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Preparation of Permanent Stained Slide Cultures

Preparation of Permanent Stained Slide Cultures Abstract THE USE OF the slide culture technique is well known among dermatologists as a valuable diagnostic and teaching aid in mycology. One of the disadvantages of this procedure has been the inability to maintain permanent stained mounts for future reference in a teaching program or when attempting to identify an unknown fungus. It is the purpose of this paper to describe a simple method of preparing permanent stained slide cultures. Materials and Methods The material needed is that used for the standard slide culture technique consisting of a Petri dish, glass tubing, microscope slide, cover glass, and agar.1 The glass tubing is placed in the Petri dish and the glass slide is placed on it. The entire unit is then autoclaved. If an autoclave is not available, flaming will be satisfactory. A drop of melted agar is then pipetted on one end of the sterilized References 1. Ferguson, J., and Prazak, G.: Slide Culture , AMA Arch Derm 81:974, 1960.Crossref 2. Gregersen, A.: Dermatological Histopathologic Technic , Los Angeles: University of Southern California Press, 1956, p 49. 3. Kaden, R.: Modified Agar Block Method for Study of Fungi , Derm Wschr 144:911, 1961. 4. Knaysi, G.: New Technique of Growing Molds in Slide Culture Under Environmental Conditions Similar to Those Prevailing in Petri Dish , J Bact 73:431, 1957. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Dermatology American Medical Association

Preparation of Permanent Stained Slide Cultures

CURTIS, JOHN W.
Archives of Dermatology , Volume 91 (3) – Mar 1, 1965
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References (4)

Publisher
American Medical Association
Copyright
Copyright © 1965 American Medical Association. All Rights Reserved.
ISSN
0003-987X
eISSN
1538-3652
DOI
10.1001/archderm.1965.01600090088015
Publisher site
See Article on Publisher Site

Abstract

Abstract THE USE OF the slide culture technique is well known among dermatologists as a valuable diagnostic and teaching aid in mycology. One of the disadvantages of this procedure has been the inability to maintain permanent stained mounts for future reference in a teaching program or when attempting to identify an unknown fungus. It is the purpose of this paper to describe a simple method of preparing permanent stained slide cultures. Materials and Methods The material needed is that used for the standard slide culture technique consisting of a Petri dish, glass tubing, microscope slide, cover glass, and agar.1 The glass tubing is placed in the Petri dish and the glass slide is placed on it. The entire unit is then autoclaved. If an autoclave is not available, flaming will be satisfactory. A drop of melted agar is then pipetted on one end of the sterilized References 1. Ferguson, J., and Prazak, G.: Slide Culture , AMA Arch Derm 81:974, 1960.Crossref 2. Gregersen, A.: Dermatological Histopathologic Technic , Los Angeles: University of Southern California Press, 1956, p 49. 3. Kaden, R.: Modified Agar Block Method for Study of Fungi , Derm Wschr 144:911, 1961. 4. Knaysi, G.: New Technique of Growing Molds in Slide Culture Under Environmental Conditions Similar to Those Prevailing in Petri Dish , J Bact 73:431, 1957.

Journal

Archives of Dermatology – American Medical Association

Published: Mar 1, 1965

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