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19-DEJ-1, a Monoclonal Antibody to the Hemidesmosome-Anchoring Filament Complex, Is the Only Reliable Immunohistochemical Probe for All Major Forms of Junctional Epidermolysis Bullosa

19-DEJ-1, a Monoclonal Antibody to the Hemidesmosome-Anchoring Filament Complex, Is the Only... Abstract • Recently, a monocional antibody, 19-DEJ-1, has been described with binding specificity for an epitope present within the mid-lamina lucida of the dermoepidermal junction directly underneath hemidesmosomes, suggesting recognition of a portion of the anchoring filaments. In an initial survey of specimens from patients with inherited epidermolysis bullosa (EB), it was noted that 9 of 9 specimens of Herlitz junctional EB and approximately 50% of specimens of recessive dystrophic EB lacked staining of the dermoepidermal junction with this antibody. To better define the sensitivity of binding by 19-DEJ-1 in junctional EB skin, 40 consecutive specimens representing the three major subtypes (Herlitz, 14; non-Herlitz, 15; and indeterminate, 11) were examined. No staining was noted along the dermoepidermal junction in any specimen, regardless of junctional EB subtype. Considering the recently discovered variability of binding by GB3 monoclonal antibody in some junctional EB subtypes, based on the present data it appears that the 19-DEJ-1 monoclonal antibody is the only immunohistochemical probe that can be used reliably for diagnosis of all major forms of junctional EB. Furthermore, these data suggest the possible utility of this particular antibody as a probe for identification, at the molecular level, of a basement membrane defect shared among all major forms of junctional EB. (Arch Dermatol. 1990;126:1187-1190) References 1. Foidart J-M, Bere EW, Yaar M, et al. Distribution and immunoelectron microscopic localization of laminin, a non-collagenous basement membrane glycoprotein . Lab Invest. 1980;42:336-342. 2. Bentz H, Morris NP, Murray LW, et al. Isolation and partial characterization of a new human collagen with an extended triplehelical structural domain . Proc Natl Acad Sci U S A. 1983;80:3168-3172,Crossref 3. Fine JD, Horiguchi Y, Madri JA. Ultrastructural localization of AB2 type V collagen in human skin basement membrane and its expression in epidermolysis bullosa . Clin Res. 1989;37:358A. 4. Keene DR, Sakai Y, Lunstrum GP, Morris NP, Burgeson RE. Type VII collagen forms an extended network of anchoring fibrils . J Cell Biol. 1987;104:611-621.Crossref 5. Leigh IM, Eady RAJ, Heagerty AHM, Purkis P, Whitehead PA, Burgeson RE. Type VII collagen is a normal component of epidermal basement membrane which shows altered expression in recessive dystrophic epidermolysis bullosa . J Invest Dermatol. 1988;90:639-642.Crossref 6. Sakai LY, Keene DR, Morris NP, Burgeson RE. Type VII collagen is a major structural component of anchoring fibrils . J Cell Biol. 1986;103:1577-1586.Crossref 7. Woodley DT, Scheidt VJ, Reese MJ, et al. Localization of the alpha 3 (V) chain of type V collagen in human skin . J Invest Dermatol. 1987;88:246-252.Crossref 8. Yaoita H, Foidart J-M, Katz S. Localization of the collagenous component in skin basement membrane . J Invest Dermatol. 1978;70:191-193.Crossref 9. Stenn KS, Madri JA, Roll FJ. Migrating epidermis produces AB2 collagen and requires continuous collagen synthesis for movement . Nature . 1979;277:229-232.Crossref 10. Couchman JR. Heterogeneous distribution of a basement membrane heparan sulfate proteoglycan in rat tissues . J Cell Biol. 1987;105:1901-1916.Crossref 11. Caughman SW, Krieg T, Timpl R, Hintner H, Katz SI. Nidogen and heparan sulfate proteoglycan: detection of newly isolated basement membrane components in normal and epidermolysis bullosa skin . J Invest Dermatol. 1987;89:547-550.Crossref 12. Fine JD, Couchman JR. Chondroitin-6-sulfate-containing proteoglycan: a new component of human skin dermoepidermal junction . J Invest Dermatol. 1988;90:283-288.Crossref 13. Horiguchi Y, Couchman JR, Ljubimov AV, Yamasaki H, Fine J-D. Distribution, ultrastructural localization, and ontogeny of the core protein of a heparan sulfate proteoglycan in human skin and other basement membranes . J Histochem Cytochem. 1989;37:961-970.Crossref 14. Carlin B, Jaffe R, Bender B, Chung AE. Entactin: a novel basal lamina-associated sulfated glycoprotein . J Biol Chem. 1979;256:5209-5214. 