Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

pH Signature of Skin Carcinoma: An Application of the pH Signature Technique to Sections of Skin of Basal- and Squamous-Cell Carcinoma

pH Signature of Skin Carcinoma: An Application of the pH Signature Technique to Sections of Skin... Abstract Comparisons of microscopic pattern of neoplastic and non-neoplastic cells are of daily practical importance in the diagnosis of tumors and the management of cancer patients. Comparisons of chemical pattern are still largely in the field of research1 but are an essential step in furthering our understanding of neoplasia.2 For some years we3 have been applying new cell techniques developed by histochemists and cytophysicists to the study of human cancer. One technique of considerable potential is the "pH signature."4,5 Proteins differ according to the nature and number of their constituent amino acids and conjugated substances.6 Proteins are amphoteric. They contain at the same time both basic and acidic groups, which, by their dissociation, give rise to positive and negative charges on the protein molecule. The ability of proteins to take up acid or basic dye according to the pH of the solution is an expression of References 1. Foraker, A. G., and Wingo, W. J.: Succinic Dehydrogenase Activity, Protein Bound Sulfhydryl and Disulfide Groups in Squamous Cell Carcinoma of the Skin , Surg. Gynec. & Obst. 101:346-352, 1955. 2. Foraker, A. G.: A Histochemical Study of Breast Carcinoma , Surg. Gynec. & Obst. 102:1-8, 1956. 3. Foraker, A. G., and Denham, S. W.: Squamous Cell Carcinoma of the Uterine Cervix: A Histochemical Review , Am. J. Obst. & Gynec. 74: 13-24, 1959. 4. Dempsey, E. W., and Singer, M.: Observations on the Chemical Cytology of the Thyroid Gland at Different Functional Stages , Endocrinology 38:270-295, 1946. 5. Singer, M., and Wislocki, G. B.: Affinity of Syncytium, Fibrin and Fibrinoid of Human Placenta for Acid and Basic Dyes Under Controlled Conditions of Staining , Anat. Rec. 102: 175-193, 1948. 6. Singer, M.: Factors Which Control the Staining of Tissue Sections with Acid and Basic Dyes , Int. Rev. Cytol. 1:211-255, 1952. 7. Barrnett, R. J.: Personal communication to the author. 8. Barrnett, R. J.: Histochemical Demonstration of Proteins , in Histochemistry (Manual of Post-graduate Medical Study) , (June 10) -22, 1957, University of Kansas School of Medicine, Kansas City, Kan. 9. De Robertis, E. D. P.; Nowinski, W. W., and Saez, F. A.: General Cytology , Ed. 2, Philadelphia, W. B. Saunders Company, 1954. 10. Kurnick, N. B.: Histochemistry of Nucleic Acids , Int. Rev. Cytol. 4:221-268, 1955. 11. Leuchtenberger, C.; Leuchtenberger, R., and Davis, A. M.: A Microspectrophotometric Study of the Desoxyribose Nucleic Acid (DNA) Content in Cells of Normal and Malignant Human Tissues , Am. J. Path. 30:65-85, 1954. 12. Singer, M.: Personal communication to the author. 13. Wheatley, R. E., and Foraker, A. G.: "pH Signature" Studies in Squamous Mucosa of the Uterine Cervix: Normal Exocervical, Metaplastic, Dysplastic, Intraepithelial and Invasive Carcinomatous , Am. J. Obst. & Gynec. 80:1074-1082, 1960. 14. Foraker, A. G.: "pH Signature" of Adenocarcinoma of Colon in Man , J. Histochem. 8: 284-289, 1960. 15. Brachet, J.: Biochemical Cytology , New York, Academic Press, Inc., 1957, p. 179. 16. Leuchtenberger, C.: Quantitative Determination of DNA in Cells by Feulgen Microspectrophotometry , in General Cytochemical Methods , edited by G. H. Bourne and J. F. Danielli, New York, Academic Press, Inc., 1957, Vol. 1, p. 220-278. 17. Rothman, S.: Physiology and Biochemistry of the Skin , Chicago, The University of Chicago Press, 1954. 18. Foraker, A. G., and Wingo, W. J.: Histochemical Studies of Skin , A.M.A. Arch. Dermat. 17:1-6, 1955. 19. Foraker, A. G., and Wingo, W. J.: Protein Bound Sulfhydryl and Disulfide Groups and Succinic Dehydrogenase Activity in Basal Cell Carcinoma of the Skin , Exper. Med. & Surg. 14: 122-129, 1956. 20. Foraker, A. G., and Wingo, W. J.: Senile and Seborrheic Keratoses: Localization of Succinic Dehydrogenase, Protein Bound Sulfhydryl and Disulfide Groups , Am. J. Path. 32:521-533, 1956. 21. Foraker, A. G.: Histochemical Studies in Squamous Carcinoma , Cancer 9:367-373, 1956. 22. Steiner, K.: A Histochemical Study of Hyperkeratoses , A.M.A. Arch. Dermat. 79:436-443, 1959. 23. Foraker, A. G., and Marino, G.: Estudios bioquimicos en el condrosarcoma , Folia Clín. Internac. 6:56-60, 1956. 24. Foraker, A. G.: Analysis of Hyperchromatism in the Histologic Diagnosis of Carcinoma of the Cervix Uteri , A.M.A. Arch. Path. 53:250-256, 1952. 25. Foraker, A. G., and Reagan, J. W.: Nuclear Size and Nuclear: Cytoplasmic Ratio in the Delineation of Atypical Hyperplasia of the Uterine Cervix , Cancer 9:470-479, 1956. 26. Foraker, A. G., and Reagan, J. W.: Nuclear Mass and Allied Phenomena in Normal Exocervical Mucosa, Squamous Metaplasia, Atypical Hyperplasia, Intraepithelial Carcinoma and Invasive Squamous Cell Carcinoma of the Uterine Cervix , Cancer 12:894-905, 1959. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Dermatology American Medical Association

