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USE OF A CYCLOHEXIMIDE MEDIUM FOR ISOLATION OF DERMATOPHYTES FROM CLINICAL MATERIALS

USE OF A CYCLOHEXIMIDE MEDIUM FOR ISOLATION OF DERMATOPHYTES FROM CLINICAL MATERIALS Abstract THE ISOLATION of dermatophytes from clinical materials frequently is complicated by contamination of culture tubes with bacteria and saprophytic fungi. Such specimens as human toenails and the hairs of domestic animals offer a special problem, owing to the presence of large numbers of these contaminants. Bacterial contaminants are generally controllable by the use either of an acid medium, such as Sabouraud dextrose agar at pH 5.5, or of antibacterial antibiotics, or both. Sabouraud dextrose agar at pH 5.5 fortified with penicillin (20 units per milliliter) and streptomycin (40 units per milliliter) has been used routinely at the Mycology Laboratory of the Communicable Disease Center for the isolation of dermatophytes from clinical materials. The frequent occurrence of saprophytic fungi, however, necessitates inoculation of a large number of tubes for each specimen, and repeated subcultures often are needed to separate a suspected dermatophyte colony from growths of the more rapidly developing saprophytes. References 1. Leach, B. E.; Ford, J. H., and Whiffen, A. J.: Actidione, an Antibiotic from Streptomyces Griseus , J. Am. Chem. Soc. 69:474, 1947.Crossref 2. Whiffen, A. J.: The Production, Assay, and Antibiotic Activity of Actidione, an Antibiotic from Streptomyces Griseus , J. Bact. 56:283-291, 1948. 3. Phillips, G. B., and Hanel, E.: Control of Mold Contaminants on Solid Media by the Use of Actidione , J. Bact. 60:104-105, 1950. 4. Carmona de la Fuentes, A.; Trespalacios, F.; Fernández Baquero, G., and Aboulafia, R.: Effect of Actidione on Mold Contaminants and on Human Pathogens , Mycologia 44:170-175, 1952. 5. Georg, L. K.; Ajello, L., and Gordon, M. A.: A Selective Medium for the Isolation of Coccidioides Immitis , Science 114:387-389, 1951.Crossref 6. Trichophyton verrucosum has recently been validated by Ainsworth and Georg (Nomenclature of the Faviform Trichophytons, Mycologia, to be published) as the correct name for the dermatophyte more commonly known to both mycologists and dermatologists under the following species designations: faviforme, album, discoides, and ochraceum. 7. Tanissa, A.: Tinea Tonsurans and Its Mixed Infection , Gaz. méd. port. 3:385-392, 1950. 8. Wooldridge, W. E.: An Unusual Case of Multiple Fungus Infection , A. M. A. Arch Dermat. & Syph. 63:633-635, 1951. 9. Fowle, L. P., and Georg, L. K.: Suppurative Ringworm Contracted from Cattle , Arch. Dermat. & Syph. 56:780-793, 1947. 10. Georg, L. K.: The Relation of Nutrition to the Growth and Morphology of Trichophyton Faviforme , Mycologia 42:683-692, 1950. 11. Littman, M. L.: Growth of Pathogenic Fungi on a New Culture Medium , Am. J. Clin. Path. 18:409-420, 1948. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png A.M.A. Archives of Dermatology & Syphilology American Medical Association

USE OF A CYCLOHEXIMIDE MEDIUM FOR ISOLATION OF DERMATOPHYTES FROM CLINICAL MATERIALS

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References (12)

Publisher
American Medical Association
Copyright
Copyright © 1953 American Medical Association. All Rights Reserved.
ISSN
0096-5979
DOI
10.1001/archderm.1953.01540040013003
Publisher site
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Abstract

Abstract THE ISOLATION of dermatophytes from clinical materials frequently is complicated by contamination of culture tubes with bacteria and saprophytic fungi. Such specimens as human toenails and the hairs of domestic animals offer a special problem, owing to the presence of large numbers of these contaminants. Bacterial contaminants are generally controllable by the use either of an acid medium, such as Sabouraud dextrose agar at pH 5.5, or of antibacterial antibiotics, or both. Sabouraud dextrose agar at pH 5.5 fortified with penicillin (20 units per milliliter) and streptomycin (40 units per milliliter) has been used routinely at the Mycology Laboratory of the Communicable Disease Center for the isolation of dermatophytes from clinical materials. The frequent occurrence of saprophytic fungi, however, necessitates inoculation of a large number of tubes for each specimen, and repeated subcultures often are needed to separate a suspected dermatophyte colony from growths of the more rapidly developing saprophytes. References 1. Leach, B. E.; Ford, J. H., and Whiffen, A. J.: Actidione, an Antibiotic from Streptomyces Griseus , J. Am. Chem. Soc. 69:474, 1947.Crossref 2. Whiffen, A. J.: The Production, Assay, and Antibiotic Activity of Actidione, an Antibiotic from Streptomyces Griseus , J. Bact. 56:283-291, 1948. 3. Phillips, G. B., and Hanel, E.: Control of Mold Contaminants on Solid Media by the Use of Actidione , J. Bact. 60:104-105, 1950. 4. Carmona de la Fuentes, A.; Trespalacios, F.; Fernández Baquero, G., and Aboulafia, R.: Effect of Actidione on Mold Contaminants and on Human Pathogens , Mycologia 44:170-175, 1952. 5. Georg, L. K.; Ajello, L., and Gordon, M. A.: A Selective Medium for the Isolation of Coccidioides Immitis , Science 114:387-389, 1951.Crossref 6. Trichophyton verrucosum has recently been validated by Ainsworth and Georg (Nomenclature of the Faviform Trichophytons, Mycologia, to be published) as the correct name for the dermatophyte more commonly known to both mycologists and dermatologists under the following species designations: faviforme, album, discoides, and ochraceum. 7. Tanissa, A.: Tinea Tonsurans and Its Mixed Infection , Gaz. méd. port. 3:385-392, 1950. 8. Wooldridge, W. E.: An Unusual Case of Multiple Fungus Infection , A. M. A. Arch Dermat. & Syph. 63:633-635, 1951. 9. Fowle, L. P., and Georg, L. K.: Suppurative Ringworm Contracted from Cattle , Arch. Dermat. & Syph. 56:780-793, 1947. 10. Georg, L. K.: The Relation of Nutrition to the Growth and Morphology of Trichophyton Faviforme , Mycologia 42:683-692, 1950. 11. Littman, M. L.: Growth of Pathogenic Fungi on a New Culture Medium , Am. J. Clin. Path. 18:409-420, 1948.

Journal

A.M.A. Archives of Dermatology & Syphilology – American Medical Association

Published: Apr 1, 1953

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