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Little is known about phototactic signal transduction in flagellate green algae; therefore, eyespot apparatuses, which are the light-sensitive “organelles” involved in photoorientation of these algae, were isolated and analyzed for the presence of heterotrimeric guanine nucleotide binding...
To identify mutants potentially defective in signaling intermediates specific to phytochrome A (phyA), we screened for extragenic mutations that suppress the morphological phenotype exhibited by a weak phyA mutant ( phyA-105 ) of Arabidopsis. A new recessive mutant, designated spa1 (for s...
Acidification of endomembrane compartments by the vacuolar-type H + -ATPase (V-ATPase) is central to many cellular processes in eukaryotes, including osmoregulation and protein sorting. The V-ATPase complex consists of a peripheral sector (V 1 ) and a membrane integral sector (V o ); however, it...
A method was designed for in vivo observation of sieve element/companion complexes by using confocal laser scanning microscopy. A leaf attached to an intact fava bean plant was mounted upside down on the stage of a confocal microscope. Two shallow paradermal cortical cuts were made in the major...
Plastids of nongreen tissues import carbon as a source of biosynthetic pathways and energy. Within plastids, carbon can be used in the biosynthesis of starch or as a substrate for the oxidative pentose phosphate pathway, for example. We have used maize endosperm to purify a plastidic glucose...
Dof is a novel family of plant proteins that share a unique and highly conserved DNA binding domain with one C 2 –C 2 zinc finger motif. Although multiple Dof proteins associated with diverse gene promoters have recently been identified in a variety of plants, their physiological functions and...
Because plants grow under many different types of soil and environmental conditions, we investigated the hypothesis that multiple pathways for K + uptake exist in plants. We have identified a new family of potassium transporters from Arabidopsis by searching for homologous sequences among the...
Plant roots contain both high- and low-affinity transport systems for uptake of K + from the soil. In this study, we characterize a K + transporter that functions in both high- and low-affinity uptake. Using yeast complementation analysis, we isolated a cDNA for a functional K + transporter from...
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