pH Signature of Skin Carcinoma: An Application of the pH Signature Technique to Sections of Skin of Basal- and Squamous-Cell Carcinoma

Archives of Dermatology , Volume 83 (3) – Mar 1, 1961

Loading next page...
 
/lp/american-medical-association/ph-signature-of-skin-carcinoma-an-application-of-the-ph-signature-7wU5KHVzPT

References (27)

Publisher
American Medical Association
Copyright
Copyright © 1961 American Medical Association. All Rights Reserved.
ISSN
0003-987X
eISSN
1538-3652
DOI
10.1001/archderm.1961.01580090042004
Publisher site
See Article on Publisher Site

Abstract

Abstract Comparisons of microscopic pattern of neoplastic and non-neoplastic cells are of daily practical importance in the diagnosis of tumors and the management of cancer patients. Comparisons of chemical pattern are still largely in the field of research1 but are an essential step in furthering our understanding of neoplasia.2 For some years we3 have been applying new cell techniques developed by histochemists and cytophysicists to the study of human cancer. One technique of considerable potential is the "pH signature."4,5 Proteins differ according to the nature and number of their constituent amino acids and conjugated substances.6 Proteins are amphoteric. They contain at the same time both basic and acidic groups, which, by their dissociation, give rise to positive and negative charges on the protein molecule. The ability of proteins to take up acid or basic dye according to the pH of the solution is an expression of References 1. Foraker, A. G., and Wingo, W. J.: Succinic Dehydrogenase Activity, Protein Bound Sulfhydryl and Disulfide Groups in Squamous Cell Carcinoma of the Skin , Surg. Gynec. & Obst. 101:346-352, 1955. 2. Foraker, A. G.: A Histochemical Study of Breast Carcinoma , Surg. Gynec. & Obst. 102:1-8, 1956. 3. Foraker, A. G., and Denham, S. W.: Squamous Cell Carcinoma of the Uterine Cervix: A Histochemical Review , Am. J. Obst. & Gynec. 74: 13-24, 1959. 4. Dempsey, E. W., and Singer, M.: Observations on the Chemical Cytology of the Thyroid Gland at Different Functional Stages , Endocrinology 38:270-295, 1946. 5. Singer, M., and Wislocki, G. B.: Affinity of Syncytium, Fibrin and Fibrinoid of Human Placenta for Acid and Basic Dyes Under Controlled Conditions of Staining , Anat. Rec. 102: 175-193, 1948. 6. Singer, M.: Factors Which Control the Staining of Tissue Sections with Acid and Basic Dyes , Int. Rev. Cytol. 1:211-255, 1952. 7. Barrnett, R. J.: Personal communication to the author. 8. Barrnett, R. J.: Histochemical Demonstration of Proteins , in Histochemistry (Manual of Post-graduate Medical Study) , (June 10) -22, 1957, University of Kansas School of Medicine, Kansas City, Kan. 9. De Robertis, E. D. P.; Nowinski, W. W., and Saez, F. A.: General Cytology , Ed. 2, Philadelphia, W. B. Saunders Company, 1954. 