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Protein N‐terminal acetylation (Nα‐acetylation) is among the most common modifications in eukaryotes. We previously described a simple method to enrich Nα‐modified peptides using CNBr‐activated Sepharose resin. A limitation of this method is that an optimal ratio of sample to resin had...
The Cytochrome P450 (CYP) proteins are a family of membrane bound proteins that function as a major metabolizing enzyme in the human body. Quantification of CYP induction is critical in determining the disposition, safety and efficacy of drugs in humans. Described is a gel‐free,...
This review is a meta‐analysis of data describing proteins regulated by morphine influence studied worldwide across last years administration. Up to date (July 2010), 15 studies concerning this subject have been published. Animal models, examined brain structures, the route of morphine...
Large‐scale interaction studies contribute the largest fraction of protein interactions information in databases. However, co‐purification of non‐specific or indirect ligands, often results in data sets that are affected by a considerable number of false positives. For the fraction of...
Egg storage causes egg white to lose its viscous nature to form a thin liquid, commonly referred to as egg white thinning. To understand the mechanisms underlying egg white thinning, white‐shell eggs were used in the present study to determine the proteome‐level changes of egg white proteins...
Extremely acidophilic archaea from the genus Ferroplasma inhabit iron‐rich biomining environments and are important constituents of naturally occurring microbial consortia that catalyze the production of acid mine drainage. A combined bioinformatic, transcript profiling, and proteomic approach...
In this study, we applied a quantitative proteomic approach, based on SILAC, to investigate the interactions of coronaviruses with the secretory pathway of the host cell, with the aim to identify host factors involved in coronavirus replication. Comparison of the protein profiles of...
Serum amyloid P component (SAP) is a glycoprotein of interest due to its presence in amyloid plaque formations. As with most glycoproteins, SAP can possibly vary greatly in its isoforms, which can be an important factor toward understanding the role of SAP. Interestingly, previous...
The genus Pachycladon consists of ten species of alpine plants, nine of which are endemic to New Zealand. The species are closely related to the model plant Arabidopsis thaliana with respect to their sequence divergence and chromosome synteny, occupy distinct geographical habitats in terms of...
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