15. Horiguchi Y, Fine JD, Ljubimov AV, Yamasaki H, Couchman JR. Entactin: ultrastructural localization of a ubiquitous basement membrane glycoprotein in mouse skin . Arch Dermatol Res. 1989;281:427-432.Crossref 16. Timpl R, Dziadek M, Fujiwara S, Nowack H, Wick G. Nidogen: a new, self-aggregating basement membrane protein . Eur J Biochem. 1983;137:455-465.Crossref 17. Goldsmith LA, Briggaman RA. Monoclonal antibodies to anchoring fibrils for the diagnosis of epidermolysis bullosa . J Invest Dermatol. 1983;81:464-466.Crossref 18. Verrando P, Ortonne J-P, Pautrat G, Hsi B-L, Yeh C-J. Identification of a 37 kilodalton protein at the epidermal basement membrane by anti-serum to human amnion . J Invest Dermatol. 1986;86:190-196.Crossref 19. Fine JD, Gay S. A ubiquitous non-collagenous lamina densa component of basement membrane detected by monoclonal antibody technique . J Invest Dermatol. 1986;86:286-289.Crossref 20. Verrando P, Pisani A, Ortonne J-P. The new basement membrane antigen recognized by the monoclonal antibody GB3 is a large size glycoprotein: modulation of its expression by retinoic acid . Biochim Biophys Acta. 1988;942:45-56.Crossref 21. Verrando P, Hsi B-L, Yeh C-J, Pisano A, Serleys N, Ortonne J-P. Monoclonal antibody GB3: a new probe for the study of human basement membranes and hemidesmosomes . Exp Cell Res. 1987;170:116-128.Crossref 22. Fine JD, Horiguchi Y, Jester J, Couchman J. Detection and partial characterization of a mid-lamina lucida-hemidesmosome-associated antigen (19-DEJ-1) present within human skin . J Invest Dermatol. 1989;92:825-830.Crossref 23. Eady RAJ, Tidman MJ, Heagerty AHM, Kennedy AR. Approaches to the study of epidermolysis bullosa . Curr Probl Dermatol. 1987;17:127-141. 24. Fine JD. Altered skin basement membrane antigenicity in epidermolysis bullosa . In: Wuepper KD, Gedde-Dahl T Jr, eds. Biology of Heritable Skin Diseases . New York, NY: S Karger AG; 1987:111-126. 25. Fine JD. Antigenic features and structural correlates of basement membranes: relationship to epidermolysis bullosa . Arch Dermatol. 1988;124:713-717.Crossref 26. Kennedy AR, Heagerty AHM, Ortonne J-P, Hsi B-L, Yeh C-JG, Eady RAJ. Abnormal binding of an anti-amnion antibody to epidermal basement membrane provides a novel diagnostic probe for junctional epidermolysis bullosa . Br J Dermatol. 1985;113:651-659.Crossref 27. Heagerty AHM, Kennedy AR, Eady RAJ, et al. GB3 monoclonal antibody for diagnosis of junctional epidermolysis bullosa . Lancet. 1986;1:860.Crossref 28. Heagerty AHM, Eady RAJ, Kennedy AR, et al. Rapid prenatal diagnosis of epidermolysis bullosa letalis using GB3 monoclonal antibody . Br J Dermatol. 1987;117:271-275.Crossref 29. Heagerty AHM, Kennedy AR, Gunner DB, Eady RAJ. Rapid prenatal diagnosis and exclusion of epidermolysis bullosa using novel antibody probes . J Invest Dermatol. 1986;86:603-605.Crossref 30. Fine JD. GB3 antigen is an accurate marker of only the Herlitz subset of junctional epidermolysis bullosa . Clin Res. 1990; 38:72A. 31. Fine JD, Horiguchi Y, Couchman JR. 19-DEJ-1, a hemidesmosome-anchoring filament complex-associated monoclonal antibody: definition of a new skin basement membrane antigenic defect in junctional and dystrophic epidermolysis bullosa . Arch Dermatol. 1989;125:520-523.Crossref 32. Hintner H, Stingl G, Schuler G, et al. Immunofluorescence mapping of antigenic determinants within the dermal-epidermal junction in mechanobullous diseases . J Invest Dermatol. 1981; 76:113-118.Crossref 33. Regnier M, Vaigot P, Michel S, Prunieras M. Localization of bullous pemphigoid antigen (BPA) in isolated human keratinocytes . J Invest Dermatol. 1985;85:187-190.Crossref 34. Mutasim DF, Takahashi Y, Labib RS, Anhalt GJ, Patel HP, Diaz LA. A pool of bullous pemphigoid antigen(s) is intracellular and associated with basal cell cytoskeleton-hemidesmosome complex . J Invest Dermatol. 1985;84:47-53.Crossref 35. Westgate GE, Weaver AC, Couchman JR. Bullous pemphigoid antigen localization suggests an intracellur association with hemidesomosomes . J Invest Dermatol. 1985;84:218-224.Crossref 36. Tidman MJ, Eady RAJ. Hemidesmosomes heterogeneity in junctional epidermolysis bullosa revealed by morphometric analysis . J Invest Dermatol. 1986;86:51-56.Crossref 37. Lunstrum GP, Rousselle P, Keene DR, Burgeson RE. Identification of a new basement membrane antigen which localizes to the lamina lucida and displays a novel tissue distribution . J Cell Biol. 1989;109:15A. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Dermatology American Medical Association