10. Kurnick, N. B.: Histochemistry of Nucleic Acids , Int. Rev. Cytol. 4:221-268, 1955. 11. Leuchtenberger, C.; Leuchtenberger, R., and Davis, A. M.: A Microspectrophotometric Study of the Desoxyribose Nucleic Acid (DNA) Content in Cells of Normal and Malignant Human Tissues , Am. J. Path. 30:65-85, 1954. 12. Singer, M.: Personal communication to the author. 13. Wheatley, R. E., and Foraker, A. G.: "pH Signature" Studies in Squamous Mucosa of the Uterine Cervix: Normal Exocervical, Metaplastic, Dysplastic, Intraepithelial and Invasive Carcinomatous , Am. J. Obst. & Gynec. 80:1074-1082, 1960. 14. Foraker, A. G.: "pH Signature" of Adenocarcinoma of Colon in Man , J. Histochem. 8: 284-289, 1960. 15. Brachet, J.: Biochemical Cytology , New York, Academic Press, Inc., 1957, p. 179. 16. Leuchtenberger, C.: Quantitative Determination of DNA in Cells by Feulgen Microspectrophotometry , in General Cytochemical Methods , edited by G. H. Bourne and J. F. Danielli, New York, Academic Press, Inc., 1957, Vol. 1, p. 220-278. 17. Rothman, S.: Physiology and Biochemistry of the Skin , Chicago, The University of Chicago Press, 1954. 18. Foraker, A. G., and Wingo, W. J.: Histochemical Studies of Skin , A.M.A. Arch. Dermat. 17:1-6, 1955. 19. Foraker, A. G., and Wingo, W. J.: Protein Bound Sulfhydryl and Disulfide Groups and Succinic Dehydrogenase Activity in Basal Cell Carcinoma of the Skin , Exper. Med. & Surg. 14: 122-129, 1956. 20. Foraker, A. G., and Wingo, W. J.: Senile and Seborrheic Keratoses: Localization of Succinic Dehydrogenase, Protein Bound Sulfhydryl and Disulfide Groups , Am. J. Path. 32:521-533, 1956. 21. Foraker, A. G.: Histochemical Studies in Squamous Carcinoma , Cancer 9:367-373, 1956. 22. Steiner, K.: A Histochemical Study of Hyperkeratoses , A.M.A. Arch. Dermat. 79:436-443, 1959. 23. Foraker, A. G., and Marino, G.: Estudios bioquimicos en el condrosarcoma , Folia Clín. Internac. 6:56-60, 1956. 24. Foraker, A. G.: Analysis of Hyperchromatism in the Histologic Diagnosis of Carcinoma of the Cervix Uteri , A.M.A. Arch. Path. 53:250-256, 1952. 25. Foraker, A. G., and Reagan, J. W.: Nuclear Size and Nuclear: Cytoplasmic Ratio in the Delineation of Atypical Hyperplasia of the Uterine Cervix , Cancer 9:470-479, 1956. 26. Foraker, A. G., and Reagan, J. W.: Nuclear Mass and Allied Phenomena in Normal Exocervical Mucosa, Squamous Metaplasia, Atypical Hyperplasia, Intraepithelial Carcinoma and Invasive Squamous Cell Carcinoma of the Uterine Cervix , Cancer 12:894-905, 1959.

Journal

Archives of DermatologyAmerican Medical Association

Published: Mar 1, 1961

There are no references for this article.