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Allylsulfide dissolved in dimethyl sulfoxide (DMSO) has been used to distinguish between CH 4 oxidation and nitrification in organic soils and sediments. We studied if this method could be applied also to forest soils. However, in three upland forest soils 14 mM DMSO itself strongly (84–87%)...
Methods of determining soil microbial biomass need to be reliable and produce consistent results across soils with a wide range of properties. We investigated the effect of extractant molarity (distilled water and 0.001, 0.01, 0.1, and 0.5 M K 2 SO 4 ) on the flush of C (i.e. the difference...
Dilution cultures have advantages for studying small-celled oligotrophic bacteria because culture selection favors the most abundant, rather than the most nutrient-tolerant, organisms. In the present study, dilution cultures were employed to enrich small-celled (SC; <0.45 μm) and large-celled...
Cyanuric acid amendment was tested for potential enhancement of atrazine mineralization activity because this compound is the metabolite in the degradation pathway of s -triazines prior to ring-cleavage. The effect of cyanuric acid on: (i) subsequent atrazine mineralization kinetics and (ii) the...
We measured N 2 O production by two species of primary chemoautotrophic nitrifying bacteria, Nitrosomonas europaea and Nitrosolobus multiformis , in media with different dissolved O 2 concentrations. Both species produced N 2 O across a wide range of O 2 concentrations and after 48 h there was a...
The majority of bacterial cells in soil observed by fluorescence microscopy are less than 0.4 μm in diameter, yet these cells rarely are recovered on nutrient agar. Metabolically active bacteria that are not culturable on solid media might respond in BIOLOG ® microtitre plates, which contain 95...
A simplified method using centrifugation and microcentrifuge tubes instead of filtration to measure bacterial activity using thymidine or leucine incorporation, originally used in aquatic habitats, has been adapted for soil. A final step employing hot NaOH to solubilize macromolecules before...
Eubacterial community structures in the plant rhizosphere were examined with respect to plant species, soil type, and root zone location. Three plant species (chickpea, rape and Sudan grass) were grown in intact cores of three California soils (a sandy soil, a sandy loam, and a clay) and were...
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