19-DEJ-1, a Monoclonal Antibody to the Hemidesmosome-Anchoring Filament Complex, Is the Only Reliable Immunohistochemical Probe for All Major Forms of Junctional Epidermolysis Bullosa

Archives of Dermatology , Volume 126 (9) – Sep 1, 1990

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References (39)

Publisher
American Medical Association
Copyright
Copyright © 1990 American Medical Association. All Rights Reserved.
ISSN
0003-987X
eISSN
1538-3652
DOI
10.1001/archderm.1990.01670330067008
Publisher site
See Article on Publisher Site

Abstract

Abstract • Recently, a monocional antibody, 19-DEJ-1, has been described with binding specificity for an epitope present within the mid-lamina lucida of the dermoepidermal junction directly underneath hemidesmosomes, suggesting recognition of a portion of the anchoring filaments. In an initial survey of specimens from patients with inherited epidermolysis bullosa (EB), it was noted that 9 of 9 specimens of Herlitz junctional EB and approximately 50% of specimens of recessive dystrophic EB lacked staining of the dermoepidermal junction with this antibody. To better define the sensitivity of binding by 19-DEJ-1 in junctional EB skin, 40 consecutive specimens representing the three major subtypes (Herlitz, 14; non-Herlitz, 15; and indeterminate, 11) were examined. No staining was noted along the dermoepidermal junction in any specimen, regardless of junctional EB subtype. Considering the recently discovered variability of binding by GB3 monoclonal antibody in some junctional EB subtypes, based on the present data it appears that the 19-DEJ-1 monoclonal antibody is the only immunohistochemical probe that can be used reliably for diagnosis of all major forms of junctional EB. Furthermore, these data suggest the possible utility of this particular antibody as a probe for identification, at the molecular level, of a basement membrane defect shared among all major forms of junctional EB. (Arch Dermatol. 1990;126:1187-1190) References 1. Foidart J-M, Bere EW, Yaar M, et al. Distribution and immunoelectron microscopic localization of laminin, a non-collagenous basement membrane glycoprotein . Lab Invest. 1980;42:336-342. 2. Bentz H, Morris NP, Murray LW, et al. Isolation and partial characterization of a new human collagen with an extended triplehelical structural domain . Proc Natl Acad Sci U S A. 1983;80:3168-3172,Crossref 3. Fine JD, Horiguchi Y, Madri JA. Ultrastructural localization of AB2 type V collagen in human skin basement membrane and its expression in epidermolysis bullosa . Clin Res. 1989;37:358A. 4. Keene DR, Sakai Y, Lunstrum GP, Morris NP, Burgeson RE. Type VII collagen forms an extended network of anchoring fibrils . J Cell Biol. 1987;104:611-621.Crossref 5. Leigh IM, Eady RAJ, Heagerty AHM, Purkis P, Whitehead PA, Burgeson RE. Type VII collagen is a normal component of epidermal basement membrane which shows altered expression in recessive dystrophic epidermolysis bullosa . J Invest Dermatol. 