Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

SODIUM AND POTASSIUM CONTENT OF HUMAN EPIDERMIS

SODIUM AND POTASSIUM CONTENT OF HUMAN EPIDERMIS Abstract KNOWLEDGE of the basic chemical composition of epidermal tissue is fundamental to an understanding of many of the problems of dermatology. Preliminary observations of the chemical composition of human epidermis have been made possible by the introduction of a method for the clean, complete separation of epidermis and dermis1 and the development of the flame photometer for measuring small quantities of sodium and potassium in biologic solutions.2 The flame photometer utilizing an internal lithium standard was constructed by one of us (C. L. F.) in accordance with the design of Berry, Chappell and Barnes.3 This instrument has been found, after extensive testing,4 to give accurate and reproducible readings and represents a considerable improvement over the original direct-reading photometer designed by Barnes and associates2 and tested by Hald.5 METHODS AND MATERIAL The material used in this investigation was human skin obtained from amputated parts, with radical mastectomies constituting the main source. References 1. Baumberger, J. P.; Suntzeff, V., and Cowdry, E. V.: Methods for the Separation of Epidermis and Dermis and Some Physiologic and Chemical Properties of Isolated Epidermis , J. Nat. Cancer Inst. 2:413 ( (April) ) 1942. 2. Barnes, R. B.; Richardson, D.; Berry, J. W., and Hood, R. L.: Flame Photometry: A Rapid Analytical Procedure , Indust. & Engin. Chem. (Analyt. Ed.) 17:605 ( (Oct.) ) 1945. 3. Berry, J. W.; Chappell, D. G., and Barnes, R. B.: Improved Method of Flame Photometry , Indust. & Engin. Chem. (Analyt. Ed.) 18:19 ( (Jan.) ) 1946. 4. Fox, C. L., Jr., and Baer, H.: Redistribution of Potassium, Sodium, and Water in Burns and Trauma, and Its Relation to the Phenomena of Shock , Am. J. Physiol. 151:155 ( (Nov.) ) 1947. 5. Hald, P. M.: The Flame Photometer for the Measurement of Sodium and Potassium in Biological Materials , J. Biol. Chem. 167:499 ( (Feb.) ) 1947. 6. Lowry, O. H., and Hastings, A. B.: Histochemical Changes Associated with Aging , J. Biol. Chem. 143:257 ( (March) ) 1942. 7. Zheutlin, H. E. C.: Unpublished data. 8. Eisele, C. W., and Eichelberger, L.: Water, Electrolyte and Nitrogen Content of Human Skin , Proc. Soc. Exper. Biol. & Med. 58:97 ( (Jan.) ) 1945. 9. Suntzeff, V., and Carruthers, C.: The Mineral Composition of Human Epidermis , J. Biol. Chem. 160:567 ( (Oct.) ) 1945. 10. Eichelberger, L.: Eisele, C. W., and Wertzler, D.: The Distribution of Water, Nitrogen, and Electrolytes in Skin , J. Biol. Chem. 151:177 ( (Nov.) ) 1943. 11. Hastings, A. B., and Eichelberger, L.: The Exchange of Salt and Water Between Muscle and Blood , J. Biol. Chem. 117:73 ( (Jan.) ) 1937. 12. Darrow, D. C.: Disturbances in Electrolyte Metabolism in Man and Their Management , Bull. New York Acad. Med. 24:147 ( (March) ) 1948. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Dermatology and Syphilology American Medical Association

SODIUM AND POTASSIUM CONTENT OF HUMAN EPIDERMIS

Loading next page...
 
/lp/american-medical-association/sodium-and-potassium-content-of-human-epidermis-OPI8JkMSwF

References (13)

Publisher
American Medical Association
Copyright
Copyright © 1950 American Medical Association. All Rights Reserved.
ISSN
0096-6029
DOI
10.1001/archderm.1950.01530100041004
Publisher site
See Article on Publisher Site

Abstract

Abstract KNOWLEDGE of the basic chemical composition of epidermal tissue is fundamental to an understanding of many of the problems of dermatology. Preliminary observations of the chemical composition of human epidermis have been made possible by the introduction of a method for the clean, complete separation of epidermis and dermis1 and the development of the flame photometer for measuring small quantities of sodium and potassium in biologic solutions.2 The flame photometer utilizing an internal lithium standard was constructed by one of us (C. L. F.) in accordance with the design of Berry, Chappell and Barnes.3 This instrument has been found, after extensive testing,4 to give accurate and reproducible readings and represents a considerable improvement over the original direct-reading photometer designed by Barnes and associates2 and tested by Hald.5 METHODS AND MATERIAL The material used in this investigation was human skin obtained from amputated parts, with radical mastectomies constituting the main source. References 1. Baumberger, J. P.; Suntzeff, V., and Cowdry, E. V.: Methods for the Separation of Epidermis and Dermis and Some Physiologic and Chemical Properties of Isolated Epidermis , J. Nat. Cancer Inst. 2:413 ( (April) ) 1942. 2. Barnes, R. B.; Richardson, D.; Berry, J. W., and Hood, R. L.: Flame Photometry: A Rapid Analytical Procedure , Indust. & Engin. Chem. (Analyt. Ed.) 17:605 ( (Oct.) ) 1945. 3. Berry, J. W.; Chappell, D. G., and Barnes, R. B.: Improved Method of Flame Photometry , Indust. & Engin. Chem. (Analyt. Ed.) 18:19 ( (Jan.) ) 1946. 4. Fox, C. L., Jr., and Baer, H.: Redistribution of Potassium, Sodium, and Water in Burns and Trauma, and Its Relation to the Phenomena of Shock , Am. J. Physiol. 151:155 ( (Nov.) ) 1947. 5. Hald, P. M.: The Flame Photometer for the Measurement of Sodium and Potassium in Biological Materials , J. Biol. Chem. 167:499 ( (Feb.) ) 1947. 6. Lowry, O. H., and Hastings, A. B.: Histochemical Changes Associated with Aging , J. Biol. Chem. 143:257 ( (March) ) 1942. 7. Zheutlin, H. E. C.: Unpublished data. 8. Eisele, C. W., and Eichelberger, L.: Water, Electrolyte and Nitrogen Content of Human Skin , Proc. Soc. Exper. Biol. & Med. 58:97 ( (Jan.) ) 1945. 9. Suntzeff, V., and Carruthers, C.: The Mineral Composition of Human Epidermis , J. Biol. Chem. 160:567 ( (Oct.) ) 1945. 10. Eichelberger, L.: Eisele, C. W., and Wertzler, D.: The Distribution of Water, Nitrogen, and Electrolytes in Skin , J. Biol. Chem. 151:177 ( (Nov.) ) 1943. 11. Hastings, A. B., and Eichelberger, L.: The Exchange of Salt and Water Between Muscle and Blood , J. Biol. Chem. 117:73 ( (Jan.) ) 1937. 12. Darrow, D. C.: Disturbances in Electrolyte Metabolism in Man and Their Management , Bull. New York Acad. Med. 24:147 ( (March) ) 1948.

Journal

Archives of Dermatology and SyphilologyAmerican Medical Association

Published: Mar 1, 1950

There are no references for this article.