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The mature lipase of Burkholderia cepacia KWI-56 was synthesized in an enzymatically active form using an in vitro Escherichia coli S30 coupled transcription/translation system by expressing the mature lipase gene ( rlip ) in the presence of its specific activator. To investigate the substrate...
PACE4, furin and PC6 are Ca 2+ -dependent serine endoproteases that belong to the subtilisin-like proprotein convertase (SPC) family. Recent reports have supported the involvement of these enzymes in processing of growth/differentiation factors, viral replication, activation of bacterial toxins...
The Escherichia coli RrmJ gene product has recently been shown to be the 23S rRNA:U2552 specific 2′- O -ribose methyltransferase (MTase) (RrmJ). Its structure has been solved and refined to 1.5 Å resolution, demonstrating conservation of the three-dimensional fold and key catalytic side chains...
In this work, we propose a molecular model of the L-type calcium channel pore from the human cardiac α1 subunit. Four glutamic acid residues, the EEEE locus, located at highly conserved P loops (also called SS1–SS2 segments) of the α1 subunit, molecularly express the calcium channel...
The NADPH-dependent 2,5-diketo-D-gluconic acid (2,5-DKG) reductase enzyme is a required component in some novel biosynthetic vitamin C production processes. This enzyme catalyzes the conversion of 2,5-DKG to 2-keto-L-gulonic acid, which is an immediate precursor to L-ascorbic acid. Forty unique...
To understand the role of aromatic–aromatic interactions in imparting specificity to the folding process, the geometries of four aromatic residues with different sequence spacing, located in α-helices or five residues from helical ends, interacting with each other have been elucidated. The...
Current methods for identification of domains within protein sequences require either structural information or the identification of homologous domain sequences in different sequence contexts. Knowledge of structural domain boundaries is important for fold recognition experiments and structural...
Proper protein folding is key to producing recombinant proteins for structure determination. We have examined the effect of misfolded recombinant protein on gene expression in Escherichia coli . Comparison of expression patterns indicates a unique set of genes responding to translational...
We studied the effects of increase in the number of surface arginines on the enzyme activity and stability of Trichoderma reesei endo -1,4-β-xylanase II. The number of arginines was increased in two mutant series. The first set contained six arginines on different sides of the protein surface....
The score matrix from a structure comparison program (SAP) was used to search for repeated structures using a Fourier analysis. When tested with artificial data, a simple Fourier transform of the smoothed matrix provided a clear signal of the repeat periodicity that could be used to extract the...
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