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The ratio k cat / K M – often referred to as the ‘specificity constant’ – is a useful index for comparing the relative rates of an enzyme acting on alternative, competing substrates. However, an alternative description, ‘catalytic efficiency’, is frequently used, and on occasions...
Membranes are essential to a range of applications, including the production of potable water, energy generation, tissue repair, pharmaceutical production, food packaging, and the separations needed for the manufacture of chemicals, electronics and a range of other products. Therefore, they are...
Protein aggregation is a challenge to the successful manufacture of protein therapeutics; it can impose severe limitations on purification yields and compromise formulation stability. Advances in computer power, and the wealth of computational studies pertaining to protein folding, have...
The topic of this review is an alternative technique for somatic cell nuclear transfer. Removal of the zona pellucida facilitates manipulations of mammalian oocytes and early embryos, and problems related to their subsequent culture are commonly overestimated. This approach enables radical...
α-Dystroglycan is a highly glycosylated peripheral protein forming a complex with the membrane-spanning β-dystroglycan and establishing a connection between the extracellular matrix and the cytoskeleton. In skeletal muscle, as part of the larger dystrophin–glycoprotein complex, dystroglycan...
Using RNA interference (RNAi) technology, the levels of a toxic phytoprotectant have recently been reduced specifically in the seeds of cotton to generate a novel dual-purpose crop. By engineering an endogenous terpene pathway, there is now the exciting potential for an added-value, genetically...
Avidin and streptavidin are widely used in (strept)avidin–biotin technology, which is based on their tight biotin-binding capability. These techniques are exceptionally diverse, ranging from simple purification and labeling methods to sophisticated drug pre-targeting and nanostructure-building...
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