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The galactose-regulated ( GAL ) genes of yeast Saccharomyces cerevisiae are rapidly and efficiently expressed when cells are switched to a medium containing galactose as a sole carbon source. This GAL induction response is tightly controlled by interplay of three regulatory proteins:...
Three mushroom ( Agaricus bisporus ) crops (Crops 1, 2, 3) were grown to evaluate the effects of re-supplementing “spent” mushroom compost (MC) with the crystalline amino acids isoleucine (ile), leucine (leu), valine (val) and phenylalanine (phe) singly or in combination with Fermenten ® or...
Food can harbour harmful pathogenic microorganisms, such as Salmonella spp. that are responsible by serious human illnesses. The use of natural antimicrobial substances can be practical to maintain the fresh image of food, to increase its shelf life, safety and marketability. We therefore...
Secondary metabolites, particularly bioactive compounds, from probiotic bacteria, are good candidates for replacing antibiotics to which bacteria have become resistant. In order to compare bioactive crude material from strain SA14 of Brevibacillus laterosporus with two antibiotics, the MICs of...
Two strains of Bacillus , one from a culture collection ( B. subtilis ATCC 6633) and a wild type ( Bacillus sp. UFLA 817CF) isolated during coffee fermentation in the south of Minas Gerais, Brazil, were evaluated in relation to secretion of alkaline proteases. The strains were grown on nutrient...
The investigation aimed to examine the Streptomyces flora of hydrocarbon-contaminated soil and study their capability to grow on diesel fuel as a sole carbon source and their analysis for the presence of the alkane hydroxylase gene ( alkB ) by PCR. A total of 16 Streptomyces isolates were...
Rhodococcus sp. R 14-2 , isolated from Chinese Jin-hua ham, produces a novel extracellular cholesterol oxidase (COX). The enzyme was extracted from fermentation broth and purified 53.1-fold based on specific activity. The purified enzyme shows a single polypeptide band on SDS-PAGE with an...
High ethanol tolerance is a desired property of industrial yeast strains for efficient ethanol fermentation. In this study, the impact of medium composition on ethanol tolerance of the self-flocculating yeast SPSC01 was investigated using a chemically defined medium. Single-factor experiments...
We investigated the diversity of epibiotic bacteria possessing antimicrobial activity isolated from nine species of red algae, and identified their phylogenetic position. For the isolation of epibiotic bacteria, nine species of red algae, Pachymeniopsis lauceolata , Plocamium telfairiae ,...
The advent of molecular biological techniques and a developing environmental awareness initiated a renewed scientific interest in Polyhydroxyalkanoates (PHAs) and the biosynthetic machinery for PHA metabolism has been the area of research over the last two decades. PHAs are polyesters of...
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