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The effect of artificial Fusarium graminearum and F. culmorum infection at the level of the proteome on grains of naked barley (Hordeum vulgare subsp. nudum) was investigated in comparison to naturally infected samples. Fusarium infection in barley led to numerous host‐specific biochemical...
Topoisomerase (topo) II catalyzes topological changes in DNA. Although both human isozymes, topo IIα and β are phosphorylated, site‐specific phosphorylation of topo IIβ is poorly characterized. Using LC‐MS/MS analysis of topo IIβ, cleaved with trypsin, Arg C or cyanogen bromide (CNBr)...
Oxidative signalling by ROS has been demonstrated to play a role in seed dormancy alleviation, but the detailed molecular mechanisms underlying this process remain largely unknown. Here, we show dynamic differences in redox‐sensitive proteome upon wheat seed dormancy release. Using...
Current technologies for measuring protein expression across a tissue section are based on MS or in situ detection such as immunohistochemistry. However, due to the inherent molecular complexity of tissue samples and the large dynamic range of protein expression in cells, current approaches are...
Although microRNAs (miRNAs) have been reported to play an important role in carcinogenesis, their molecular mechanism remains largely unknown because of our limited understanding of miRNA target genes. miR‐373 was found to be capable of promoting breast cancer invasion and metastasis, but only...
To evaluate the implementation of various denaturants and their efficacy in bottom‐up membrane proteomic methods using LC‐MS analysis, microsomes isolated from tomato roots were treated with MS‐compatible surfactants (RapiGest SF Surfactant from Waters and PPS Silent Surfactant from Protein...
The aims of this study were to demonstrate the feasibility of centrally collecting and processing high‐quality cerebrospinal fluid (CSF) samples for proteomic studies within a multi‐center consortium and to identify putative biomarkers for medulloblastoma in CSF. We used 2‐DE to investigate...
A metal‐ion chelate immobilized enzyme reactor (IMER) supported on organic–inorganic hybrid silica monolith was developed for rapid digestion of proteins. The monolithic support was in situ prepared in a fused silica capillary via the polycondensation between tetraethoxysilane hydrolytic sol...
Puccinia triticina (Pt) is a representative of several cereal‐infecting rust fungal pathogens of major economic importance world wide. Upon entry through leaf stomata, these fungi establish intracellular haustoria, crucial feeding structures. We report the first proteome of infection structures...
We introduce a framework for predicting novel protein–protein interactions (PPIs), based on Fisher's method for combining probabilities of predictions that are based on different data sources, such as the biomedical literature, protein domain and mRNA expression information. Our method compares...
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