1988;90:639-642.Crossref 6. Sakai LY, Keene DR, Morris NP, Burgeson RE. Type VII collagen is a major structural component of anchoring fibrils . J Cell Biol. 1986;103:1577-1586.Crossref 7. Woodley DT, Scheidt VJ, Reese MJ, et al. Localization of the alpha 3 (V) chain of type V collagen in human skin . J Invest Dermatol. 1987;88:246-252.Crossref 8. Yaoita H, Foidart J-M, Katz S. Localization of the collagenous component in skin basement membrane . J Invest Dermatol. 1978;70:191-193.Crossref 9. Stenn KS, Madri JA, Roll FJ. Migrating epidermis produces AB2 collagen and requires continuous collagen synthesis for movement . Nature . 1979;277:229-232.Crossref 10. Couchman JR. Heterogeneous distribution of a basement membrane heparan sulfate proteoglycan in rat tissues . J Cell Biol. 1987;105:1901-1916.Crossref 11. Caughman SW, Krieg T, Timpl R, Hintner H, Katz SI. Nidogen and heparan sulfate proteoglycan: detection of newly isolated basement membrane components in normal and epidermolysis bullosa skin . J Invest Dermatol. 1987;89:547-550.Crossref 12. Fine JD, Couchman JR. Chondroitin-6-sulfate-containing proteoglycan: a new component of human skin dermoepidermal junction . J Invest Dermatol. 1988;90:283-288.Crossref 13. Horiguchi Y, Couchman JR, Ljubimov AV, Yamasaki H, Fine J-D. Distribution, ultrastructural localization, and ontogeny of the core protein of a heparan sulfate proteoglycan in human skin and other basement membranes . J Histochem Cytochem. 1989;37:961-970.Crossref 14. Carlin B, Jaffe R, Bender B, Chung AE. Entactin: a novel basal lamina-associated sulfated glycoprotein . J Biol Chem. 1979;256:5209-5214. 15. Horiguchi Y, Fine JD, Ljubimov AV, Yamasaki H, Couchman JR. Entactin: ultrastructural localization of a ubiquitous basement membrane glycoprotein in mouse skin . Arch Dermatol Res. 1989;281:427-432.Crossref 16. Timpl R, Dziadek M, Fujiwara S, Nowack H, Wick G. Nidogen: a new, self-aggregating basement membrane protein . Eur J Biochem. 1983;137:455-465.Crossref 17. Goldsmith LA, Briggaman RA. Monoclonal antibodies to anchoring fibrils for the diagnosis of epidermolysis bullosa . J Invest Dermatol. 1983;81:464-466.Crossref 18. Verrando P, Ortonne J-P, Pautrat G, Hsi B-L, Yeh C-J. Identification of a 37 kilodalton protein at the epidermal basement membrane by anti-serum to human amnion . J Invest Dermatol. 1986;86:190-196.Crossref 19. Fine JD, Gay S. A ubiquitous non-collagenous lamina densa component of basement membrane detected by monoclonal antibody technique . J Invest Dermatol. 1986;86:286-289.Crossref 20. Verrando P, Pisani A, Ortonne J-P. The new basement membrane antigen recognized by the monoclonal antibody GB3 is a large size glycoprotein: modulation of its expression by retinoic acid . Biochim Biophys Acta. 1988;942:45-56.Crossref 21. Verrando P, Hsi B-L, Yeh C-J, Pisano A, Serleys N, Ortonne J-P. Monoclonal antibody GB3: a new probe for the study of human basement membranes and hemidesmosomes . Exp Cell Res. 1987;170:116-128.Crossref 22. Fine JD, Horiguchi Y, Jester J, Couchman J. Detection and partial characterization of a mid-lamina lucida-hemidesmosome-associated antigen (19-DEJ-1) present within human skin . J Invest Dermatol. 