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Measuring Lymphocytes-Reply

Measuring Lymphocytes-Reply Abstract In Reply.— The strength of our laboratory evaluation of cutaneous T-cell lymphomas lies in the combined approach of using histology, immunohistochemistry, and ultrastructural morphometry.1 McNutt disagrees, however, as to the way the ultrastructural morphometry was performed, specifically, the evaluation of cells based on chromatin patterns. He specifies that blast transformation and abnormal blastic forms can provide important clues to the diagnosis of mycosis fungoides (MF). Blast transformation is, indeed, an important clue in the diagnosis of MF but, in our laboratory, blast transformation is evaluated using immunohistochemistry and histology, not electron microscopy.' The specific immunophenotype of blast cells is crucial to the distinction between MF and peripheral T-cell lymphoma, the latter of which can have a most aggressive course.2In our experience, electron microscopy is most useful in evaluating lymphoid infiltrates that, at the light microscopic level, are morphologically consistent with either early MF or chronic dermatitis (ie, mature References 1. Payne CM, Grogan TM, Lynch PJ: An ultrastructural morphometric and immunohistochemical analysis of cutaneous lymphomas and benign lymphocytic infiltrates of skin: Useful criteria for diagnosis . Arch Dermatol 1986;122:1139-1154.Crossref 2. Grogan TM, Fielder K, Rangel C, et al: Peripheral T-cell lymphoma: Aggressive disease with heterogeneous immunotypes . Am J Clin Pathol 1985;83:279-288. 3. Payne CM, Nagle RB, Lynch PJ: Quantitative electron microscopy in the diagnosis of mycosis fungoides: A simple analysis of lymphocytic nuclear convolutions . Arch Dermatol 1984; 120:63-75.Crossref 4. Grogan TM, Payne CM, Payne TB, et al: Cutaneous myiasis: Immunohistologic and ultrastructural morphometric features of a human botfly lesion . Am J Dermatopathol , in press. 5. Payne CM, Hicks MJ, Kim A: Ultrastructural morphometric analysis of normal human lymphocytes stimulated in vitro with mitogens and antigens . Am J Pathol 1985;120:263-275. 6. Payne CM, Hicks MJ, Bjore CG Jr, et al: Ultrastructural morphometric analysis of nuclear contour irregularity in normal cord and adult blood: Correlation with distinct lymphocyte subpopulations . Diagn Immunol , in press. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Dermatology American Medical Association

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References (6)

Publisher
American Medical Association
Copyright
Copyright © 1987 American Medical Association. All Rights Reserved.
ISSN
0003-987X
eISSN
1538-3652
DOI
10.1001/archderm.1987.01660290029006
Publisher site
See Article on Publisher Site

Abstract

Abstract In Reply.— The strength of our laboratory evaluation of cutaneous T-cell lymphomas lies in the combined approach of using histology, immunohistochemistry, and ultrastructural morphometry.1 McNutt disagrees, however, as to the way the ultrastructural morphometry was performed, specifically, the evaluation of cells based on chromatin patterns. He specifies that blast transformation and abnormal blastic forms can provide important clues to the diagnosis of mycosis fungoides (MF). Blast transformation is, indeed, an important clue in the diagnosis of MF but, in our laboratory, blast transformation is evaluated using immunohistochemistry and histology, not electron microscopy.' The specific immunophenotype of blast cells is crucial to the distinction between MF and peripheral T-cell lymphoma, the latter of which can have a most aggressive course.2In our experience, electron microscopy is most useful in evaluating lymphoid infiltrates that, at the light microscopic level, are morphologically consistent with either early MF or chronic dermatitis (ie, mature References 1. Payne CM, Grogan TM, Lynch PJ: An ultrastructural morphometric and immunohistochemical analysis of cutaneous lymphomas and benign lymphocytic infiltrates of skin: Useful criteria for diagnosis . Arch Dermatol 1986;122:1139-1154.Crossref 2. Grogan TM, Fielder K, Rangel C, et al: Peripheral T-cell lymphoma: Aggressive disease with heterogeneous immunotypes . Am J Clin Pathol 1985;83:279-288. 3. Payne CM, Nagle RB, Lynch PJ: Quantitative electron microscopy in the diagnosis of mycosis fungoides: A simple analysis of lymphocytic nuclear convolutions . Arch Dermatol 1984; 120:63-75.Crossref 4. Grogan TM, Payne CM, Payne TB, et al: Cutaneous myiasis: Immunohistologic and ultrastructural morphometric features of a human botfly lesion . Am J Dermatopathol , in press. 5. Payne CM, Hicks MJ, Kim A: Ultrastructural morphometric analysis of normal human lymphocytes stimulated in vitro with mitogens and antigens . Am J Pathol 1985;120:263-275. 6. Payne CM, Hicks MJ, Bjore CG Jr, et al: Ultrastructural morphometric analysis of nuclear contour irregularity in normal cord and adult blood: Correlation with distinct lymphocyte subpopulations . Diagn Immunol , in press.

Journal

Archives of DermatologyAmerican Medical Association

Published: May 1, 1987

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