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The nuclei of two spherical bacteria have been examined in electron micrographs of thin sections of specimens prepared by the method of Ryter and Kellenberger (1958). The nuclei appear to consist of the same fine fibers in a matrix of low density which have already been seen in many other...
A simple procedure has been established for controlling and measuring the pH of media in which the bicarbonate-carbonic acid system is the predominant buffer. The HCO - 3 concentration was maintained at 22.5 mM and the H 2 CO 3 concentration was varied by equilibrating the media with 0.5 to 40...
The fine structure of the oxyntic cell from the gastric glands of the bullfrog was studied in lead hydroxide—stained sections of gastric mucosa fixed in buffered osmium tetroxide and embedded in n -butyl methacrylate. The oxyntic cell in non-acid-secreting stomachs (gastric juice pH, 7.4–7.8)...
Strain L cells accumulate lipid, concurrent with cessation of protein synthesis, in the stationary phase of growth from the extracellular medium and as a result of de novo synthesis. Cells which have been more severely damaged with an amino acid analogue also accumulate lipid from the...
A number of antigens in unfertilized eggs and embryos of the sea urchin Paracentrotus lividus were characterized with respect to both immunological and physicochemical properties. Experiments involved single diffusion in agar (Oudin technique) combined with mutual dilution, serial dilution, and...
In thin sections of Bacillus subtilis certain organelles are observed situated either in the nuclear area from where they can extend into the cytoplasm, or in contact with the cell wall. Inside the nuclear area, the organelle is sometimes composed of concentric layers each seen to consist of two...
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