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Histochemistry and Fine Structure of Oral Lesions of Mucous Membrane Pemphigoid: Preliminary Observations

Histochemistry and Fine Structure of Oral Lesions of Mucous Membrane Pemphigoid: Preliminary... Abstract Histochemical and fine structural studies of oral vesicular lesions of mucous membrane pemphigoid were performed. Light microscopy revealed that the lesion consisted of a clean separation of epithelium from connective tissue, with epithelial cells remaining intact and basement membrane material adhering to both epithelium and connective tissue. Electron microscopy revealed changes in the tissue adjacent to the developing vesicle, including increased cellularity in the connective tissue, increased amount of basal lamina material, evidence of increased activity of basal cells, projections of basal cells through the basal lamina, and decreased numbers of anchoring fibrils. Sections through vesicles revealed relatively intact basal cells with separation of basal lamina; ie, portions of basal lamina were adherent to both epithelium and connective tissue. The primary site of pathogenesis of oral lesions of mucous membrane pemphigoid is the basement membrane. References 1. Lever WF: Pemphigus . Medicine 32:1-23, 1953.Crossref 2. McCarthy PL, Shklar G: Benign mucous membrane pemphigus . New Eng J Med 258:726-731, 1958.Crossref 3. Pearse EAG: Histochemistry: Theoretical and Applied . Boston, Little Brown & Co, 1960, p 205. 4. Kronman JH, Chauncey HH: Testosterone-induced changes in salivary gland histochemistry in the female golden hamster . J Oral Ther 1:392-405, 1965. 5. Barka T, Anderson PJ: Histochemistry . New York, Harper & Row, 1963, pp 97-102. 6. Lillie RD: Reticulum staining with Schiff reagent after oxidation by acidified sodium periodate . J Lab Clin Med 32:910-911, 1947. 7. McManus JFA: Histological demonstration of mucin after periodic acid . Nature 158:202, 1946.Crossref 8. Glenner GG, Lillie RD: Observations of the diazotization coupling reaction for the histochemical demonstration of tyrosine: Metal chelation and formazon variants . J Histochem Cytochem 7:416-422, 1959.Crossref 9. Barnett RJ, Seligman AM: Histochemical demonstration of protein-bound sulfhydryl groups . Science 116:323, 1952.Crossref 10. Barnett RJ, Seligman AM: Histochemical demonstration of sulfhydryl and disulfide groups of protein . J Nat Cancer Inst 14:769, 1954. 11. Watson ML: Staining of tissue sections for electron microscopy with heavy metals . J Biophys Biochem Cytol 4:475-478, 1958.Crossref 12. Reynolds ES: The use of lead citrate at high pH as an electron opaque stain in electron microscopy . J Cell Biol 17:208-212, 1963.Crossref 13. Shklar G, McCarthy PL: Oral manifestations of benign mucous membrane pemphigus (mucous membrane pemphigoid) . Oral Surg 14:164-181, 1961.Crossref 14. Shklar G, Meyer I, Zacarian S: Oral lesions in bullous pemphigoid . Arch Derm 99:663-670, 1969.Crossref 15. Jordan RE, Beutner EH, Witebsky E, et al: Basement zone antibodies in bullous pemphigoid . JAMA 200:751, 1967.Crossref 16. Susi FR: Anchoring fibrils in the attachment of epithelium to connective tissue in oral mucous membranes . J Dent Res 48:144-148, 1969.Crossref 17. Susi FR, Belt WD, Kelly JW: Fine structure of fibrillar complexes associated with the basement membrane in human oral mucosa . J Cell Biol 34:686-690, 1967.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Dermatology American Medical Association

Histochemistry and Fine Structure of Oral Lesions of Mucous Membrane Pemphigoid: Preliminary Observations

Susi, Frank R.; Shklar, Gerald
Archives of Dermatology , Volume 104 (3) – Sep 1, 1971
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References (18)

Publisher
American Medical Association
Copyright
Copyright © 1971 American Medical Association. All Rights Reserved.
ISSN
0003-987X
eISSN
1538-3652
DOI
10.1001/archderm.1971.04000210018004
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Abstract

Abstract Histochemical and fine structural studies of oral vesicular lesions of mucous membrane pemphigoid were performed. Light microscopy revealed that the lesion consisted of a clean separation of epithelium from connective tissue, with epithelial cells remaining intact and basement membrane material adhering to both epithelium and connective tissue. Electron microscopy revealed changes in the tissue adjacent to the developing vesicle, including increased cellularity in the connective tissue, increased amount of basal lamina material, evidence of increased activity of basal cells, projections of basal cells through the basal lamina, and decreased numbers of anchoring fibrils. Sections through vesicles revealed relatively intact basal cells with separation of basal lamina; ie, portions of basal lamina were adherent to both epithelium and connective tissue. The primary site of pathogenesis of oral lesions of mucous membrane pemphigoid is the basement membrane. References 1. Lever WF: Pemphigus . Medicine 32:1-23, 1953.Crossref 2. McCarthy PL, Shklar G: Benign mucous membrane pemphigus . New Eng J Med 258:726-731, 1958.Crossref 3. Pearse EAG: Histochemistry: Theoretical and Applied . Boston, Little Brown & Co, 1960, p 205. 4. Kronman JH, Chauncey HH: Testosterone-induced changes in salivary gland histochemistry in the female golden hamster . J Oral Ther 1:392-405, 1965. 5. Barka T, Anderson PJ: Histochemistry . New York, Harper & Row, 1963, pp 97-102. 6. Lillie RD: Reticulum staining with Schiff reagent after oxidation by acidified sodium periodate . J Lab Clin Med 32:910-911, 1947. 7. McManus JFA: Histological demonstration of mucin after periodic acid . Nature 158:202, 1946.Crossref 8. Glenner GG, Lillie RD: Observations of the diazotization coupling reaction for the histochemical demonstration of tyrosine: Metal chelation and formazon variants . J Histochem Cytochem 7:416-422, 1959.Crossref 9. Barnett RJ, Seligman AM: Histochemical demonstration of protein-bound sulfhydryl groups . Science 116:323, 1952.Crossref 10. Barnett RJ, Seligman AM: Histochemical demonstration of sulfhydryl and disulfide groups of protein . J Nat Cancer Inst 14:769, 1954. 11. Watson ML: Staining of tissue sections for electron microscopy with heavy metals . J Biophys Biochem Cytol 4:475-478, 1958.Crossref 12. Reynolds ES: The use of lead citrate at high pH as an electron opaque stain in electron microscopy . J Cell Biol 17:208-212, 1963.Crossref 13. Shklar G, McCarthy PL: Oral manifestations of benign mucous membrane pemphigus (mucous membrane pemphigoid) . Oral Surg 14:164-181, 1961.Crossref 14. Shklar G, Meyer I, Zacarian S: Oral lesions in bullous pemphigoid . Arch Derm 99:663-670, 1969.Crossref 15. Jordan RE, Beutner EH, Witebsky E, et al: Basement zone antibodies in bullous pemphigoid . JAMA 200:751, 1967.Crossref 16. Susi FR: Anchoring fibrils in the attachment of epithelium to connective tissue in oral mucous membranes . J Dent Res 48:144-148, 1969.Crossref 17. Susi FR, Belt WD, Kelly JW: Fine structure of fibrillar complexes associated with the basement membrane in human oral mucosa . J Cell Biol 34:686-690, 1967.Crossref

Journal

Archives of Dermatology – American Medical Association

Published: Sep 1, 1971

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