1989;92:825-830.Crossref 23. Eady RAJ, Tidman MJ, Heagerty AHM, Kennedy AR. Approaches to the study of epidermolysis bullosa . Curr Probl Dermatol. 1987;17:127-141. 24. Fine JD. Altered skin basement membrane antigenicity in epidermolysis bullosa . In: Wuepper KD, Gedde-Dahl T Jr, eds. Biology of Heritable Skin Diseases . New York, NY: S Karger AG; 1987:111-126. 25. Fine JD. Antigenic features and structural correlates of basement membranes: relationship to epidermolysis bullosa . Arch Dermatol. 1988;124:713-717.Crossref 26. Kennedy AR, Heagerty AHM, Ortonne J-P, Hsi B-L, Yeh C-JG, Eady RAJ. Abnormal binding of an anti-amnion antibody to epidermal basement membrane provides a novel diagnostic probe for junctional epidermolysis bullosa . Br J Dermatol. 1985;113:651-659.Crossref 27. Heagerty AHM, Kennedy AR, Eady RAJ, et al. GB3 monoclonal antibody for diagnosis of junctional epidermolysis bullosa . Lancet. 1986;1:860.Crossref 28. Heagerty AHM, Eady RAJ, Kennedy AR, et al. Rapid prenatal diagnosis of epidermolysis bullosa letalis using GB3 monoclonal antibody . Br J Dermatol. 1987;117:271-275.Crossref 29. Heagerty AHM, Kennedy AR, Gunner DB, Eady RAJ. Rapid prenatal diagnosis and exclusion of epidermolysis bullosa using novel antibody probes . J Invest Dermatol. 1986;86:603-605.Crossref 30. Fine JD. GB3 antigen is an accurate marker of only the Herlitz subset of junctional epidermolysis bullosa . Clin Res. 1990; 38:72A. 31. Fine JD, Horiguchi Y, Couchman JR. 19-DEJ-1, a hemidesmosome-anchoring filament complex-associated monoclonal antibody: definition of a new skin basement membrane antigenic defect in junctional and dystrophic epidermolysis bullosa . Arch Dermatol. 1989;125:520-523.Crossref 32. Hintner H, Stingl G, Schuler G, et al. Immunofluorescence mapping of antigenic determinants within the dermal-epidermal junction in mechanobullous diseases . J Invest Dermatol. 1981; 76:113-118.Crossref 33. Regnier M, Vaigot P, Michel S, Prunieras M. Localization of bullous pemphigoid antigen (BPA) in isolated human keratinocytes . J Invest Dermatol. 1985;85:187-190.Crossref 34. Mutasim DF, Takahashi Y, Labib RS, Anhalt GJ, Patel HP, Diaz LA. A pool of bullous pemphigoid antigen(s) is intracellular and associated with basal cell cytoskeleton-hemidesmosome complex . J Invest Dermatol. 1985;84:47-53.Crossref 35. Westgate GE, Weaver AC, Couchman JR. Bullous pemphigoid antigen localization suggests an intracellur association with hemidesomosomes . J Invest Dermatol. 1985;84:218-224.Crossref 36. Tidman MJ, Eady RAJ. Hemidesmosomes heterogeneity in junctional epidermolysis bullosa revealed by morphometric analysis . J Invest Dermatol. 1986;86:51-56.Crossref 37. Lunstrum GP, Rousselle P, Keene DR, Burgeson RE. Identification of a new basement membrane antigen which localizes to the lamina lucida and displays a novel tissue distribution . J Cell Biol. 1989;109:15A.

Journal

Archives of DermatologyAmerican Medical Association

Published: Sep 1, 1990

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