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Exfoliative Cytology of Human Horny Layer: Methods of Cell Removal and Microscopic Techniques

Exfoliative Cytology of Human Horny Layer: Methods of Cell Removal and Microscopic Techniques Abstract A procedure for studying the cytology and morphology of the horny cells of the skin has been described. It is based upon removal of horny cells directly onto adhesive coated glass slides. By viewing the cells in their broad axes, many details may be seen which are concealed in traditional transverse sections. Optical systems and staining procedures are described to facilitate examination of normal and abnormal horny cells. References 1. Kligman, A.M.: " The Biology of the Stratum Corneum ," in Montagna, W., and Lobitz, W.C. (eds.): The Epidermis , New York: Academic Press, 1964. 2. Wolf, J.: Die innere Struktur der Zellen des Stratum desquamans der menschlichen Epidermis , Z Mikr Anat Forsch 46:170-202, 1939. 3. Wolf, J.: Das Oberflaechenrelief der menschlichen Haut , Z Mikr Anat Forsch 47:351-400, 1940. 4. Pinkus, H.: Examination of the Epidermis by the Strip Method of Removing Horny Layers , J Invest Derm 16:383, 1951 5. 19:431, 1952. 6. Keddie, F., and Sandi, D.: Morphology of the Horny Cells of the Superficial Stratum Corneum , J Invest Derm 44:135, 1965. 7. Facq, J.; Kirk, D.L.; and Rebell, G.: A Simple Replica Technique For Observation of Human Skin , J Soc Cosm Chem 15:87, 1964. 8. Brody, I.: The Ultrastructure of the Tonofilaments in the Keratinization Process of Normal Human Epidermis , J Ultrastruc Res 2:482, 1959.Crossref 9. Matoltsy, A.G., and Matoltsy, M.N.: The Membrane Protein of Horny Cells , J Invest Derm 46:27, 1966. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Dermatology American Medical Association

Exfoliative Cytology of Human Horny Layer: Methods of Cell Removal and Microscopic Techniques

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References (11)

Publisher
American Medical Association
Copyright
Copyright © 1967 American Medical Association. All Rights Reserved.
ISSN
0003-987X
eISSN
1538-3652
DOI
10.1001/archderm.1967.01610050094016
Publisher site
See Article on Publisher Site

Abstract

Abstract A procedure for studying the cytology and morphology of the horny cells of the skin has been described. It is based upon removal of horny cells directly onto adhesive coated glass slides. By viewing the cells in their broad axes, many details may be seen which are concealed in traditional transverse sections. Optical systems and staining procedures are described to facilitate examination of normal and abnormal horny cells. References 1. Kligman, A.M.: " The Biology of the Stratum Corneum ," in Montagna, W., and Lobitz, W.C. (eds.): The Epidermis , New York: Academic Press, 1964. 2. Wolf, J.: Die innere Struktur der Zellen des Stratum desquamans der menschlichen Epidermis , Z Mikr Anat Forsch 46:170-202, 1939. 3. Wolf, J.: Das Oberflaechenrelief der menschlichen Haut , Z Mikr Anat Forsch 47:351-400, 1940. 4. Pinkus, H.: Examination of the Epidermis by the Strip Method of Removing Horny Layers , J Invest Derm 16:383, 1951 5. 19:431, 1952. 6. Keddie, F., and Sandi, D.: Morphology of the Horny Cells of the Superficial Stratum Corneum , J Invest Derm 44:135, 1965. 7. Facq, J.; Kirk, D.L.; and Rebell, G.: A Simple Replica Technique For Observation of Human Skin , J Soc Cosm Chem 15:87, 1964. 8. Brody, I.: The Ultrastructure of the Tonofilaments in the Keratinization Process of Normal Human Epidermis , J Ultrastruc Res 2:482, 1959.Crossref 9. Matoltsy, A.G., and Matoltsy, M.N.: The Membrane Protein of Horny Cells , J Invest Derm 46:27, 1966.

Journal

Archives of DermatologyAmerican Medical Association

Published: Nov 1, 1967

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