in the articles of Sabin and Simpson referred to in the bibliography of this and the preceding paper. 9. Sternberg, C.: Universelle Primärerkrankungen des lymphatischen Apparates , Centralbl. f. d. Grenzgeb. d. Med. u. Chir. 2:641, 1899 10. Ueber eine eigenartige unter dem Bild der Pseudoleukämie verlaufende Tuberculose des lymphatischen Apparates , Ztschr. f. Heidk. 19:21, 1896. 11. Reed, D. M.: On Pathological Changes in Hodgkin's Disease, with Especial Reference to its Relation to Tuberculosis , Johns Hopkins Hosp. Rep. 10:133, 1902. 12. Longcope, W. T.: Pathological Histology of Hodgkin's Disease, with a Report of a Series of Cases . Bull. Ayer Clin. Lab. 1:4, 1903. 13. Mallory, Frank B.: Principles of Pathologic Histology , Philadelphia, W. B. Saunders Company, 1915. 14. McJunkin, F. A.: Supravital Reaction to Neutral Red of the Cells of Lymph Nodes of Hodgkin's Disease , Arch. Path. 2:815 ( (Dec.) ) 1926. 15. White, W. C., and proscher, F.: Spirochaetes in Acute Lymphatic Leukemia and in Chronic Benign Lymphomatosis , J. A. M. A. 49:1115 ( (Sept. 28) ) 1907.Crossref

Journal

Archives of Internal MedicineAmerican Medical Association

Published: Nov 1, 1927

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